Construct: ORF TRCN0000477129
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002023.1_s317c1
- Derived from:
- ccsbBroadEn_03116
- DNA Barcode:
- GCTTAACCAACCCTCTTTTCAAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRHDE (29953)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477129
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29953 | TRHDE | thyrotropin releasing hormo... | NM_013381.2 | 100% | 100% | |
| 2 | human | 29953 | TRHDE | thyrotropin releasing hormo... | XM_017019243.2 | 94.4% | 92.4% | (many diffs) |
| 3 | human | 29953 | TRHDE | thyrotropin releasing hormo... | XM_005268819.5 | 71.4% | 71.1% | (many diffs) |
| 4 | human | 29953 | TRHDE | thyrotropin releasing hormo... | XM_017019244.1 | 70.4% | 70.4% | 0_1ins909 |
| 5 | human | 29953 | TRHDE | thyrotropin releasing hormo... | XM_011538248.2 | 60.4% | 60.4% | 0_1ins1215 |
| 6 | mouse | 237553 | Trhde | TRH-degrading enzyme | NM_146241.2 | 90% | 95.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3141
- ORF length:
- 3072
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggggaagac gacgccgcgc ttcgggctgg cagcaggggg ctctccgacc 121 cgtgggcaga ctcagtggga gtgcgacccc gcaccacgga gcgccacatc gccgtacaca 181 agcggcttgt gctggccttc gctgtgtccc tcgtggcatt gctcgcggtc acaatgctcg 241 ctgtgctgct cagcctgcgc ttcgacgagt gcggggcgag tgccacgcca ggcgccgacg 301 gtggcccctc aggctttccg gagcgcggcg gcaacgggag cctccctgga tcggcccggc 361 gcaaccacca cgcaggcggg gactcctggc agcccgaggc gggtggggtg gccagtccgg 421 ggaccacgtc ggcccagccg ccgtcggagg aggagcggga gccgtgggag ccgtggacgc 481 agctgcgcct gtcgggccac ctgaagccgc tgcactacaa tctgatgctc accgccttca 541 tggagaactt caccttctcc ggggaggtca acgtggagat cgcgtgccgg aacgccaccc 601 gctacgtagt gctgcacgct tcccgagtgg cggtggagaa agtgcagctg gccgaggacc 661 gggcgttcgg ggctgtccct gtagccggtt ttttcctcta cccgcaaacc caggtcttag 721 tggtggtgct gaataggaca ctggacgcgc agaggaatta caatctgaag attatctaca 781 acgcgctcat cgagaatgag ctcctgggct tcttccgcag ctcctatgtg ctccacgggg 841 agagaagatt ccttggtgtt actcagtttt cgcctacaca tgccagaaag gcatttcctt 901 gttttgatga gccaatctac aaggctactt tcaaaatcag catcaagcat caagcaacct 961 atttatcttt atctaatatg ccagtggaaa cttccgtgtt tgaggaagat ggatgggtta 1021 cggatcactt ttcacagacc cctctcatgt ccacatatta tttagcctgg gcaatttgca 1081 acttcacata cagagaaact accaccaaga gtggggttgt agtacgatta tatgcaagac 1141 ctgatgctat cagaagagga tccggggact atgctctcca tataacaaag agattaatag 1201 aattttatga agactacttt aaagtgccct attccttgcc aaaactagat cttttagctg 1261 tgcctaagca tccgtatgct gctatggaga actggggact aagtattttt gtggaacaaa 1321 gaatactgct ggatcccagt gtttcatcta tttcttattt gctggatgtc accatggtca 1381 ttgttcatga gatatgtcac cagtggtttg gtgaccttgt gacgcctgtg tggtgggaag 1441 acgtgtggct gaaggaaggg tttgctcact actttgaatt tgttggtaca gactacctct 1501 atcctggctg gaacatggaa aagcagaggt ttctgaccga tgttctgcat gaagtgatgc 1561 tgctggacgg tttggccagt tcccatccag tatcacagga agtgctgcag gcaacagata 1621 ttgacagggt gtttgactgg atcgcatata aaaagggtgc tgctttaata agaatgctgg 1681 ctaattttat gggccattca gttttccaga ggggtttgca agattattta accattcata 1741 agtatggtaa tgcagccaga aatgatctct ggaatacatt atcggaggct ttaaaaagaa 1801 atgggaaata tgtaaatata caagaagtaa tggatcagtg gacactccag atgggttatc 1861 ctgttatcac catcttggga aacacaacag cagaaaatag aataataatt acccaacagc 1921 attttatcta tgatatcagt gctaaaacta aagcacttaa acttcagaat aacagttacc 1981 tgtggcagat tccattaact attgtggtag gaaatagaag ccatgtgtct tcagaagcaa 2041 ttatttgggt gtctaacaaa tcagagcacc acagaataac ttatttggac aaaggaagct 2101 ggctgctggg gaacatcaat caaactggct attttagagt caactatgac ctaaggaact 2161 ggagattatt aattgatcaa ttaatccgga atcatgaggt tctttctgtc agtaaccgag 2221 cgggcttgat cgatgatgcc ttcagcctag ccagggctgg ctatttgcct cagaatattc 2281 ctctggagat tatcagatac ctgtctgagg agaaggattt tcttccttgg catgctgcca 2341 gccgagctct ttatcctcta gataaattac tggaccgcat ggaaaactac aacattttca 2401 atgaatatat tttaaagcaa gttgcaacaa catatatcaa gcttgggtgg ccgaaaaata 2461 attttaatgg atctcttgtt caagcatcct accaacatga agaactacgt agagaagtta 2521 taatgctggc ctgcagtttt ggcaacaagc actgtcacca acaggcatca acacttattt 2581 cagattggat ttccagcaac aggaacagaa taccactaaa tgttagagac atcgtatact 2641 gtacaggagt gtcactactg gatgaggatg tctgggaatt catatggatg aaattccatt 2701 ccaccacagc agtttctgag aagaaaatat tattggaagc cTTAACTTGC AGTGATGACA 2761 GGAATTTATT AAACAGGCTT CTAAATCTGT CACTGAATTC TGAGGTGGTG CTGGATCAAG 2821 ATGCAATTGA TGTCATAATC CATGTAGCTC GAAATCCACA TGGTCGAGAC CTTGCCTGGA 2881 AGTTTTTCAG GGATAAATGG AAGATATTAA ATACCAGGTA TGGAGAAGCA TTGTTTATGA 2941 ATTCCAAACT CATCAGTGGT GTCACAGAAT TTCTTAATAC TGAAGGTGAA CTCAAAGAGC 3001 TCAAGAACTT CATGAAAAAC TATGATGGGG TAGCTGCTGC TTCTTTCTCA CGAGCTGTGG 3061 AAACTGTCGA AGCCAATGTG CGCTGGAAAA TGCTTTACCA AGACGAGCTT TTCCAATGGT 3121 TAGGAAAAGC TCTAAGACAC TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 3181 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 3241 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGCTT AACCAACCCT 3301 CTTTTCAAAC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt