Construct: ORF TRCN0000476995
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007021.1_s317c1
- Derived from:
- ccsbBroadEn_10134
- DNA Barcode:
- GAGTAAGATCGCAGGCCACGAGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GOLGA7B (401647)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476995
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 401647 | GOLGA7B | golgin A7 family member B | NM_001010917.3 | 99.8% | 99.4% | 248C>T |
| 2 | mouse | 71146 | Golga7b | golgi autoantigen, golgin s... | NM_001141983.1 | 91% | 98.2% | (many diffs) |
| 3 | mouse | 71146 | Golga7b | golgi autoantigen, golgin s... | NM_027694.2 | 91% | 98.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 570
- ORF length:
- 501
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccaccgag gtccacaatc tgcaggagct ccggcgaagt gcctcactgg 121 ccaccaaggt ctttatccag agagactaca gcgatgggac catctgtcag ttccagacca 181 aattcccccc agagctggac agccggatcg agcggcagct ctttgaagag actgtgaaga 241 ccctcaacgg attttacgca gaggctgaga agattggggg cagctcctac ctcgagggct 301 gcctggccTG CGCCATGGCC TACTTCATCT TCCTCTGCAT GGAGACCCAC TATGAGAAGG 361 TTCTCAAGAA GATTTCCCGC TACATCCAGG AGCAGAATGA GAAGATCTTT GCACCTCGAG 421 GCCTCCTACT TACAGACCCT GTGGAGCGTG GGATGAGGGT TATTGAGATC TCCATCTACG 481 AGGACCGGTG CAGCAGTGGC AGCTCCAGCA GCGGCAGCAG CAGCGGCAGT GGCAGCAGCA 541 GCGGTGGGGG TGGTGGGGCG GGGGCCCGGT TGCCAACTTT CTTGTACAAA GTGGTTGATA 601 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 661 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGAGTA 721 AGATCGCAGG CCACGAGGAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 781 tgaaagatt