Construct: ORF TRCN0000477135
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011441.1_s317c1
- Derived from:
- ccsbBroadEn_11850
- DNA Barcode:
- ATATCTGAGAGTGTTTGCAGCCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF777 (27153)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477135
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27153 | ZNF777 | zinc finger protein 777 | NM_015694.3 | 62.2% | 62.2% | 1_939del;1534G>A |
| 2 | human | 27153 | ZNF777 | zinc finger protein 777 | XM_011516055.2 | 62.2% | 62.2% | 1_939del;1534G>A |
| 3 | human | 27153 | ZNF777 | zinc finger protein 777 | XM_005249980.3 | 59.1% | 59% | 1_1071del;1666G>A |
| 4 | mouse | 72306 | Zfp777 | zinc finger protein 777 | XM_017321760.1 | 80.3% | 86.2% | (many diffs) |
| 5 | mouse | 72306 | Zfp777 | zinc finger protein 777 | NM_001347139.1 | 54.9% | 59.6% | (many diffs) |
| 6 | mouse | 72306 | Zfp777 | zinc finger protein 777 | XM_006506673.3 | 54.9% | 59.6% | (many diffs) |
| 7 | mouse | 72306 | Zfp777 | zinc finger protein 777 | XM_006506674.3 | 54.9% | 59.6% | (many diffs) |
| 8 | mouse | 72306 | Zfp777 | zinc finger protein 777 | XM_011241478.2 | 54.9% | 59.6% | (many diffs) |
| 9 | mouse | 72306 | Zfp777 | zinc finger protein 777 | XM_006506671.3 | 53.4% | 58% | (many diffs) |
| 10 | mouse | 72306 | Zfp777 | zinc finger protein 777 | NM_001081382.1 | 52.1% | 56.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1623
- ORF length:
- 1554
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaggggcaac tacgagtccc tggtttccat ggactatgca atttccaaac 121 cagacctcat gtcacagatg gagcgcgggg agcggcccac catgcaggag caggaagact 181 ctgaggaggg cgaaacgccg acagatccca gtgctgcgca cgatgggatc gtgattaaga 241 tcgaggtaca gaccaacgac gagggctcag aaagtttgga gacacctgag cccctgatgg 301 gacaggtgga agagcacggc ttccaggact cagagctggg tgacccctgt ggggaacagc 361 cagacctgga catgcaggag ccagagaaca cgctggagga gtccacggaa ggctccagcg 421 agttcagcga actgaagcag atgctggtgc agcagaggaa ctgcacggag gggatcgtga 481 tcaagacaga ggaacaagac gaggaggaag aagaggagga ggaggatgag ctgccgcagc 541 acttgcaatc ccttgggcag ctgtccggga gatatgaggc cagtatgtac cagaccccgc 601 tgcccgggga gatgtccccc gagggcgagg agagcccccc gcccctgcag ctaggaaacc 661 ccacagtgaa aaggctggcg ccctccgtgc acggtgagcg gcacctgagc gagaaccgcg 721 gggcctcgag ccagcagcag cggaaccggc gcggcgagcg gcccttcaca tgcatggagt 781 gcggcaagag cttccgcctg aagatcaacc tcatcatcca ccagcgcaac cacatcaagg 841 aggggcccta cgagtgcgcc gaatgcgaga tcagcttccg gcacaagcaa cagctcacgc 901 tgcaccagcg catccaccgc gtgcgcggag gctgcgtctc acccgaacgc gggcccacgt 961 tcaaccccaa gcacgcgctc aagccgcgtc ccaagtcacc cagctctggt agcggcggcg 1021 gtggccctaa gccctacaag tgccccgagt gcgacagcag cttcagccac aagtccagcc 1081 tgaccaaaca ccagatcacg cacacgggtg agcggcccta cacgtgcccc gagtgcaaga 1141 agagcttccg cctgcacatc agcttggtga tccatcagcg cgtgcacgcg ggcaagcatg 1201 aggtctcctt catctgcagc ctgtgcggca agagcttcag ccgcccctcg cacctgctgc 1261 gccaccagcg gactcacaca ggcgagcggc ccTTCAAGTG CCCCGAGTGC GAGAAGAGCT 1321 TCAGCGAGAA GTCCAAGCTC ACCAACCACT GCCGCGTGCA CTCGCGCGAG CGGCCGCACG 1381 CCTGCCCCGA GTGCGGCAAG AGCTTCATCC GCAAGCACCA CCTCCTGGAA CACCGGCGCA 1441 TCCACACAGG CGAGCGGCCC TACCACTGCG CCGAGTGCGG CAAGCGCTTC ACGCAGAAGC 1501 ATCACCTGCT GGAGCACCAG CGCGCGCACA CGGGCGAGCG GCCCTACCCC TGCACGCACT 1561 GCGCCAAGTG CTTCCGCTAC AAGCAGTCGC TCAAGTACCA CCTGCGGACC CACACGGGCG 1621 AGTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1681 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1741 GGCTTTATAT ATCTTGTGGA AAGGACGAAT ATCTGAGAGT GTTTGCAGCC TAACGCGTTA 1801 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt