Construct: ORF TRCN0000476840
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008371.1_s317c1
- Derived from:
- ccsbBroadEn_04560
- DNA Barcode:
- GACACTCATTATGTGTGCCGTCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LIN52 (91750)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476840
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 91750 | LIN52 | lin-52 DREAM MuvB core comp... | NM_001024674.3 | 96.5% | 96.5% | 0_1insATGGGTTGGAAG |
| 2 | human | 91750 | LIN52 | lin-52 DREAM MuvB core comp... | NM_001372006.1 | 94.8% | 94.8% | 0_1insATGGGTTGGAAG;18_19insGGGACA |
| 3 | human | 91750 | LIN52 | lin-52 DREAM MuvB core comp... | XM_011537322.2 | 87.8% | 86.4% | (many diffs) |
| 4 | human | 91750 | LIN52 | lin-52 DREAM MuvB core comp... | XM_017021764.1 | 84.4% | 84.4% | 294_295ins54 |
| 5 | human | 91750 | LIN52 | lin-52 DREAM MuvB core comp... | XM_011537321.3 | 68.6% | 67.5% | (many diffs) |
| 6 | human | 91750 | LIN52 | lin-52 DREAM MuvB core comp... | NM_001372005.1 | 68.6% | 65.5% | (many diffs) |
| 7 | mouse | 217708 | Lin52 | lin-52 homolog (C. elegans) | NM_173756.4 | 91.3% | 100% | (many diffs) |
| 8 | mouse | 629959 | Gm7020 | predicted gene 7020 | XM_006534617.3 | 91% | 99.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 417
- ORF length:
- 348
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttGGCACCAT GGGTTGGAAG ATGGCGTCTC CCACAGACGG GACAGATCTG GAAGCATCTT 121 TGCTAAGTTT TGAAAAACTT GACCGTGCCT CACCAGATCT TTGGCCAGAA CAATTACCAG 181 GTGTTGCTGA ATTTGCAGCT TCCTTCAAAA GTCCTATTAC TAGTTCTCCA CCCAAATGGA 241 TGGCTGAGAT AGAACGTGAT GACATCGACA TGTTGAAAGA ACTGGGGAGT CTCACCACGG 301 CTAATTTGAT GGAGAAGGTT CGAGGCCTAC AGAACCTAGC CTATCAGCTG GGGCTGGATG 361 AGTCCAGAGA GATGACACGG GGGAAATTCC TCAATATTCT AGAGAAGCCC AAGAAGTTGC 421 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 481 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTGAAAGT ATTTCGATTT CTTGGCTTTA 541 TATATCTTGT GGAAAGGACG AGACACTCAT TATGTGTGCC GTCCAACGCG TTAAGTCgac 601 aatcaacctc tggattacaa aatttgtgaa agatt