Construct: ORF TRCN0000476981
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007133.1_s317c1
- Derived from:
- ccsbBroadEn_03139
- DNA Barcode:
- CAGATTCGAGGCCTCGGCGCCATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KLK14 (43847)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476981
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 43847 | KLK14 | kallikrein related peptidas... | NM_001311182.2 | 94% | 94% | 0_1ins48 |
| 2 | human | 43847 | KLK14 | kallikrein related peptidas... | NM_001369775.2 | 94% | 94% | 0_1ins48 |
| 3 | human | 43847 | KLK14 | kallikrein related peptidas... | NM_022046.6 | 94% | 94% | 0_1ins48 |
| 4 | human | 43847 | KLK14 | kallikrein related peptidas... | XM_017026820.1 | 67% | 64.4% | 513_514insGCCA;537_538ins260 |
| 5 | human | 43847 | KLK14 | kallikrein related peptidas... | NR_163144.1 | 57.8% | 1_109del;369_370ins254;657_692del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 870
- ORF length:
- 801
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtccctgagg gtcttgggct ctgggacctg gccctcagcc cctaaaatgt 121 tcctcctgct gacagcactt caagtcctgg ctatagccat gacacagagc caagaggatg 181 agaacaagat aattggtggc catacgtgca cccggagctc ccagccgtgg caggcggccc 241 tgctggcggg tcccaggcgc cgcttcctct gcggaggcgc cctgctttca ggccagtggg 301 tcatcactgc tgctcactgc ggccgcccga tccttcaggt tgccctgggc aagcacaacc 361 tgaggaggtg ggaggccacc cagcaggtgc tgcgcgtggt tcgtcaggtg acgcacccca 421 actacaactc ccggacccac gacaacgacc tcatgctgct gcagctacag cagcccgcac 481 ggatcgggag ggcagtcagg cccattgagg TCACCCAGGC CTGTGCCAGC CCCGGGACCT 541 CCTGCCGAGT GTCAGGCTGG GGAACTATAT CCAGCCCCAT CGCCAGGTAC CCCGCCTCTC 601 TGCAATGCGT GAACATCAAC ATCTCCCCGG ATGAGGTGTG CCAGAAGGCC TATCCTAGAA 661 CCATCACGCC TGGCATGGTC TGTGCAGGAG TTCCCCAGGG CGGGAAGGAC TCTTGTCAGG 721 GTGACTCTGG GGGACCCCTG GTGTGCAGAG GACAGCTCCA GGGCCTCGTG TCTTGGGGAA 781 TGGAGCGCTG CGCCCTGCCT GGCTACCCCG GTGTCTACAC CAACCTGTGC AAGTACAGAA 841 GCTGGATTGA GGAAACGATG CGGGACAAAT TGCCAACTTT CTTGTACAAA GTGGTTGATA 901 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 961 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACAGAT 1021 TCGAGGCCTC GGCGCCATAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1081 tgaaagatt