Construct: ORF TRCN0000476985
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004078.1_s317c1
- Derived from:
- ccsbBroadEn_05946
- DNA Barcode:
- AGTCCTGTTTTAACGAGAATCACA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- CCNA2 (890)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476985
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 890 | CCNA2 | cyclin A2 | NM_001237.5 | 99.8% | 2.7% | 37G>T;487A>G |
| 2 | mouse | 12428 | Ccna2 | cyclin A2 | NM_009828.2 | 83.8% | .7% | (many diffs) |
| 3 | mouse | 12428 | Ccna2 | cyclin A2 | XM_017319444.1 | 83.8% | .7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 105
- ORF length:
- 36
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gttgggcaac tctgcgccgg ggcctgcgac ccgctaggcg ggctcggcgc 121 tgctagcatt gcagcagacg gcgctccaag aggaccagga gaatatcaac ccggaaaagg 181 cagcgcccgt ccaacaaccg cggacccggg ccgcgctggc ggtactgaag tccgggaacc 241 cgcggggtct agcgcagcag cagaggccga agacgagacg ggttgcaccc cttaaggatc 301 ttcctgtaaa tgatgagcat gtcaccgttc ctccttggaa agcaaacagt aaacagcctg 361 cgttcaccat tcatgtggat gaagcagaaa aagaagctca gaagaagcca gctgaatctc 421 aaaaaataga gcgtgaagat gccctggctt ttaattcagc cattagttta cctggaccca 481 gaaaaccatt ggtccctctt gattatccaa tggatggtag ttttgagtca ccacatacta 541 tggacatgtc aattgtatta gaagatgaaa agccagtgag tgttaatgaa gtaccagact 601 accatgagga tattcacaca taccttaggg aaatggaggt taaatgtaaa cctaaagtgg 661 gttacatgaa gaaacagcca gacatcacta acagtatgag agctatcctc gtggactggt 721 tagttgaagt aggagaagaa tataaactac agaatgagac cctgcatttg gctgtgaact 781 acattgatag gttcctgtct tccatgtcag tgctgagagg aaaacttcag cttgtgggca 841 ctgctgctat gctgttagcc tcaaagtttg aagaaatata ccccccagaa gtagcagagt 901 ttgtgtacat tacagatgat acctacacca agaaacaagt tctgagaatg gagcatctag 961 ttttgaaagt ccttactttt gacttagctg ctccaacagt aaatcagttt cttacccaat 1021 actttctgca tcagcagcct gcaaactgca aagttgaaag tttagcaatg tttttgggag 1081 aattaagttt gatagatgct gacccatacc tcaagtattt gccatcagtt attgctGGAG 1141 CTGCCTTTCA TTTAGCACTC TACACAGTCA CGGGACAAAG CTGGCCTGAA TCATTAATAC 1201 GAAAGACTGG ATATACCCTG GAAAGTCTTA AGCCTTGTCT CATGGACCTT CACCAGACCT 1261 ACCTCAAAGC ACCACAGCAT GCACAACAGT CAATAAGAGA AAAGTACAAA AATTCAAAGT 1321 ATCATGGTGT TTCTCTCCTC AACCCACCAG AGACACTAAA TCTGTTGCCA ACTTTCTTGT 1381 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1441 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1501 GAAAGGACGA AGTCCTGTTT TAACGAGAAT CACAACGCGT TAAGTCgaca atcaacctct 1561 ggattacaaa atttgtgaaa gatt