Transcript: Human XM_024446128.1

PREDICTED: Homo sapiens lysophosphatidic acid receptor 3 (LPAR3), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
LPAR3 (23566)
Length:
3404
CDS:
98..1159

Additional Resources:

NCBI RefSeq record:
XM_024446128.1
NBCI Gene record:
LPAR3 (23566)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024446128.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000356956 CTACTACCTGTTGGCTAATTT pLKO_005 292 CDS 100% 15.000 21.000 N LPAR3 n/a
2 TRCN0000011393 GCTGCTCAACTCCGTCGTGAA pLKO.1 943 CDS 100% 1.350 1.890 N LPAR3 n/a
3 TRCN0000221143 GCCGATTTCTTCGCTGGAATT pLKO.1 320 CDS 100% 0.000 0.000 N LPAR3 n/a
4 TRCN0000356890 CAGTACATAGAGGATAGTATT pLKO_005 1103 CDS 100% 13.200 9.240 N LPAR3 n/a
5 TRCN0000356888 CCATTAATCACTGCTAGATTT pLKO_005 1264 3UTR 100% 13.200 9.240 N LPAR3 n/a
6 TRCN0000221145 CCTCACCAACTTGCTGGTTAT pLKO.1 445 CDS 100% 10.800 7.560 N LPAR3 n/a
7 TRCN0000221144 GCCTATGTATTCCTGATGTTT pLKO.1 341 CDS 100% 5.625 3.938 N LPAR3 n/a
8 TRCN0000221146 GAAGCTAATGAAGACGGTGAT pLKO.1 802 CDS 100% 4.050 2.835 N LPAR3 n/a
9 TRCN0000357019 GATTACCTGTCTCTAACAAAG pLKO_005 1217 3UTR 100% 10.800 6.480 N LPAR3 n/a
10 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 2293 3UTR 100% 13.200 6.600 Y LIAS n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024446128.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489572 TATCCTGTTATTGTAACTTCGCAC pLX_317 38% 100% 100% V5 (not translated due to prior stop codon) n/a
2 TRCN0000489136 TACCTGTCTAAGAGGGGCAGCTTA pLX_317 30.5% 99.9% 99.7% V5 1059_1060insG n/a
3 ccsbBroadEn_07907 pDONR223 99.8% 99.8% 99.7% None 139_140delTCinsNN n/a
4 ccsbBroad304_07907 pLX_304 0% 99.8% 99.7% V5 139_140delTCinsNN n/a
5 TRCN0000476780 GTGCCTCATCAACCGGTTTTGGGT pLX_317 37.9% 99.8% 99.7% V5 139_140delTCinsNN n/a
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