Construct: ORF TRCN0000476829
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014280.1_s317c1
- Derived from:
- ccsbBroadEn_08384
- DNA Barcode:
- GTGTCGCTTGACTGTGAATTTATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRMT13 (54482)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476829
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54482 | TRMT13 | tRNA methyltransferase 13 h... | NM_019083.2 | 99.8% | 99.7% | 142_143delGCinsAT |
| 2 | human | 54482 | TRMT13 | tRNA methyltransferase 13 h... | XM_005270945.3 | 96.9% | 96.8% | 142_143delGCinsAT;392_393ins42 |
| 3 | human | 54482 | TRMT13 | tRNA methyltransferase 13 h... | XM_005270946.2 | 70.6% | 70.6% | 0_1ins423 |
| 4 | human | 54482 | TRMT13 | tRNA methyltransferase 13 h... | XR_002956838.1 | 67% | (many diffs) | |
| 5 | human | 54482 | TRMT13 | tRNA methyltransferase 13 h... | XM_017001504.1 | 63.3% | 57.7% | (many diffs) |
| 6 | human | 54482 | TRMT13 | tRNA methyltransferase 13 h... | XM_017001505.1 | 60.4% | 54.8% | (many diffs) |
| 7 | human | 54482 | TRMT13 | tRNA methyltransferase 13 h... | XR_246275.3 | 56.3% | (many diffs) | |
| 8 | human | 54482 | TRMT13 | tRNA methyltransferase 13 h... | XR_001737238.1 | 42.2% | (many diffs) | |
| 9 | human | 54482 | TRMT13 | tRNA methyltransferase 13 h... | XR_246273.4 | 39.7% | (many diffs) | |
| 10 | human | 54482 | TRMT13 | tRNA methyltransferase 13 h... | NR_135078.1 | 38% | (many diffs) | |
| 11 | human | 54482 | TRMT13 | tRNA methyltransferase 13 h... | XR_001737240.1 | 35.9% | (many diffs) | |
| 12 | human | 54482 | TRMT13 | tRNA methyltransferase 13 h... | XR_001737236.1 | 32% | (many diffs) | |
| 13 | human | 54482 | TRMT13 | tRNA methyltransferase 13 h... | XR_001737237.1 | 30.9% | (many diffs) | |
| 14 | human | 54482 | TRMT13 | tRNA methyltransferase 13 h... | XR_001737239.1 | 29.4% | (many diffs) | |
| 15 | mouse | 229780 | Trmt13 | tRNA methyltransferase 13 | NM_030016.2 | 85.7% | 84.8% | (many diffs) |
| 16 | mouse | 229780 | Trmt13 | tRNA methyltransferase 13 | XM_011240110.2 | 74.8% | 74.2% | (many diffs) |
| 17 | mouse | 229780 | Trmt13 | tRNA methyltransferase 13 | XM_011240111.1 | 71.1% | 70.3% | (many diffs) |
| 18 | mouse | 229780 | Trmt13 | tRNA methyltransferase 13 | XM_006501424.1 | 60.9% | 60.1% | (many diffs) |
| 19 | mouse | 229780 | Trmt13 | tRNA methyltransferase 13 | XM_011240112.2 | 60.9% | 60.1% | (many diffs) |
| 20 | mouse | 229780 | Trmt13 | tRNA methyltransferase 13 | XR_375521.2 | 56.7% | (many diffs) | |
| 21 | mouse | 229780 | Trmt13 | tRNA methyltransferase 13 | XM_017319564.1 | 49.9% | 50% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1512
- ORF length:
- 1443
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgacctcc gcgacgtcgc cgcacgcgcc tggttttcca gctgagggta 121 gatgcggtta ctatgtggaa aagaagaaac ggttctgcag gatggtggtg gccgcaggga 181 aaagattttg tggtgaacac gctggagcca tggaggaaga agatgctcgg aaaagaatcc 241 tgtgtccttt agatccaaaa cacacagtat atgaagatca actagcaaag catttgaaaa 301 aatgtaactc aagagagaaa ccaaaacctg atttctatat tcaagatatt aatgcaggct 361 taagagatga aacagaaata cctgaacaat tagttccaat ttcttctcta tctgaagagc 421 agttggaaaa gttaattaag aaattgagaa aagcaagtga aggcttgaat tctacactta 481 aagatcatat tatgtcccat ccagcattac acgatgcact taatgaccct aaaaatggcg 541 attctgcaac caagcacctg aaacagcagg cttctatttt aggtaacatt gaaaatttaa 601 agttacttgg tccaagaaga tgctttgttg agtttggagc gggaaaggga aaattatctc 661 attgggttga tattgcctta aaagatgctg aaaaagttca cttcatccta gtggaaaagg 721 tgaccacaag attcaaggtg gatggaaaac acagaaagaa aaattcagtg tttgaaagac 781 ttcaaattga tattcaacac ttgtgtttga acaagattcc tgtgctaaga gaagaaaaac 841 tacctgtggt aggaattgga aagcatctgt gtggtatggc aacagatctt gcattacgat 901 gtttggttga aacctatgct gccagttttg aggaaaggaa tgaagaacct ttagccaaac 961 gcataaagaa tgataaaaca gaaaaagaaa tttacacttt ggccaaggaa ggaaatgaaa 1021 aaaatgtccc agagaagtgg aaccctgtgg ctggcattgt tattgcactc tgttgtcacc 1081 acaggtgtga ttggagacat tatgtgggca aagaatattt cagggctcta ggccttggag 1141 cagtggaatt ccattatttc cagcgaaTGA GTAGTTGGGC AACTTGTGGG ATGCGGAAAA 1201 CATCTTTGGA AACCTCAAAT AGTACCACAA AGAGGCAAGA TAATCAGAAT GATGATAGTG 1261 AAGAGCATGA TGATGGAGGA TACAGAATCA CAGATGATGG CGCTGATTGT TTGCCTGGGC 1321 TTCTTAGTGT TGAAGAAAAG AAGAAAATAG GGCATCTTTG TAAATTGCTG ATTGACCAAG 1381 GTCGAATCCA GTATTTGCAG CAGAAGGGAT TCAGTCCTGC TTTGCAGTAC TATACAGACC 1441 CTCTGGTGTC TTTGGAAAAT GTTTTGTTAA CTGCTTTACC AAATCATTCT TCATCACCAG 1501 AAACAACTGC TTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1561 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1621 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGTG TCGCTTGACT GTGAATTTAT 1681 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t