Construct: ORF TRCN0000477039
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008056.1_s317c1
- Derived from:
- ccsbBroadEn_00623
- DNA Barcode:
- ATCTGACCAGGGTTATGGTGAGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GCHFR (2644)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477039
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2644 | GCHFR | GTP cyclohydrolase I feedba... | NM_005258.3 | 100% | 100% | |
| 2 | mouse | 320415 | Gchfr | GTP cyclohydrolase I feedba... | NM_177157.4 | 91.6% | 92.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 321
- ORF length:
- 252
- Sequence:
-
1 TCTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCACCAT GCCCTACCTG CTCATCAGCA CCCAGATCCG CATGGAGGTG GGCCCCACTA 121 TGGTGGGCGA TGAACAGTCG GATCCAGAGC TGATGCAGCA TCTGGGGGCT TCAAAGAGAA 181 GAGCCTTGGG AAACAACTTT TATGAATACT ACGTCGATGA CCCTCCCCGC ATAGTCCTGG 241 ACAAGCTGGA ACGCAGGGGC TTCCGTGTGC TGAGCATGAC GGGGGTGGGC CAGACGCTGG 301 TGTGGTGTCT GCACAAGGAG TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 361 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 421 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAATCT GACCAGGGTT 481 ATGGTGAGTT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt