Construct: ORF TRCN0000476803
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017668.1_s317c1
- Derived from:
- ccsbBroadEn_05875
- DNA Barcode:
- GCCACACGACGTAACACCACCTGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AVPR2 (554)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476803
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 554 | AVPR2 | arginine vasopressin recept... | NM_000054.6 | 99.9% | 100% | 927A>G |
| 2 | human | 554 | AVPR2 | arginine vasopressin recept... | NM_001146151.3 | 82.6% | 82.7% | (many diffs) |
| 3 | human | 554 | AVPR2 | arginine vasopressin recept... | NR_027419.2 | 51.4% | (many diffs) | |
| 4 | mouse | 12000 | Avpr2 | arginine vasopressin recept... | NM_019404.2 | 84% | 87.6% | (many diffs) |
| 5 | mouse | 12000 | Avpr2 | arginine vasopressin recept... | XM_006527756.3 | 84% | 87.6% | (many diffs) |
| 6 | mouse | 12000 | Avpr2 | arginine vasopressin recept... | XM_017318365.1 | 83.9% | 87.3% | (many diffs) |
| 7 | mouse | 12000 | Avpr2 | arginine vasopressin recept... | NM_001276299.1 | 35.7% | 37.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1182
- ORF length:
- 1113
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctcatggcg tccaccactt ccgctgtgcc tgggcatccc tctctgccca 121 gcctgcccag caacagcagc caggagaggc cactggacac ccgggacccg ctgctagccc 181 gggcggagct ggcgctgctc tccatagtct ttgtggctgt ggccctgagc aatggcctgg 241 tgctggcggc cctagctcgg cggggccggc ggggccactg ggcacccata cacgtcttca 301 ttggccactt gtgcctggcc gacctggccg tggctctgtt ccaagtgctg ccccagctgg 361 cctggaaggc caccgaccgc ttccgtgggc cagatgccct gtgtcgggcc gtgaagtatc 421 tgcagatggt gggcatgtat gcctcctcct acatgatcct ggccatgacg ctggaccgcc 481 accgtgccat ctgccgtccc atgctggcgt accgccatgg aagtggggct cactggaacc 541 ggccggtgct agtggcttgg gccttctcgc tccttctcag cctgccccag ctcttcatct 601 tcgcccagcg caacgtggaa ggtggcagcg gggtcactga ctgctgggcc tgctttgcgg 661 agccctgggg ccgtcgcacc tatgtcacct ggattgccct gatggtgttc gtggcaccta 721 ccctgggtat cgccgcctgc caggtgctca tcttccggga gattcatgcc agtctggtgc 781 cagggccatc agagaggcct ggggggcgcc gcaggggacg ccggacaggc agccccggtg 841 agggagccca cgtgtcagca gctgtggcca agactgtgag gatgacgcta gtgattgtgg 901 tcgtctatgt gctgtgctgg gcacccttct tcctggtgca gctgtgggcc gcgtgggacc 961 cggaggcacc tctggaaggg gcgccctttg tgctgctcat gttgctggcc agcctcaaca 1021 gctgcaccaa cccctggatc tatgcatctt tcagcagcag cgtgtcctca gagctgcgaa 1081 gcttgctcTG CTGTGCCCGG GGACGCACCC CACCCAGCCT GGGTCCCCAA GATGAGTCCT 1141 GCACCACCGC CAGCTCCTCC CTGGCCAAGG ACACTTCATC GTTGCCAACT TTCTTGTACA 1201 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1261 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1321 AGGACGAGCC ACACGACGTA ACACCACCTG GACGCGTTAA GTCgacaatc aacctctgga 1381 ttacaaaatt tgtgaaagat t