Construct: ORF TRCN0000476911

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001450.1_s317c1
Derived from:
ccsbBroadEn_05925
DNA Barcode:
CTGCCCCGCTGACCGATGTCCGAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CACNB3 (784)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476911
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 784 CACNB3 calcium voltage-gated chann... NM_000725.4 99.8% 100% 555C>A;1131C>G
2 human 784 CACNB3 calcium voltage-gated chann... NM_001206916.2 97.4% 96.5% (many diffs)
3 human 784 CACNB3 calcium voltage-gated chann... NM_001206917.2 96.9% 96.9% (many diffs)
4 human 784 CACNB3 calcium voltage-gated chann... NM_001206915.2 91.3% 91.5% 166_167ins123;432C>A;1008C>G
5 human 784 CACNB3 calcium voltage-gated chann... XM_011538727.3 63.4% 63.6% 0_1ins528;27C>A;603C>G
6 human 784 CACNB3 calcium voltage-gated chann... XM_011538728.2 63.4% 63.6% 0_1ins528;27C>A;603C>G
7 human 784 CACNB3 calcium voltage-gated chann... XM_017019957.1 63.4% 63.6% 0_1ins528;27C>A;603C>G
8 human 784 CACNB3 calcium voltage-gated chann... XM_024449180.1 63.4% 63.6% 0_1ins528;27C>A;603C>G
9 human 784 CACNB3 calcium voltage-gated chann... XM_024449181.1 63.4% 63.6% 0_1ins528;27C>A;603C>G
10 human 784 CACNB3 calcium voltage-gated chann... XR_001748868.1 53.9% (many diffs)
11 human 784 CACNB3 calcium voltage-gated chann... XR_001748867.1 52.1% (many diffs)
12 mouse 12297 Cacnb3 calcium channel, voltage-de... NM_007581.3 93.5% 99.1% (many diffs)
13 mouse 12297 Cacnb3 calcium channel, voltage-de... XM_017316415.1 92.4% 97.9% (many diffs)
14 mouse 12297 Cacnb3 calcium channel, voltage-de... XM_006520361.2 92.2% 97.7% (many diffs)
15 mouse 12297 Cacnb3 calcium channel, voltage-de... NM_001044741.2 91.2% 95.7% (many diffs)
16 mouse 12297 Cacnb3 calcium channel, voltage-de... XM_017316416.1 91% 95.5% (many diffs)
17 mouse 12297 Cacnb3 calcium channel, voltage-de... XM_017316417.1 90.7% 96% (many diffs)
18 mouse 12297 Cacnb3 calcium channel, voltage-de... XM_006520363.2 90.6% 95.5% (many diffs)
19 mouse 12297 Cacnb3 calcium channel, voltage-de... XM_006520360.1 90.1% 94.5% (many diffs)
20 mouse 12297 Cacnb3 calcium channel, voltage-de... XM_006520364.2 90% 94.8% (many diffs)
21 mouse 12297 Cacnb3 calcium channel, voltage-de... XM_006520362.3 89.9% 94.3% (many diffs)
22 mouse 12297 Cacnb3 calcium channel, voltage-de... XM_006520365.1 89.5% 94.8% (many diffs)
23 mouse 12297 Cacnb3 calcium channel, voltage-de... XM_017316418.1 89.5% 94.8% (many diffs)
24 mouse 12297 Cacnb3 calcium channel, voltage-de... NM_001286226.1 88.2% 93.5% (many diffs)
25 mouse 12297 Cacnb3 calcium channel, voltage-de... XM_006520367.1 57.8% 62.2% (many diffs)
26 mouse 12297 Cacnb3 calcium channel, voltage-de... XM_017316419.1 57.8% 62.2% (many diffs)
27 mouse 12297 Cacnb3 calcium channel, voltage-de... XM_006520370.1 44.5% 47.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1518
ORF length:
1452
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgta tgacgactcc tacgtgcccg ggtttgagga ctcggaggcg ggttcagccg
121actcctacac cagccgccca tctctggact cagacgtctc cctggaggag gaccgggaga
181gtgcccggcg tgaagtagag agccaggctc agcagcagct cgaaagggcc aagcacaaac
241ctgtggcatt tgcggtgagg accaatgtca gctactgtgg cgtactggat gaggagtgcc
301cagtccaggg ctctggagtc aactttgagg ccaaagattt tctgcacatt aaagagaagt
361acagcaatga ctggtggatc gggcggctag tgaaagaggg cggggacatc gccttcatcc
421ccagccccca gcgcctggag agcatccggc tcaaacagga gcagaaggcc aggagatctg
481ggaacccttc cagcctgagt gacattggca accgacgctc ccctccgcca tctctagcca
541agcagaagca aaagcaggcg gaacatgttc ccccatatga cgtggtgccc tccatgcggc
601ctgtggtgct ggtgggacca tctctgaaag gttatgaggt cacagacatg atgcagaagg
661ctctcttcga cttcctcaaa cacagatttg atggcaggat ctccatcacc cgagtcacag
721ccgacctctc cctggcaaag cgatctgtgc tcaacaatcc gggcaagagg accatcattg
781agcgctcctc tgcccgctcc agcattgcgg aagtgcagag tgagatcgag cgcatatttg
841agctggccaa atccctgcag ctagtagtgt tggacgctga caccatcaac cacccagcac
901agctggccaa gacctcgctg gcccccatca tcgtctttgt caaagtgtcc tcaccaaagg
961tactccagcg tctcattcgc tcccggggga agtcacagat gaagcacctg accgtacaga
1021tgatggcata tgataagctg gttcagtgcc caccggagtc atttgatgtg attctggatg
1081agaaccagct ggaggatgcc tgtgagcacc tggctgagta cctggaggtt tactggcggg
1141ccacgcacca cccagcccct ggccccggac ttctgggtcc TCCCAGTGCC ATCCCGGGAC
1201TTCAGAACCA GCAGCTGCTG GGGGAGCGTG GCGAGGAGCA CTCCCCCCTT GAGCGGGACA
1261GCTTGATGCC CTCTGATGAG GCCAGCGAGA GCTCCCGCCA AGCCTGGACA GGATCTTCAC
1321AGCGTAGCTC CCGCCACCTG GAGGAGGACT ATGCAGATGC CTACCAGGAC CTGTACCAGC
1381CTCACCGCCA ACACACCTCG GGGCTGCCTA GTGCTAACGG GCATGACCCC CAAGACCGGC
1441TTCTAGCCCA GGACTCAGAG CACAACCACA GTGACCGGAA CTGGCAGCGC AACCGGCCTT
1501GGCCCAAGGA TAGCTACTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA
1561TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA
1621GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACTGCCCC GCTGACCGAT
1681GTCCGATACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)