Transcript: Mouse NM_011348.2

Mus musculus sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E (Sema3e), mRNA.

Source:
NCBI, updated 2017-06-03
Taxon:
Mus musculus (mouse)
Gene:
Sema3e (20349)
Length:
6877
CDS:
635..2962

Additional Resources:

NCBI RefSeq record:
NM_011348.2
NBCI Gene record:
Sema3e (20349)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_011348.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000112296 CCAGTGATATTTGGACTGTTT pLKO.1 1601 CDS 100% 4.950 3.465 N Sema3e n/a
2 TRCN0000112295 CCATCCATTCTGAGGCTTGTT pLKO.1 3512 3UTR 100% 4.950 2.970 N Sema3e n/a
3 TRCN0000112298 CCTTGTCTATTCCCTGAACTT pLKO.1 862 CDS 100% 4.950 2.970 N Sema3e n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_011348.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07475 pDONR223 100% 85% 90.1% None (many diffs) n/a
2 ccsbBroad304_07475 pLX_304 0% 85% 90.1% V5 (many diffs) n/a
3 TRCN0000477055 CGTTTGGGGATGGAGCACACGGTC pLX_317 13.9% 85% 90.1% V5 (many diffs) n/a
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