Construct: ORF TRCN0000477138
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009008.1_s317c1
- Derived from:
- ccsbBroadEn_07708
- DNA Barcode:
- GTCGTGATGTGGTTCGTTCTCGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EHD1 (10938)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477138
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10938 | EHD1 | EH domain containing 1 | NM_001282444.2 | 99.8% | 100% | 66T>C;1005C>G |
| 2 | human | 10938 | EHD1 | EH domain containing 1 | NM_006795.4 | 99.8% | 100% | 66T>C;1005C>G |
| 3 | human | 10938 | EHD1 | EH domain containing 1 | NM_001282445.1 | 97.4% | 97.4% | 1_42del |
| 4 | human | 30845 | EHD3 | EH domain containing 3 | NM_014600.3 | 82.1% | 86.5% | (many diffs) |
| 5 | mouse | 13660 | Ehd1 | EH-domain containing 1 | NM_010119.5 | 91% | 99.4% | (many diffs) |
| 6 | mouse | 57440 | Ehd3 | EH-domain containing 3 | NM_020578.3 | 79.3% | 86.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1671
- ORF length:
- 1602
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gttcagctgg gtcagcaagg atgcccgccg caagaaggag ccggagctct 121 tccagacggt ggccgagggg ctgcggcagc tgtacgcgca gaagctgcta cccctggagg 181 agcactaccg cttccacgag ttccactcgc ccgcgctgga ggacgctgac ttcgacaaca 241 agcctatggt gctcctcgtg gggcagtaca gcacgggcaa gaccaccttc atccgacacc 301 tgatcgagca ggacttcccg gggatgcgca tcgggcccga gcccaccacc gactccttca 361 tcgccgtcat gcacggcccc actgagggcg tggtgccggg caacgcgctc gtggtggacc 421 cgcggcgccc cttccgcaag ctcaacgcgt ttggcaacgc tttcctcaac aggttcatgt 481 gtgcccagct gcccaacccc gtcctggaca gcatcagcat catcgacacc cccgggatcc 541 tgtctggaga gaagcagcgg atcagcagag gctatgactt tgcagccgtc ctggagtggt 601 tcgcggagcg tgtggaccgc atcatcctgc tcttcgacgc ccacaagctg gacatctccg 661 atgagttctc ggaagtgatc aaggctctga agaaccatga ggacaagatc cgcgtggtgc 721 tgaacaaggc agaccagatc gagacgcagc agctgatgcg ggtgtacggg gccctcatgt 781 ggtccctggg caagatcatc aacacccccg aggtggtcag ggtctacatc ggctccttct 841 ggtcccaccc gctcctcatc cccgacaacc gcaagctctt tgaggccgag gagcaggacc 901 tcttcaagga catccagtca ctgccccgaa acgccgccct caggaagctc aatgacctga 961 tcaagcgggc acggctggcc aaggttcacg cctacatcat cagctccctc aagaaagaga 1021 tgcccaatgt ctttggtaaa gagagcaaaa agaaagagct ggtgaacaac ctgggagaga 1081 tctaccagaa gattgagcgc gagcaccaga tctcccctgg ggacttcccg agcctccgca 1141 agatgcagga actcctgcag acccaggact tcagcaagtt ccaggcgctg aagcccaagc 1201 tgctggacac ggtggatgac atgctggcca acgacatcgc gcggctgatg gtgatggtgc 1261 ggcaggagga gtccctgatg ccttcccagg tggtcaaggg cggcgccttt gacggcacca 1321 tgaacgggcc gttcgggcac ggctacggcg agggggccgg cgagggcatc gacgacgtgg 1381 agtgggtggt gggcaaggac aagcccaccT ACGACGAGAT CTTCTACACG CTGTCCCCTG 1441 TCAACGGCAA GATCACGGGC GCCAACGCCA AGAAGGAGAT GGTGAAGTCC AAGCTCCCCA 1501 ACACCGTGCT AGGGAAGATC TGGAAGCTGG CCGACGTGGA CAAGGACGGG CTGCTGGACG 1561 ACGAGGAGTT CGCGCTGGCC AACCACCTCA TCAAGGTCAA GCTGGAGGGC CACGAGCTGC 1621 CCGCCGACCT GCCCCCGCAC CTGGTGCCGC CCTCCAAGCG CAGACATGAG TTGCCAACTT 1681 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1741 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1801 CTTGTGGAAA GGACGAGTCG TGATGTGGTT CGTTCTCGGT ACGCGTTAAG TCgacaatca 1861 acctctggat tacaaaattt gtgaaagatt