Transcript: Human XM_017023804.1

PREDICTED: Homo sapiens NKD inhibitor of WNT signaling pathway 1 (NKD1), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
NKD1 (85407)
Length:
18635
CDS:
1968..3167

Additional Resources:

NCBI RefSeq record:
XM_017023804.1
NBCI Gene record:
NKD1 (85407)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017023804.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000422520 AGCAAGATGCTGCGGGTAAAG pLKO_005 2286 CDS 100% 10.800 15.120 N NKD1 n/a
2 TRCN0000162195 CCACTTAAACAAGCGTGGTTT pLKO.1 3935 3UTR 100% 4.950 6.930 N NKD1 n/a
3 TRCN0000160792 CCATTGCGTAGATGAGAACAT pLKO.1 2492 CDS 100% 4.950 3.960 N NKD1 n/a
4 TRCN0000164557 CGAGAGGAGAAACCACTACTT pLKO.1 2513 CDS 100% 4.950 3.960 N NKD1 n/a
5 TRCN0000418403 AGTGGACCTTCACCCTGTATG pLKO_005 2164 CDS 100% 10.800 7.560 N NKD1 n/a
6 TRCN0000160995 GAGAGGAGAAACCACTACTTA pLKO.1 2514 CDS 100% 5.625 3.938 N NKD1 n/a
7 TRCN0000164136 CCTCGAAATCTCCGAGAAGAT pLKO.1 3564 3UTR 100% 4.950 3.465 N NKD1 n/a
8 TRCN0000164309 CATCGAGAGGAGAAACCACTA pLKO.1 2510 CDS 100% 4.050 2.835 N NKD1 n/a
9 TRCN0000162714 CACCCTGTATGACTTTGACAA pLKO.1 2174 CDS 100% 4.950 2.970 N NKD1 n/a
10 TRCN0000251954 ACCATTACCACCACTTCTATC pLKO_005 3139 CDS 100% 10.800 7.560 N Nkd1 n/a
11 TRCN0000178667 CACCATTACCACCACTTCTAT pLKO.1 3138 CDS 100% 5.625 3.938 N Nkd1 n/a
12 TRCN0000434454 AGACCAACCTGGGCAACATAG pLKO_005 6752 3UTR 100% 10.800 5.400 Y SULT1A1 n/a
13 TRCN0000197864 GAAGAAGAAGAAAGAGAGAGA pLKO.1 6995 3UTR 100% 2.640 1.320 Y C230029F24Rik n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017023804.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09261 pDONR223 100% 83.6% 82.3% None (many diffs) n/a
2 TRCN0000476866 AGGACGTCATAGCTCCTTAAATTT pLX_317 3.9% 83.6% 82.3% V5 (many diffs) n/a
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