Construct: ORF TRCN0000476805
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012773.1_s317c1
- Derived from:
- ccsbBroadEn_01157
- DNA Barcode:
- TCTCCTGTATCATCGGCTATGTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCYT1A (5130)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476805
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5130 | PCYT1A | phosphate cytidylyltransfer... | NM_001312673.2 | 100% | 100% | |
| 2 | human | 5130 | PCYT1A | phosphate cytidylyltransfer... | NM_005017.4 | 100% | 100% | |
| 3 | mouse | 13026 | Pcyt1a | phosphate cytidylyltransfer... | NM_001163159.1 | 89.7% | 96.1% | (many diffs) |
| 4 | mouse | 13026 | Pcyt1a | phosphate cytidylyltransfer... | NM_001163160.1 | 89.7% | 96.1% | (many diffs) |
| 5 | mouse | 13026 | Pcyt1a | phosphate cytidylyltransfer... | NM_009981.4 | 89.7% | 96.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1167
- ORF length:
- 1101
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tgcacagtgt tcagccaagg tcaatgcaag gaagaggaga aaagaggcgc 121 ccggacccaa cggggcaaca gaagaagatg gggttccttc caaagtgcag cgctgtgcag 181 tgggcttacg gcaaccagct cctttttctg atgaaattga agttgacttt agtaagccct 241 atgtcagggt aactatggaa gaagccagca gaggaactcc ttgtgagcga cctgtgagag 301 tttatgccga tggaatattt gacttatttc actctggtca cgcccgagct ctgatgcaag 361 cgaagaacct tttccctaat acgtacctca ttgtgggagt ttgcagtgat gagctcacac 421 acaacttcaa aggcttcacg gtgatgaacg agaatgagcg ctatgacgca gtccagcact 481 gccgctacgt ggatgaggtg gtgaggaatg cgccctggac gctgacaccc gagttcctgg 541 ccgaacaccg gattgatttt gtagcccatg atgatattcc ttattcatct gctggcagtg 601 atgatgttta taagcacatc aaggaggcag gcatgtttgc tccaacacag aggacagaag 661 gtatctccac atcagacatc atcacccgaa ttgtgcggga ttatgatgtg tatgcgaggc 721 ggaacctgca gaggggctac acagcaaagg agctcaatgt cagctttatc aacgagaaga 781 aataccactt gcaggagagg gttgacaaag taaagaagaa agtgaaagat gtggaggaaa 841 agtcaaaaga atttgttcag aaggtggagg aaaaaagcat tgaccTCATT CAGAAGTGGG 901 AGGAGAAGTC CCGAGAATTC ATTGGAAGTT TTCTGGAAAT GTTTGGTCCG GAAGGAGCAC 961 TGAAACATAT GCTGAAAGAG GGGAAGGGCC GGATGCTGCA GGCCATCAGC CCGAAGCAGA 1021 GCCCCAGCAG CAGCCCTACT CGCGAGCGCT CCCCCTCCCC CTCTTTCCGA TGGCCCTTCT 1081 CCGGCAAGAC TTCCCCACCT TGCTCCCCAG CAAATCTCTC CAGGCACAAG GCTGCAGCCT 1141 ATGATATCAG TGAGGATGAA GAAGACTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 1201 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1261 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATCTCCTGT 1321 ATCATCGGCT ATGTATACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1381 aagatt