Construct: ORF TRCN0000476866
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007184.1_s317c1
- Derived from:
- ccsbBroadEn_09261
- DNA Barcode:
- AGGACGTCATAGCTCCTTAAATTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NKD1 (85407)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476866
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 85407 | NKD1 | NKD inhibitor of WNT signal... | NM_033119.5 | 99.8% | 99.5% | 217G>T;1130C>T |
| 2 | human | 85407 | NKD1 | NKD inhibitor of WNT signal... | XM_011523399.2 | 83.6% | 82.3% | (many diffs) |
| 3 | human | 85407 | NKD1 | NKD inhibitor of WNT signal... | XM_011523400.2 | 83.6% | 82.3% | (many diffs) |
| 4 | human | 85407 | NKD1 | NKD inhibitor of WNT signal... | XM_017023804.1 | 83.6% | 82.3% | (many diffs) |
| 5 | mouse | 93960 | Nkd1 | naked cuticle 1 | NM_027280.3 | 85.7% | 87% | (many diffs) |
| 6 | mouse | 93960 | Nkd1 | naked cuticle 1 | NM_001163660.1 | 67.3% | 67.7% | (many diffs) |
| 7 | mouse | 93960 | Nkd1 | naked cuticle 1 | XM_030243873.1 | 67.3% | 67.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1479
- ORF length:
- 1410
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggggaaactt cactccaagc cggccgccgt gtgcaagcgc agggagagcc 121 cggaaggtga cagcttcgcc gtgagcgctg cctgggctcg gaagggcatc gaggagtgga 181 tcgggagaca gcgctgcccg ggcggtgtct cgggaccccg acagctgcgg ttggcgggca 241 ccataggccg aagcacccgg gagctcgtgg gcgacgtgtt gagatacacg ctcagcgagg 301 aagaggagga cgactttcgg ctggaagtgg ccctgcctcc tgagaagact gacgggctgg 361 gcagcggaga tgagaagaag atggagagag tgagcgaacc ctgcccaggc tccaagaagc 421 agctgaagtt tgaagagctc cagtgcgacg tgtccatgga ggaggacagc cggcaggagt 481 ggaccttcac cctgtatgac tttgacaaca acggcaaggt cacccgagag gacatcacca 541 gcttgctgca caccatctat gaggtggtgg actcctctgt caaccactcc ccaacatcca 601 gcaagatgct gcgggtaaag ctcaccgtgg cccccgatgg cagccagagc aagaggagcg 661 tccttgtcaa tcaggctgac ctgcagagcg caaggccccg agcagagacc aagcccactg 721 aggacctgcg gagctgggag aagaagcagc gagccccgct caggttccag ggtgacagcc 781 gcctggagca gtctggctgc taccaccatt gcgtagatga gaacatcgag aggagaaacc 841 actacttaga tctcgccggg atagaaaact acacgtccca atttgggcct ggctcccctt 901 ccgtggccca gaagtcagaa ctgccccccc gcacctccaa tcccactcga tctcgctccc 961 atgagccgga agccatccac atcccacacc gaaagcccca aggcgtggac ccggcctcct 1021 tccacttcct tgacacccca atcgccaagg tctcagagct ccagcaacgg ctccggggca 1081 cccaggacgg gagcaagcac tttgtgaggt cccccaaggc ccagggcaag agtgtgggtg 1141 tgggccacgt ggccagaggg gcaagaaaca agccccctct gggacccgcc atccctgtgg 1201 tgtccccctc cgcccacctg gctgccagcc cggccctcct cccctcccta gcccccctcg 1261 ggcacaagaa gcacaagcac cgagccaagg agagccagca gggctgccgg ggcctgcagg 1321 caccactggc ctcaggtggc cctgtcctgg ggcgggagca cctgcgggag ctgcccgcct 1381 tggtggtgta tgagagccag gccgggcagc cggtccagag acatgagcac caCCACCACC 1441 ATGAACATCA CCACCATTAC CACCACTTCT ACCAGACATT GCCAACTTTC TTGTACAAAG 1501 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1561 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1621 ACGAAGGACG TCATAGCTCC TTAAATTTAC GCGTTAAGTC gacaatcaac ctctggatta 1681 caaaatttgt gaaagatt