Construct: ORF TRCN0000477055
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000390.1_s317c1
- Derived from:
- ccsbBroadEn_07475
- DNA Barcode:
- CGTTTGGGGATGGAGCACACGGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEMA3E (9723)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477055
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9723 | SEMA3E | semaphorin 3E | NM_012431.3 | 99.9% | 100% | 1497G>A |
| 2 | human | 9723 | SEMA3E | semaphorin 3E | NM_001178129.1 | 92.2% | 92.2% | 0_1ins180;1317G>A |
| 3 | mouse | 20349 | Sema3e | sema domain, immunoglobulin... | NM_011348.2 | 85% | 90.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2394
- ORF length:
- 2325
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcatccgcg gggcacatta tcaccttgct cctgtggggt tacttactgg 121 agctttggac aggaggtcat acagctgata ctacccaccc ccggttacgc ctgtcacata 181 aagagctctt gaatctgaac agaacatcaa tatttcatag cccttttgga tttcttgatc 241 tccatacaat gctgctggat gaatatcaag agaggctctt cgtgggaggc agggaccttg 301 tatattccct cagcttggag agaatcagtg acggctataa agagatacac tggccgagta 361 cagctctaaa aatggaagaa tgcataatga agggaaaaga tgcgggtgaa tgtgcaaatt 421 atgttcgggt tttgcatcac tataacagga cacaccttct gacctgtggt actggagctt 481 ttgatccagt ttgtgccttc atcagagttg gatatcattt ggaggatcct ctgtttcacc 541 tggaatcacc cagatctgag agaggaaggg gcagatgtcc ttttgacccc agctcctcct 601 tcatctccac tttaattggt agtgaattgt ttgctggact ctacagtgac tactggagca 661 gagacgctgc gatcttccgc agcatggggc gactggccca tatccgcact gagcatgacg 721 atgagcgtct gttgaaagaa ccaaaatttg taggttcata catgattcct gacaatgaag 781 acagagatga caacaaagta tatttctttt ttactgagaa ggcactggag gcagaaaaca 841 atgctcacgc aatttacacc agggtcgggc gactctgtgt gaatgatgta ggagggcaga 901 gaatactggt gaataagtgg agcactttcc taaaagcgag actcgtttgc tcagtaccag 961 gaatgaatgg aattgacaca tattttgatg aattagagga cgtttttttg ctacctacca 1021 gagatcataa gaatccagtg atatttggac tctttaacac taccagtaat atttttcgag 1081 ggcatgctat atgtgtctat cacatgtcta gcattcgggc agccttcaac ggaccatatg 1141 cacataagga aggacctgaa taccactggt cagtctatga aggaaaagtc ccttatccaa 1201 ggcctggttc ttgtgccagc aaagtaaatg gagggagata cggaaccacc aaggactatc 1261 ctgatgatgc catccgattt gcaagaagtc atccactaat gtaccaggcc ataaaacctg 1321 cccataaaaa accaatattg gtaaaaacag atggaaaata taacctgaaa caaatagcag 1381 tagatcgagt ggaagctgag gatggccaat atgacgtctt gtttattggg acagataatg 1441 gaattgtgct gaaagtaatc acaatttaca accaagaaat ggaatcaatg gaagaagtaa 1501 ttctagaaga acttcagata ttcaaggatc cagttcctat tatttctatg gagatttctt 1561 caaaacggca acagctgtat attggatctg cttctgctgt ggctcaagtc agattccatc 1621 actgtgacat gtatggaagt gcttgtgctg actgctgcct ggctcgagac ccttactgtg 1681 cctgggatgg catatcctgc tcccggtatt acccaacagg cacacatgca aaaaggcgtt 1741 tccggagaca agatgttcga catggaaatg cagctcagca gtgctttgga caacagtttg 1801 ttggggatgc tttggataag actgaagaac atctggctta tggcatagag aacaacagta 1861 ctttgctgga atgtacccca cgatctttac aagcgaaagt tatctggttt gtacagaaag 1921 gacgtgagac aagaaaagag gaggtgaaga cagatgacag agtggttaag atggaccttg 1981 gtttactctt cctaaggtta cacaaatcag atgctgggac ctatttttgc cagacagtag 2041 agcatagctt tgtccatacg gtccgtaaaa tcaccttGGA GGTAGTGGAA GAGGAGAAAG 2101 TCGAGGATAT GTTTAACAAG GACGATGAGG AGGACAGGCA TCACAGGATG CCTTGTCCTG 2161 CTCAGAGTAG CATCTCGCAG GGAGCAAAAC CATGGTACAA GGAATTCTTG CAGCTGATCG 2221 GTTATAGCAA CTTCCAGAGA GTGGAAGAAT ACTGCGAGAA AGTATGGTGC ACAGATAGAA 2281 AGAGGAAAAA GCTTAAAATG TCACCCTCCA AGTGGAAGTA TGCCAACCCT CAGGAAAAGA 2341 AGCTCCGTTC CAAACCTGAG CATTACCGCC TGCCCAGGCA CACGCTGGAC TCCTTGCCAA 2401 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 2461 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAAGTA TTTCGATTTC TTGGCTTTAT 2521 ATATCTTGTG GAAAGGACGA CGTTTGGGGA TGGAGCACAC GGTCACGCGT TAAGTCgaca 2581 atcaacctct ggattacaaa atttgtgaaa gatt