Construct: ORF TRCN0000477137
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009952.1_s317c1
- Derived from:
- ccsbBroadEn_01798
- DNA Barcode:
- TACATTTTATAAATGTTAGCGCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF35 (7584)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477137
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7584 | ZNF35 | zinc finger protein 35 | NM_003420.4 | 100% | 100% | |
| 2 | mouse | 22646 | Zfp105 | zinc finger protein 105 | NM_009544.2 | 83.9% | 81% | (many diffs) |
| 3 | mouse | 22646 | Zfp105 | zinc finger protein 105 | XM_011242959.2 | 83.9% | 81% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1650
- ORF length:
- 1581
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gactgcagaa ttgagagaag ccatggccct agccccatgg ggcccagtga 121 aggtgaaaaa ggaggaggaa gaagaagaaa acttcccagg tcaggcatcc agccaacaag 181 tgcactccga gaacatcaaa gtctgggccc cagtgcaggg tcttcagaca ggccttgatg 241 gatcagaaga ggaagaaaag ggtcagaaca tatcctggga tatggcggta gtcctgaaag 301 caactcagga ggcacctgct gcttcaaccc ttggcagcta ctcattacca gggactctgg 361 ccaagagtga gatactggag actcatggga ccatgaactt tctaggtgct gaaaccaaga 421 acctacagtt actggttcca aaaactgaga tatgtgagga agctgaaaaa cccctcatca 481 tatcagaaag aatccagaaa gctgatcctc aaggacctga gttaggagaa gcttgtgaaa 541 agggaaacat gttaaagagg cagagaataa agagagaaaa gaaagatttc agacaagtga 601 tagtgaatga ctgtcactta cctgaaagct tcaaagaaga ggaaaaccag aaatgtaaga 661 aatctggagg aaaatatagc cttaattctg gcgctgttaa aaatccaaaa acccagcttg 721 gacaaaagcc ttttacgtgt agcgtgtgtg ggaaaggatt tagtcagagt gcaaacctcg 781 ttgtgcatca gcgaatccac actggagaga aaccctttga atgtcatgag tgtgggaagg 841 ccttcattca gagtgcaaac ctcgttgtgc atcagagaat ccacactgga cagaaacctt 901 atgtttgctc aaaatgtggg aaagccttca ctcagagttc aaatctgact gtacatcaaa 961 aaatccactc cttagaaaaa acttttaagt gcaatgaatg tgagaaagcc tttagttaca 1021 gctcacaact tgctcggcac cagaaagtcc acattacgga aaaatgctat gaatgtaatg 1081 aatgtgggaa aacatttact aggagctcaa acctcattgt ccaccagagg atccacactg 1141 gggagaagcc ctttgcctgt aacgactgtg gcaaagcctt tacccagagt gcaaatctta 1201 ttgtacatca gcgaagccat actggtgaga agccatatga gtgtaaagag tgtgggaaag 1261 cctttagttg tttttcacac cttattgtgc accagagaat tcacactgca gagaaacctt 1321 acgactgcag cgaatgtggg aaagccttca gtcagctctc ttgccTTATT GTCCACCAGA 1381 GAATTCACAG TGGAGATCTT CCTTACGTGT GTAATGAATG TGGGAAGGCC TTCACATGTA 1441 GCTCATACCT ACTTATTCAT CAGAGAATTC ATAATGGAGA AAAACCTTAC ACATGTAATG 1501 AGTGTGGGAA GGCCTTCAGA CAGAGGTCGA GCCTCACCGT GCACCAGAGA ACCCACACTG 1561 GGGAGAAGCC CTATGAATGT GAGAAGTGTG GTGCAGCTTT CATTTCCAAC TCACACCTCA 1621 TGCGACACCA TAGAACCCAT CTTGTTGAAT TGCCAACTTT CTTGTACAAA GTGGTTGATA 1681 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1741 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATACAT 1801 TTTATAAATG TTAGCGCCCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1861 tgaaagatt