Transcript: Mouse NM_001141983.1

Mus musculus golgi autoantigen, golgin subfamily a, 7B (Golga7b), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-05-21
Taxon:
Mus musculus (mouse)
Gene:
Golga7b (71146)
Length:
2839
CDS:
443..946

Additional Resources:

NCBI RefSeq record:
NM_001141983.1
NBCI Gene record:
Golga7b (71146)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001141983.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000192102 CAAGAAGATTTCCCGTTACAT pLKO.1 739 CDS 100% 5.625 7.875 N Golga7b n/a
2 TRCN0000190619 GCATGATACTTCACCCTCAAA pLKO.1 1313 3UTR 100% 4.950 6.930 N Golga7b n/a
3 TRCN0000202013 CGGAATGAGGGTTATCGAGAT pLKO.1 823 CDS 100% 4.050 5.670 N Golga7b n/a
4 TRCN0000201498 CAGAATGAGAAGGTCTTTGCT pLKO.1 767 CDS 100% 3.000 2.100 N Golga7b n/a
5 TRCN0000140197 GAGACCCACTATGAGAAGGTT pLKO.1 716 CDS 100% 3.000 2.100 N GOLGA7B n/a
6 TRCN0000143049 CAAGGTCTTTATCCAGAGAGA pLKO.1 499 CDS 100% 2.640 1.848 N GOLGA7B n/a
7 TRCN0000139701 CATCTGTCAGTTCCAGACCAA pLKO.1 535 CDS 100% 2.640 1.848 N GOLGA7B n/a
8 TRCN0000143402 CCACTATGAGAAGGTTCTCAA pLKO.1 721 CDS 100% 0.495 0.347 N GOLGA7B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001141983.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10134 pDONR223 100% 91% 98.2% None (many diffs) n/a
2 ccsbBroad304_10134 pLX_304 0% 91% 98.2% V5 (many diffs) n/a
3 TRCN0000476995 GAGTAAGATCGCAGGCCACGAGGA pLX_317 51.9% 91% 98.2% V5 (many diffs) n/a
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