Transcript: Human XM_011537321.3

PREDICTED: Homo sapiens lin-52 DREAM MuvB core complex component (LIN52), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
LIN52 (91750)
Length:
1151
CDS:
1..435

Additional Resources:

NCBI RefSeq record:
XM_011537321.3
NBCI Gene record:
LIN52 (91750)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011537321.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000115878 CACGGCTAATTTGATGGAGAA pLKO.1 324 CDS 100% 4.050 5.670 N LIN52 n/a
2 TRCN0000115877 CCAGAACAATTACCAGGTGTT pLKO.1 193 CDS 100% 4.050 3.240 N LIN52 n/a
3 TRCN0000115881 GAGTCTCACCACGGCTAATTT pLKO.1 315 CDS 100% 15.000 10.500 N LIN52 n/a
4 TRCN0000286168 GAGTCTCACCACGGCTAATTT pLKO_005 315 CDS 100% 15.000 10.500 N LIN52 n/a
5 TRCN0000293560 ACCGTGCCTCACCAGATCTTT pLKO_005 170 CDS 100% 5.625 3.938 N LIN52 n/a
6 TRCN0000115879 TGGAAGCATCTTTGCTAAGTT pLKO.1 137 CDS 100% 5.625 3.938 N LIN52 n/a
7 TRCN0000286170 TGGAAGCATCTTTGCTAAGTT pLKO_005 137 CDS 100% 5.625 3.938 N LIN52 n/a
8 TRCN0000115880 GTGTTGCTGAATTTGCAGCTT pLKO.1 209 CDS 100% 2.640 1.848 N LIN52 n/a
9 TRCN0000286169 GTGTTGCTGAATTTGCAGCTT pLKO_005 209 CDS 100% 2.640 1.848 N LIN52 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011537321.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04560 pDONR223 100% 68.6% 67.5% None (many diffs) n/a
2 ccsbBroad304_04560 pLX_304 0% 68.6% 67.5% V5 (many diffs) n/a
3 TRCN0000476840 GACACTCATTATGTGTGCCGTCCA pLX_317 98.3% 68.6% 67.5% V5 (many diffs) n/a
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