Construct: ORF TRCN0000476865
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007738.1_s317c1
- Derived from:
- ccsbBroadEn_14047
- DNA Barcode:
- GGCAGTACACACTTCACTTACACA
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TACC3 (10460)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476865
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10460 | TACC3 | transforming acidic coiled-... | NM_006342.3 | 99.9% | 99.7% | 1689C>T;2508delG |
| 2 | human | 10460 | TACC3 | transforming acidic coiled-... | XM_017007653.1 | 99.9% | 99.7% | 1689C>T;2508delG |
| 3 | human | 10460 | TACC3 | transforming acidic coiled-... | XM_005247929.3 | 99.4% | 99.2% | 1689C>T;1941_1952del;2520delG |
| 4 | human | 10460 | TACC3 | transforming acidic coiled-... | XM_011513386.3 | 84.1% | 81.6% | (many diffs) |
| 5 | human | 10460 | TACC3 | transforming acidic coiled-... | XM_005247930.3 | 56.6% | 56.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2583
- ORF length:
- 2514
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagtctgcag gtcttaaacg acaaaaatgt cagcaatgaa aaaaatacag 121 aaaattgcga cttcctgttt tcgccaccag aagttaccgg aagatcgtct gttcttcgtg 181 tgtcacagaa agaaaatgtg ccacccaaga acctggccaa agctatgaag gtgacttttc 241 agacacctct gcgggatcca cagacgcaca ggattctaag tcctagcatg gccagcaaac 301 ttgaggctcc tttcactcag gatgacaccc ttggactgga aaactcacac ccggtctgga 361 cacagaaaga gaaccaacag ctcatcaagg aagtggatgc caaaactact catggaattc 421 tacagaaacc agtggaggct gacaccgacc tcctggggga tgcaagccca gcctttggga 481 gtggcagctc cagcgagtct ggcccaggtg ccctggctga cctggactgc tcaagctctt 541 cccagagccc aggaagttct gagaaccaaa tggtgtctcc aggaaaagtg tctggcagcc 601 ctgagcaagc cgtggaggaa aaccttagtt cctattcctt agacagaaga gtgacacccg 661 cctctgagac cctagaagac ccttgcagga cagagtccca gcacaaagcg gagactccgc 721 acggagccga ggaagaatgc aaagcggaga ctccgcacgg agccgaggag gaatgccggc 781 acggtggggt ctgtgctccc gcagcagtgg ccacttcgcc tcctggtgca atccctaagg 841 aagcctgcgg aggagcaccc ctgcagggtc tgcctggcga agccctgggc tgccctgcgg 901 gtgtgggcac ccccgtgcca gcagatggca ctcagaccct tacctgtgca cacacctctg 961 ctcctgagag cacagcccca accaaccacc tggtggctgg cagggccatg accctgagtc 1021 ctcaggaaga agtggctgca ggccaaatgg ccagctcctc gaggagcgga cctgtaaaac 1081 tagaatttga tgtatctgat ggcgccacca gcaaaagggc acccccacca aggagactgg 1141 gagagaggtc cggcctcaag cctcccttga ggaaagcagc agtgaggcag caaaaggccc 1201 cgcaggaggt ggaggaggac gacggtagga gcggagcagg agaggacccc cccatgccag 1261 cttctcgggg ctcttaccac ctcgactggg acaaaatgga tgacccaaac ttcatcccgt 1321 tcggaggtga caccaagtct ggttgcagtg aggcccagcc cccagaaagc cctgagacca 1381 ggctgggcca gccagcggct gaacagttgc atgctgggcc tgccacggag gagccaggtc 1441 cctgtctgag ccagcagctg cattcagcct cagcggagga cacgcctgtg gtgcagttgg 1501 cagccgagac cccaacagca gagagcaagg agagagcctt gaactctgcc agcacctcgc 1561 ttcccacaag ctgtccaggc agtgagccag tgcccaccca tcagcagggg cagcctgcct 1621 tggagctgaa agaggagagc ttcagagacc ccgctgaggt tctaggcacg ggcgcggagg 1681 tggattacct ggagcagttt ggaacttcct cgtttaagga gtcggccttg aggaagcagt 1741 ccttatacct caagtttgac cccctcctga gggacagtcc tggtagacca gtgcccgtgg 1801 ccaccgagac cagcagcatg cacggtgcaa atgagactcc ctcaggacgt ccgcgggaag 1861 ccaagcttgt ggagttcgat ttcttgggag cactggacat tcctgtgcca ggcccacccc 1921 caggtgttcc cgcgcctggg ggcccacccc tgtccaccgg acctatagtg gacctgctcc 1981 agtacagcca gaaggacctg gatgcagtgg taaaggcgac acaggaggag aaccgggagc 2041 tgaggagcag gtgtgaggag ctccacggga agaacctgga actggggaag atcatggaca 2101 ggttcgaaga ggttgtgtac caggccatgg aggaagttca gaagcagaag gaactttcca 2161 aagctgaaat ccagaaagtt ctaaaagaaa aagaccaact taccacagat ctgaactcca 2221 tggagaagtc cttcTCCGAC CTCTTCAAGC GTTTTGAGAA ACAGAAAGAG GTGATCGAGG 2281 GCTACCGCAA GAACGAAGAG TCACTGAAGA AGTGCGTGGA GGATTACCTG GCAAGGATCA 2341 CCCAGGAGGG CCAGAGGTAC CAAGCCCTGA AGGCCCACGC GGAGGAGAAG CTGCAGCTGG 2401 CAAACGAGGA GATCGCCCAG GTCCGGAGCA AGGCCCAGGC GGAAGCGTTG GCCCTCCAGG 2461 CCAGCCTGAG GAAGGAGCAG ATGCGCATCC AGTCGCTGGA GAAGACAGTG GAGCAGAAGA 2521 CTAAAGAGAA CGAGGAGCTG ACCAGGATCT GCGACGACCT CATCTCCAAG ATGGAAAGAT 2581 CTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT 2641 CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG 2701 GCTTTATATA TCTTGTGGAA AGGACGAGGC AGTACACACT TCACTTACAC AACGCGTTAA 2761 GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t