Construct: ORF TRCN0000477052
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015615.1_s317c1
- Derived from:
- ccsbBroadEn_08414
- DNA Barcode:
- CCGCACTTGCACGAGTTAATTTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SWT1 (54823)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477052
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54823 | SWT1 | SWT1 RNA endoribonuclease h... | NM_001105518.1 | 99.7% | 99.5% | (many diffs) |
| 2 | human | 54823 | SWT1 | SWT1 RNA endoribonuclease h... | NM_017673.7 | 99.7% | 99.5% | (many diffs) |
| 3 | human | 54823 | SWT1 | SWT1 RNA endoribonuclease h... | XM_017001527.1 | 99.7% | 99.5% | (many diffs) |
| 4 | human | 54823 | SWT1 | SWT1 RNA endoribonuclease h... | XM_024447754.1 | 99.7% | 99.5% | (many diffs) |
| 5 | human | 54823 | SWT1 | SWT1 RNA endoribonuclease h... | XM_005245271.2 | 98.9% | 98.6% | (many diffs) |
| 6 | human | 54823 | SWT1 | SWT1 RNA endoribonuclease h... | XM_005245273.1 | 98.9% | 98.6% | (many diffs) |
| 7 | human | 54823 | SWT1 | SWT1 RNA endoribonuclease h... | XM_011509657.1 | 98.9% | 98.6% | (many diffs) |
| 8 | human | 54823 | SWT1 | SWT1 RNA endoribonuclease h... | XM_024447752.1 | 98.9% | 98.6% | (many diffs) |
| 9 | human | 54823 | SWT1 | SWT1 RNA endoribonuclease h... | XM_024447753.1 | 98.9% | 98.6% | (many diffs) |
| 10 | human | 54823 | SWT1 | SWT1 RNA endoribonuclease h... | XM_017001529.1 | 91.5% | 90.4% | (many diffs) |
| 11 | human | 54823 | SWT1 | SWT1 RNA endoribonuclease h... | XM_017001530.2 | 90.6% | 88.3% | (many diffs) |
| 12 | human | 54823 | SWT1 | SWT1 RNA endoribonuclease h... | XM_017001528.2 | 87.9% | 89.1% | (many diffs) |
| 13 | human | 54823 | SWT1 | SWT1 RNA endoribonuclease h... | XR_001737246.2 | 68.9% | (many diffs) | |
| 14 | mouse | 66875 | Swt1 | SWT1 RNA endoribonuclease h... | XM_011248059.1 | 49.3% | 46% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2766
- ORF length:
- 2700
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc cagcaaagaa tcctgtggga aaaaagagac atctcagagg aaagacacca 121 ccacctcatc acccaatttt ggtgaaaaag acaagaaaga gagaaaaacc ccagcaagtt 181 ctactagttc gtcttctata agatcagttt catcagaaaa gagaaaactg aaatcagatc 241 atacagatgt tctgtactat aatataaaaa gaagacaagg actgaaaaga ttgagtgtag 301 aaattgacac tctcagaagg agaccaaaaa tcggttcttc atcccaaaga cctattaaac 361 tcaaagaagc atcatattca aatgataatc aaattatttt gcagagtcct tcttcaaatg 421 gaactaaaaa agacatacat aaatgtgtag actttaaacc taaagatatc aaattgacaa 481 atgctgggag caagcttgac catggagtta aaagccttag tagtcctaag attgccagtg 541 atgtgaaacc taaagccgaa ggccaggcaa gtgaaaataa atggtctcat ttacttgttc 601 agagagagaa gatgaaagaa ctcaagaaag gaagaaacag taaatttaga gacaattctg 661 aaaaatgtgt cttagagaaa tggaagagaa atcaattttc tcaggattat aactccaaca 721 agataattaa ggaacccttg ggatctagga gacagaagat cagtttcaaa atccctataa 781 aatcccgtga caccctccag aaacttgtag aagaaaatgt cttcaacata gattctaata 841 attcgaagac taagcaggaa gaaagagaat acctggaaag ctcccaggtt tcattaaatg 901 tgactaggca gaaaactgaa catttacttt cagattttac atataagcgg actgttcatg 961 agtggaaacg aaaacatcat tatgaccatc aagaaagtaa tgattcacat tctagggaaa 1021 acctaaccca gagttttgaa gcaccatgtt gttccgtgtc atctgaaagt atccaggatg 1081 cagatcaaga gatgcagata gtagaagagc ttcatgctgc acgtgtggga aaaagtgtgg 1141 atttacctgg agagttaatg agtatggaaa ttgacttaga agatgatgta cattcctcct 1201 ctgcaaataa tacttcagac agaaagcttc taattgttat tgacacaaat attctgatga 1261 atcatctcaa atttgttaga attttgaaga caacagaagt accaggtttt gacaaacttg 1321 tgttaataat tccctgggtc gttatgcaag agctagatcg tatgaaggaa ggaaaactac 1381 taaaacgtgc ccagcacaaa gctatacctg cagttcattt catcaacgac agtctcaaaa 1441 atcaagatag aaagctatgg ggtcagtcaa tacaacttgc atcccaaaaa cattatggat 1501 tgagtgatga gaacaatgat gatcgagtac taaaatgctg tctccagcac caggaattat 1561 tcccttgttc ttttgttatt ctgtgcacgg atgatagaaa cttaagaaac aaaggcctga 1621 taagtggtgt gaagtcactc agtaaagaag aattgagtgc agagttatta cgcttatctc 1681 tgaacacaga tgtgtgtcat cagccttgta ttcctaagca acagttgaaa gcagaaacaa 1741 cacccttgaa agagagctat aaggaggaat ctacaagttc tggactgtcc attctgcttg 1801 agagcattgt atctgatctt gaaaaatctc ttggaacagg tttatcttca atattagaaa 1861 cagaaatgaa aattgctttt ggaaaccttt ggatggagat cctgtacctg aaaccaccat 1921 ggactctact acatttacta cagtgcttta aaaaacattg gttggctgta tttggattag 1981 ttatggaaaa gaacttgctt ttaactattg agagcctata caaaaatctc cgtaaagcta 2041 ataaggcagt ggattttaca acagtcaaat tcttgcttca ggattctaga agtttgttac 2101 atgctttcag tacaaggtca aattatgatg gtattcttcc acagaccttt gctcaagtaa 2161 acaacctcct tcagacattt gcagaggtca agacaaaact taagccaaat tcttcagaaa 2221 acacagtgac taaaaagcag gaaggtactt cattgaagaa ttctcataat caagaaatca 2281 ctgttttctc gagttctcat cttccccaac ccagcaggca tcaagaaatc tggtctaTCC 2341 TAGAGAGTGT TTGGATTACA ATATATCAGA ACAGCACGGA TGTATTTCAA AGATTGGGCT 2401 CAAATTCAGC TCTGACTACT TCAAATATAG CATCATTTGA AGAAGCATTT ATATGTCTTC 2461 AAAAGTTAAT GGCAGCTGTG AGGGATATTC TTGAAGGAAT TCAAAGGATT TTGGCCCCAA 2521 ACAGTGATTA TCAAGATGTT GAGACCCTCT ATAACTTCCT AATCAAGTAT GAGGTAAATA 2581 AAAATGTCAA ATTTACTGCC CAGGAAATTT ATGATTGTGT TTCTCAGACT GAGTATAGGG 2641 AAAAGTTAAC CATTGGATGC CGCCAGCTGG TTGAGATGGA ATATACCATG CAGCAGTGCA 2701 ATGCATCTGT TTATATGGAG GCCAAAAACA GGGGATGGTG TGAAGACATG CTCAACTATA 2761 GGATATACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2821 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 2881 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACCGCACTTG CACGAGTTAA TTTTTACGCG 2941 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt