Transcript: Human NM_001010917.3

Homo sapiens golgin A7 family member B (GOLGA7B), mRNA.

Source:
NCBI, updated 2019-05-01
Taxon:
Homo sapiens (human)
Gene:
GOLGA7B (401647)
Length:
6843
CDS:
462..965

Additional Resources:

NCBI RefSeq record:
NM_001010917.3
NBCI Gene record:
GOLGA7B (401647)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001010917.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000140623 GTGAAGACCCTCAACGGATTT pLKO.1 627 CDS 100% 10.800 7.560 N GOLGA7B n/a
2 TRCN0000122793 GCCAGTTTCCTGAGATTAGAA pLKO.1 1748 3UTR 100% 5.625 3.938 N GOLGA7B n/a
3 TRCN0000143050 CAAGAAGATTTCCCGCTACAT pLKO.1 758 CDS 100% 4.950 3.465 N GOLGA7B n/a
4 TRCN0000143401 CCAGTATCTGTTCCTTCTCAT pLKO.1 1421 3UTR 100% 4.950 3.465 N GOLGA7B n/a
5 TRCN0000121898 CCCTGTAAGTAAATGTCCAAA pLKO.1 2213 3UTR 100% 4.950 3.465 N GOLGA7B n/a
6 TRCN0000143457 GCTCTTTGAAGAGACTGTGAA pLKO.1 611 CDS 100% 4.950 3.465 N GOLGA7B n/a
7 TRCN0000140197 GAGACCCACTATGAGAAGGTT pLKO.1 735 CDS 100% 3.000 2.100 N GOLGA7B n/a
8 TRCN0000143049 CAAGGTCTTTATCCAGAGAGA pLKO.1 518 CDS 100% 2.640 1.848 N GOLGA7B n/a
9 TRCN0000139701 CATCTGTCAGTTCCAGACCAA pLKO.1 554 CDS 100% 2.640 1.848 N GOLGA7B n/a
10 TRCN0000140198 GAGAAGATCTTTGCACCTCGA pLKO.1 792 CDS 100% 2.160 1.512 N GOLGA7B n/a
11 TRCN0000143402 CCACTATGAGAAGGTTCTCAA pLKO.1 740 CDS 100% 0.495 0.347 N GOLGA7B n/a
12 TRCN0000144947 GAGCAGAATGAGAAGATCTTT pLKO.1 783 CDS 100% 5.625 2.813 Y GOLGA7B n/a
13 TRCN0000134748 GAGCAGAATGAGAAGATCTAT pLKO.1 783 CDS 100% 5.625 2.813 Y GOLGA7 n/a
14 TRCN0000343302 GAGCAGAATGAGAAGATCTAT pLKO_005 783 CDS 100% 5.625 2.813 Y GOLGA7 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001010917.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10134 pDONR223 100% 99.8% 99.4% None 248C>T n/a
2 ccsbBroad304_10134 pLX_304 0% 99.8% 99.4% V5 248C>T n/a
3 TRCN0000476995 GAGTAAGATCGCAGGCCACGAGGA pLX_317 51.9% 99.8% 99.4% V5 248C>T n/a
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