Construct: ORF TRCN0000477132
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014161.1_s317c1
- Derived from:
- ccsbBroadEn_04193
- DNA Barcode:
- GGGAGGCTATGTCTCGTGCAAGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAB11FIP1 (80223)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477132
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80223 | RAB11FIP1 | RAB11 family interacting pr... | NM_025151.5 | 100% | 100% | |
| 2 | human | 80223 | RAB11FIP1 | RAB11 family interacting pr... | XM_017013869.2 | 51.8% | 47.2% | (many diffs) |
| 3 | human | 80223 | RAB11FIP1 | RAB11 family interacting pr... | NM_001002814.3 | 50.5% | 50.5% | 1622_3523del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2016
- ORF length:
- 1947
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtccctaatg gtctcggctg gccggggcct gggggccgtg tggtccccaa 121 cccacgtgca ggtgacggtg ctgcaggcgc ggggcctgcg ggccaagggc cccgggggca 181 cgagcgacgc gtacgcggtg atccaggtgg gcaaggagaa gtacgccacc tccgtgtcgg 241 agcgcagcct gggcgcgccc gtgtggcgcg aggaggccac cttcgagctg ccatcgctgc 301 tgtcctccgg acccgcggcc gccgccaccc tgcagctcac cgtgctgcac cgcgcgctgc 361 tcggcctcga caagttcctg ggccgcgccg aggtggacct gcgggatctg caccgcgacc 421 agggccgcag gaagacgcag tggtataagt tgaaatccaa accaggaaag aaggacaagg 481 agcgaggaga aattgaggtt gacatccagt ttatgagaaa caacatgact gccagcatgt 541 ttgacctttc tatgaaagac aagtctcgga atccatttgg aaagctgaag gacaagatca 601 aggggaagaa taaggacagt gggtcagaca ccgcctccgc catcatccct agcacgacac 661 cttcggtcga cagtgatgat gagtctgtgg ttaaagacaa gaaaaagaaa tcaaagatca 721 agaccttact ttccaagtca aatttgcaga agacgcctct ttcccagtcc atgtctgtcc 781 tgccgacttc aaagccagaa aaagtgctgc ttcgtcccgg agactttcag tcccagtggg 841 atgaagatga caatgaggat gagtcctcct cggcctcgga tgtcatgtct cacaagagaa 901 cagcgagtac ggatcttaag caactgaacc aggtcaactt tacccttccc aagaaggaag 961 gactttcctt tcttggtggc cttcggtcta agaatgatgt cctttcccgc tctaatgtct 1021 gcatcaatgg gaaccatgtt tacctggagc agccagaagc caagggtgag atcaaggata 1081 gcagcccgtc ctcctcccca tcccccaagg gcttcagaaa gaagcatttg ttctcttcta 1141 cagagaacct ggcggctggg tcttggaagg agcctgctga aggaggtggg ctgtcttctg 1201 acaggcagct ctccgaatct tccaccaagg actccttgaa gtctatgacc ctgccgtcct 1261 accgacctgc cccactggtc agtggggacc tcagggaaaa catggccccc gcaaactcag 1321 aggccacaaa agaagctaag gagagcaaga agccagagag caggaggtcc tctttgctgt 1381 ctctgatgac ggggaagaag gatgtggcta agggcagtga aggtgaaaac cctctcacgg 1441 tcccagggag ggagaaggaa ggcatgctga tgggggttaa gccgggggag gacgcatcgg 1501 ggcctgctga agaccttgtg agaagatctg agaaagatac tgcagctgtt gtctccagac 1561 agggcagctc cctgaacctc tttgaagatg tgcagatcac agaaccagaa gcTGAGCCAG 1621 AGTCCAAGTC TGAACCGAGA CCTCCAATTT CCTCTCCGAG GGCTCCCCAG ACCAGAGCTG 1681 TCAAGCCCCG ACTTCATCCT GTGAAGCCAA TGAATGCAAT GGCCACCAAG GTTGCTAACT 1741 GCAGCTTGGG AACTGCCACC ATCATCAGTG AGAACTTGAA CAATGAGGTC ATGATGAAGA 1801 AATACAGCCC CTCGGACCCT GCATTTGCAT ATGCGCAGCT GACCCACGAT GAGCTGATTC 1861 AGCTGGTCCT CAAACAGAAG GAAACGATAA GCAAGAAGGA GTTCCAGGTC CGCGAGCTGG 1921 AAGACTACAT TGACAACCTG CTTGTCAGGG TCATGGAAGA AACCCCCAAT ATCCTCCGCA 1981 TCCCGACTCA GGTTGGCAAA AAAGCAGGAA AGATGTTGCC AACTTTCTTG TACAAAGTGG 2041 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2101 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2161 AGGGAGGCTA TGTCTCGTGC AAGGTACGCG TTAAGTCgac aatcaacctc tggattacaa 2221 aatttgtgaa agatt