Construct: ORF TRCN0000476849
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001000.1_s317c1
- Derived from:
- ccsbBroadEn_05900
- DNA Barcode:
- GGCCCGTGTTTACACACAAGAATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BMX (660)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476849
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 660 | BMX | BMX non-receptor tyrosine k... | NM_001721.7 | 99.9% | 100% | 9A>G |
| 2 | human | 660 | BMX | BMX non-receptor tyrosine k... | NM_203281.3 | 99.9% | 100% | 9A>G |
| 3 | human | 660 | BMX | BMX non-receptor tyrosine k... | NM_001320866.2 | 99.8% | 99.8% | 9A>G;750_751insAGT |
| 4 | human | 660 | BMX | BMX non-receptor tyrosine k... | XM_017029752.2 | 70.6% | 68.2% | 9A>G;1394_1395ins217;1431_1432ins377 |
| 5 | mouse | 12169 | Bmx | BMX non-receptor tyrosine k... | NM_009759.4 | 85.3% | 87% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2091
- ORF length:
- 2025
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tacgaaatct attctagaag aacttcttct caaaagatca cagcaaaaga 121 agaaaatgtc accaaataat tacaaagaac ggctttttgt tttgaccaaa acaaaccttt 181 cctactatga atatgacaaa atgaaaaggg gcagcagaaa aggatccatt gaaattaaga 241 aaatcagatg tgtggagaaa gtaaatctcg aggagcagac gcctgtagag agacagtacc 301 catttcagat tgtctataaa gatgggcttc tctatgtcta tgcatcaaat gaagagagcc 361 gaagtcagtg gttgaaagca ttacaaaaag agataagggg taacccccac ctgctggtca 421 agtaccatag tgggttcttc gtggacggga agttcctgtg ttgccagcag agctgtaaag 481 cagccccagg atgtaccctc tgggaagcat atgctaatct gcatactgca gtcaatgaag 541 agaaacacag agttcccacc ttcccagaca gagtgctgaa gatacctcgg gcagttcctg 601 ttctcaaaat ggatgcacca tcttcaagta ccactctagc ccaatatgac aacgaatcaa 661 agaaaaacta tggctcccag ccaccatctt caagtaccag tctagcgcaa tatgacagca 721 actcaaagaa aatctatggc tcccagccaa acttcaacat gcagtatatt ccaagggaag 781 acttccctga ctggtggcaa gtaagaaaac tgaaaagtag cagcagcagt gaagatgttg 841 caagcagtaa ccaaaaagaa agaaatgtga atcacaccac ctcaaagatt tcatgggaat 901 tccctgagtc aagttcatct gaagaagagg aaaacctgga tgattatgac tggtttgctg 961 gtaacatctc cagatcacaa tctgaacagt tactcagaca aaagggaaaa gaaggagcat 1021 ttatggttag aaattcgagc caagtgggaa tgtacacagt gtccttattt agtaaggctg 1081 tgaatgataa aaaaggaact gtcaaacatt accacgtgca tacaaatgct gagaacaaat 1141 tatacctggc agaaaactac tgttttgatt ccattccaaa gcttattcat tatcatcaac 1201 acaattcagc aggcatgatc acacggctcc gccaccctgt gtcaacaaag gccaacaagg 1261 tccccgactc tgtgtccctg ggaaatggaa tctgggaact gaaaagagaa gagattacct 1321 tgttgaagga gctgggaagt ggccagtttg gagtggtcca gctgggcaag tggaaggggc 1381 agtatgatgt tgctgttaag atgatcaagg agggctccat gtcagaagat gaattctttc 1441 aggaggccca gactatgatg aaactcagcc atcccaagct ggttaaattc tatggagtgt 1501 gttcaaagga ataccccata tacatagtga ctgaatatat aagcaatggc tgcttgctga 1561 attacctgag gagtcacgga aaaggacttg aaccttccca gctcttagaa atgtgctacg 1621 atgtctgtga aggcatggcc ttcttggaga gtcaccaatt catacaccgg gacttggctg 1681 ctcgtaactg cttggtggac agagatctct gtgtgaaagt aTCTGACTTT GGAATGACAA 1741 GGTATGTTCT TGATGACCAG TATGTCAGTT CAGTCGGAAC AAAGTTTCCA GTCAAGTGGT 1801 CAGCTCCAGA GGTGTTTCAT TACTTCAAAT ACAGCAGCAA GTCAGACGTA TGGGCATTTG 1861 GGATCCTGAT GTGGGAGGTG TTCAGCCTGG GGAAGCAGCC CTATGACTTG TATGACAACT 1921 CCCAGGTGGT TCTGAAGGTC TCCCAGGGCC ACAGGCTTTA CCGGCCCCAC CTGGCATCGG 1981 ACACCATCTA CCAGATCATG TACAGCTGCT GGCACGAGCT TCCAGAAAAG CGTCCCACAT 2041 TTCAGCAACT CCTGTCTTCC ATTGAACCAC TTCGGGAAAA AGACAAGCAT TGCCCAACTT 2101 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2161 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2221 CTTGTGGAAA GGACGAGGCC CGTGTTTACA CACAAGAATT ACGCGTTAAG TCgacaatca 2281 acctctggat tacaaaattt gtgaaagatt