Construct: ORF TRCN0000476916
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011692.1_s317c1
- Derived from:
- ccsbBroadEn_00230
- DNA Barcode:
- CACTGAACTGGTGGTGTTCGACGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RUNX3 (864)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476916
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 864 | RUNX3 | RUNX family transcription f... | NM_001031680.2 | 100% | 100% | |
| 2 | human | 864 | RUNX3 | RUNX family transcription f... | NM_001320672.1 | 100% | 100% | |
| 3 | human | 864 | RUNX3 | RUNX family transcription f... | XM_005246024.4 | 100% | 100% | |
| 4 | human | 864 | RUNX3 | RUNX family transcription f... | NM_004350.3 | 96.6% | 95.1% | (many diffs) |
| 5 | human | 864 | RUNX3 | RUNX family transcription f... | XM_011542351.1 | 87.6% | 87.6% | 586_587ins159 |
| 6 | mouse | 12399 | Runx3 | runt related transcription ... | NM_019732.2 | 88% | 91.3% | (many diffs) |
| 7 | mouse | 12399 | Runx3 | runt related transcription ... | XM_006538506.3 | 62.1% | 64.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1353
- ORF length:
- 1287
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc atcgaacagc atcttcgact ccttcccgac ctactcgccg accttcatcc 121 gcgacccaag caccagccgc cgcttcacac ctccctcccc ggccttcccc tgcggcggcg 181 gcggcggcaa gatgggcgag aacagcggcg cgctgagcgc gcaggcggcc gtggggcccg 241 gagggcgcgc ccggcccgag gtgcgctcga tggtggacgt gctggcggac cacgcaggcg 301 agctcgtgcg caccgacagc cccaacttcc tctgctccgt gctgccctcg cactggcgct 361 gcaacaagac gctgcccgtc gccttcaagg tggtggcatt gggggacgtg ccggatggta 421 cggtggtgac tgtgatggca ggcaatgacg agaactactc cgctgagctg cgcaatgcct 481 cggccgtcat gaagaaccag gtggccaggt tcaacgacct tcgcttcgtg ggccgcagtg 541 ggcgagggaa gagtttcacc ctgaccatca ctgtgttcac caaccccacc caagtggcga 601 cctaccaccg agccatcaag gtgaccgtgg acggaccccg ggagcccaga cggcaccggc 661 agaagctgga ggaccagacc aagccgttcc ctgaccgctt tggggacctg gaacggctgc 721 gcatgcgggt gacaccgagc acacccagcc cccgaggctc actcagcacc acaagccact 781 tcagcagcca gccccagacc ccaatccaag gcacctcgga actgaaccca ttctccgacc 841 cccgccagtt tgaccgctcc ttccccacgc tgccaaccct cacggagagc cgcttcccag 901 accccaggat gcattatccc ggggccatgt cagctgcctt cccctacagc gccacgccct 961 cgggcacgag catcagcagc ctcagcgtgg cgggcatgcc ggccaccagc cgcttccacc 1021 atacctacct cccgccaccc tacccggggg ccccgcagaa ccagagcggg cccttccagg 1081 ccaacccgtc cccctaccac ctctactacg ggacaTCCTC TGGCTCCTAC CAGTTCTCCA 1141 TGGTGGCCGG CAGCAGCAGT GGGGGCGACC GCTCACCTAC CCGCATGCTG GCCTCTTGCA 1201 CCAGCAGCGC TGCCTCTGTC GCCGCCGGCA ACCTCATGAA CCCCAGCCTG GGCGGCCAGA 1261 GTGATGGCGT GGAGGCCGAC GGCAGCCACA GCAACTCACC CACGGCCCTG AGCACGCCAG 1321 GCCGCATGGA TGAGGCCGTG TGGCGGCCCT ACTGCCCAAC TTTCTTGTAC AAAGTGGTTG 1381 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1441 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACA 1501 CTGAACTGGT GGTGTTCGAC GCACGCGTTA AGTCgacaat caacctctgg attacaaaat 1561 ttgtgaaaga tt