Construct: ORF TRCN0000476780
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018520.1_s317c1
- Derived from:
- ccsbBroadEn_07907
- DNA Barcode:
- GTGCCTCATCAACCGGTTTTGGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LPAR3 (23566)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476780
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23566 | LPAR3 | lysophosphatidic acid recep... | NM_012152.3 | 99.8% | 99.7% | 139_140delTCinsNN |
| 2 | human | 23566 | LPAR3 | lysophosphatidic acid recep... | XM_024446128.1 | 99.8% | 99.7% | 139_140delTCinsNN |
| 3 | human | 23566 | LPAR3 | lysophosphatidic acid recep... | XM_024446127.1 | 96.7% | 96.7% | 1_33del;172_173delTCinsNN |
| 4 | mouse | 65086 | Lpar3 | lysophosphatidic acid recep... | NM_022983.4 | 84.5% | 90.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1128
- ORF length:
- 1059
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaatgagtgt cactatgaca agcacatgga ctttttttat aataggagca 121 acactgatac tgtcgatgac tggacaggaa caaagcttgt gattgttttg tgtgttggga 181 cgtttttctg cctgtttatt ttttttnnta attctctggt catcgcggca gtgatcaaaa 241 acagaaaatt tcatttcccc ttctactacc tgttggctaa tttagctgct gccgatttct 301 tcgctggaat tgcctatgta ttcctgatgt ttaacacagg cccagtttca aaaactttga 361 ctgtcaaccg ctggtttctc cgtcaggggc ttctggacag tagcttgact gcttccctca 421 ccaacttgct ggttatcgcc gtggagaggc acatgtcaat catgaggatg cgggtccata 481 gcaacctgac caaaaagagg gtgacactgc tcattttgct tgtctgggcc atcgccattt 541 ttatgggggc ggtccccaca ctgggctgga attgcctctg caacatctct gcctgctctt 601 ccctggcccc catttacagc aggagttacc ttgttttctg gacagtgtcc aacctcatgg 661 ccttcctcat catggttgtg gtgtacctgc ggatctacgt gtacgtcaag aggaaaacca 721 acgtcttGTC TCCGCATACA AGTGGGTCCA TCAGCCGCCG GAGGACACCC ATGAAGCTAA 781 TGAAGACGGT GATGACTGTC TTAGGGGCGT TTGTGGTATG CTGGACCCCG GGCCTGGTGG 841 TTCTGCTCCT CGACGGCCTG AACTGCAGGC AGTGTGGCGT GCAGCATGTG AAAAGGTGGT 901 TCCTGCTGCT GGCGCTGCTC AACTCCGTCG TGAACCCCAT CATCTACTCC TACAAGGACG 961 AGGACATGTA TGGCACCATG AAGAAGATGA TCTGCTGCTT CTCTCAGGAG AACCCAGAGA 1021 GGCGTCCCTC TCGCATCCCC TCCACAGTCC TCAGCAGGAG TGACACAGGC AGCCAGTACA 1081 TAGAGGATAG TATTAGCCAA GGTGCAGTCT GCAATAAAAG CACTTCCTTG ACAACTTTCT 1141 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1201 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1261 GTGGAAAGGA CGAGTGCCTC ATCAACCGGT TTTGGGTACG CGTTAAGTCg acaatcaacc 1321 tctggattac aaaatttgtg aaagatt