Construct: ORF TRCN0000477158

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010329.1_s317c1
Derived from:
ccsbBroadEn_13965
DNA Barcode:
CCCGCTCATGGGATCTCCAACAGT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
ZEB1 (6935)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477158
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6935 ZEB1 zinc finger E-box binding h... NM_001174096.1 99.9% 68.6% 2321_2322insA
2 human 6935 ZEB1 zinc finger E-box binding h... NM_030751.6 99.8% 68.4% 259_260insCAG;2318_2319insA
3 human 6935 ZEB1 zinc finger E-box binding h... NM_001323676.2 98.5% 66.9% (many diffs)
4 human 6935 ZEB1 zinc finger E-box binding h... NM_001323677.2 98.4% 66.7% (many diffs)
5 human 6935 ZEB1 zinc finger E-box binding h... NM_001128128.2 98.3% 66.9% (many diffs)
6 human 6935 ZEB1 zinc finger E-box binding h... NM_001174094.2 98.2% 66.7% (many diffs)
7 human 6935 ZEB1 zinc finger E-box binding h... NM_001174093.2 98.1% 66.6% 259_260ins63;2258_2259insA
8 human 6935 ZEB1 zinc finger E-box binding h... NM_001174095.2 93.9% 62.6% 57_58ins204;2117_2118insA
9 human 6935 ZEB1 zinc finger E-box binding h... NM_001323674.2 93.3% 61.9% 259_260ins225;2096_2097insA
10 human 6935 ZEB1 zinc finger E-box binding h... NM_001323675.2 91.9% 60.2% (many diffs)
11 human 6935 ZEB1 zinc finger E-box binding h... NM_001323678.2 91.7% 60.2% (many diffs)
12 human 6935 ZEB1 zinc finger E-box binding h... NM_001323638.2 80.5% 49.4% 0_1ins657;1664_1665insA
13 human 6935 ZEB1 zinc finger E-box binding h... NM_001323641.2 80.5% 49.4% 0_1ins657;1664_1665insA
14 human 6935 ZEB1 zinc finger E-box binding h... NM_001323642.2 80.5% 49.4% 0_1ins657;1664_1665insA
15 human 6935 ZEB1 zinc finger E-box binding h... NM_001323643.2 80.5% 49.4% 0_1ins657;1664_1665insA
16 human 6935 ZEB1 zinc finger E-box binding h... NM_001323644.2 80.5% 49.4% 0_1ins657;1664_1665insA
17 human 6935 ZEB1 zinc finger E-box binding h... NM_001323645.2 80.5% 49.4% 0_1ins657;1664_1665insA
18 human 6935 ZEB1 zinc finger E-box binding h... NM_001323646.2 80.5% 49.4% 0_1ins657;1664_1665insA
19 human 6935 ZEB1 zinc finger E-box binding h... NM_001323647.2 80.5% 49.4% 0_1ins657;1664_1665insA
20 human 6935 ZEB1 zinc finger E-box binding h... NM_001323648.2 80.5% 49.4% 0_1ins657;1664_1665insA
21 human 6935 ZEB1 zinc finger E-box binding h... NM_001323649.2 80.5% 49.4% 0_1ins657;1664_1665insA
22 human 6935 ZEB1 zinc finger E-box binding h... NM_001323650.2 80.5% 49.4% 0_1ins657;1664_1665insA
23 human 6935 ZEB1 zinc finger E-box binding h... NM_001323651.2 80.5% 49.4% 0_1ins657;1664_1665insA
24 human 6935 ZEB1 zinc finger E-box binding h... NM_001323652.2 80.5% 49.4% 0_1ins657;1664_1665insA
25 human 6935 ZEB1 zinc finger E-box binding h... NM_001323653.2 80.5% 49.4% 0_1ins657;1664_1665insA
26 human 6935 ZEB1 zinc finger E-box binding h... NM_001323654.2 80.5% 49.4% 0_1ins657;1664_1665insA
27 human 6935 ZEB1 zinc finger E-box binding h... NM_001323655.2 80.5% 49.4% 0_1ins657;1664_1665insA
28 human 6935 ZEB1 zinc finger E-box binding h... NM_001323656.2 80.5% 49.4% 0_1ins657;1664_1665insA
29 human 6935 ZEB1 zinc finger E-box binding h... NM_001323657.2 80.5% 49.4% 0_1ins657;1664_1665insA
30 human 6935 ZEB1 zinc finger E-box binding h... NM_001323658.2 80.5% 49.4% 0_1ins657;1664_1665insA
31 human 6935 ZEB1 zinc finger E-box binding h... NM_001323659.2 80.5% 49.4% 0_1ins657;1664_1665insA
32 human 6935 ZEB1 zinc finger E-box binding h... NM_001323660.2 80.5% 49.4% 0_1ins657;1664_1665insA
33 human 6935 ZEB1 zinc finger E-box binding h... NM_001323661.2 80.5% 49.4% 0_1ins657;1664_1665insA
34 human 6935 ZEB1 zinc finger E-box binding h... NM_001323662.2 80.5% 49.4% 0_1ins657;1664_1665insA
35 human 6935 ZEB1 zinc finger E-box binding h... NM_001323663.2 80.5% 49.4% 0_1ins657;1664_1665insA
36 human 6935 ZEB1 zinc finger E-box binding h... NM_001323664.2 80.5% 49.4% 0_1ins657;1664_1665insA
37 human 6935 ZEB1 zinc finger E-box binding h... NM_001323665.2 80.5% 49.4% 0_1ins657;1664_1665insA
38 human 6935 ZEB1 zinc finger E-box binding h... NM_001323666.2 80.5% 49.4% 0_1ins657;1664_1665insA
39 human 6935 ZEB1 zinc finger E-box binding h... NM_001323671.2 80.5% 49.4% 0_1ins657;1664_1665insA
40 human 6935 ZEB1 zinc finger E-box binding h... NM_001323672.2 80.5% 49.4% 0_1ins657;1664_1665insA
41 human 6935 ZEB1 zinc finger E-box binding h... NM_001323673.2 80.5% 49.4% 0_1ins657;1664_1665insA
42 human 6935 ZEB1 zinc finger E-box binding h... XM_006717498.2 80.5% 49.4% 0_1ins657;1664_1665insA
43 human 6935 ZEB1 zinc finger E-box binding h... XM_011519643.2 80.5% 49.4% 0_1ins657;1664_1665insA
44 human 6935 ZEB1 zinc finger E-box binding h... XM_017016597.1 80.5% 49.4% 0_1ins657;1664_1665insA
45 mouse 21417 Zeb1 zinc finger E-box binding h... NM_011546.3 83.2% 57.9% (many diffs)
46 mouse 21417 Zeb1 zinc finger E-box binding h... XM_006525769.2 82.1% 56.3% (many diffs)
47 mouse 21417 Zeb1 zinc finger E-box binding h... XM_006525772.3 81.6% 56.2% (many diffs)
48 mouse 21417 Zeb1 zinc finger E-box binding h... XM_006525775.3 78.6% 53.3% (many diffs)
49 mouse 21417 Zeb1 zinc finger E-box binding h... XM_017317864.1 78.6% 53.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2526
ORF length:
2457
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggcggatggc cccaggtgta agcgcagaaa gcaggcgaac ccgcggcgca
121ataacgttac aaattataat actgtggtag aaacaaattc agattcagat gatgaagaca
181aactgcatat tgtggaagaa gaaagtgtta cagatgcagc tgactgtgaa ggtgtaccag
241aggatgacct gccaacagac cagacagtgt taccagggag gagcagtgaa agagaaggga
301atgctaagaa ctgctgggag gatgacacag gaaaggaagg gcaagaaatc ctggggcctg
361aagctcaggc agatgaagca ggatgtacag taaaagatga tgaatgcgag tcagatgcag
421aaaatgagca aaaccatgat cctaatgttg aagagtttct acaacaacaa gacactgctg
481tcatttttcc tgaggcacct gaagaggacc agaggcaggg cacaccagaa gccagtggtc
541atgatgaaaa tggaacacca gatgcatttt cacaattact cacctgtcca tattgtgata
601gaggctataa acgctttacc tctctgaaag aacacattaa atatcgtcat gaaaagaatg
661aagataactt tagttgctcc ctgtgcagtt acacctttgc atacagaacc caacttgaac
721gtcacatgac atcacataaa tcaggaagag atcaaagaca tgtgacgcag tctgggtgta
781atcgtaaatt caaatgcact gagtgtggaa aagctttcaa atacaaacat cacctaaaag
841agcacttaag aattcacagt ggagagaagc catatgaatg cccaaactgc aagaaacgct
901tttcccattc tggctcctat agctcacaca taagcagtaa gaaatgtatc agcttgatac
961ctgtgaatgg gcgaccaaga acaggactca agacatctca gtgttcttca ccgtctcttt
1021cagcatcacc aggcagtccc acacgaccac agatacggca aaagatagag aataaacccc
1081ttcaagaaca actttctgtt aaccaaatta aaactgaacc tgtggattat gaattcaaac
1141ccatagtggt tgcttcagga atcaactgtt caaccccttt acaaaatggg gttttcactg
1201gtggtggccc attacaggca accagttctc ctcagggcat ggtgcaagct gttgttctgc
1261caacagttgg tttggtgtct cccataagta tcaatttaag tgatattcag aatgtactta
1321aagtggcggt agatggtaat gtaataaggc aagtgttgga gaataatcaa gccaatcttg
1381catccaaaga acaagaaaca atcaatgctt cacccataca acaaggtggc cattctgtta
1441tttcagccat cagtcttcct ttggttgatc aagatggaac aaccaaaatt atcatcaact
1501acagtcttga gcagcctagc caacttcaag ttgttcctca aaatttaaaa aaagaaaatc
1561cagtcgctac aaacagttgt aaaagtgaaa agttaccaga agatcttact gttaagtctg
1621agaaggacaa aagctttgaa gggggggtga atgatagcac ttgtcttctg tgtgatgatt
1681gtccaggaga tattaatgca cttccagaat taaagcacta tgacctaaag cagcctactc
1741agcctcctcc actccctgca gcagaagctg agaagcctga gtcctctgtt tcatcagcta
1801ctggagatgg caatttgtct cctagtcagc cacctttaaa gaacctcttg tctctcctaa
1861aagcatatta tgctttgaat gcacaaccaa gtgcagaaga gctctcaaaa attgctgatt
1921cagtaaacct accactggat gtagtaaaaa agtggtttga aaagatgcaa gctggacaga
1981tttcagtgca gtcttctgaa ccatcttctc ctgaaccagg caaagtaaat atccctgcca
2041agaacaatga tcagcctcaa tctgcaaatg caaatgaacc ccaggacagc acagtaaatc
2101tacaaagtcc tttgaagatg actaactccc cagttttacc agtgggatca accaccaatg
2161gttccagaag tagtacacca tccccatcac ctctaaacct ttcctcatcc agaaatacac
2221agggttactt gtacacagct gagggtgcac aagaagagcc acaagtagaa cctcttgatc
2281tttcactacc aaagcaacag ggagaattat tagaaaggtc aactatcact agtgtttacc
2341agaacagtgt ttattctgtc caggaagaac ccttgaactt gtcttgcgca aaaaaaggag
2401ccacaaaagg acagttgtgt tacagactca gaaccagttg taaatgtaat cccaccaagt
2461gccaacccca taaatatcgc tatacctaca gtcactgccc agttacccac aatcgtggcc
2521attgctgacc agaacagtgt tccatgctta agagcgctag ctgccaataa gcaaacgatt
2581ctgattcccc aggtggcata cacctactca actacggtca gccctgcagt ccaagaacca
2641cccttgaaag tgatccagcc aaatggaaat caggatgaaa gacaagatac tagctcagaa
2701ggagtatcaa atgtagagga tcagaatgac tctgattcta caccgcccaa aaagaaaatg
2761cggaagacag aaaatggaat gtatgcttgt gatttgtgtg acaagatatt ccaaaagagt
2821agttcattat tgagacataa atatgaacac acaggtaaaa gacctcatga gtgtggaatc
2881tgtaaaaagg catttaaaca caaacatcat ttgattgaac acatgcgatt acattctgga
2941gaaaagccct atcaatgtga caaatgtgga aagcgcttct cacactctgg gtcttattct
3001caacacatga atcatcgcta ctcctactgt aagagagaag cggaagaacg tgacagcaca
3061gagcaggaag aggcagggcc tgaaatcctc tcGAATGAGC ACGTGGGTGC CAGGGCGTCT
3121CCCTCACAGG GCGACTCGGA CGAGAGAGAG AGTTTGACAA GGGAAGAGGA TGAAGACAGT
3181GAAAAAGAGG AAGAGGAGGA GGATAAAGAG ATGGAAGAAT TGCAGGAAGA AAAAGAATGT
3241GAAAAACCAC AAGGGGATGA GGAAGAGGAG GAGGAGGAGG AAGAAGTGGA AGAAGAAGAG
3301GTAGAAGAGG CAGAGAATGA GGGAGAAGAA GCAAAAACTG AAGGTCTGAT GAAGGATGAC
3361AGGGCTGAAA GTCAAGCAAG CAGCTTAGGA CAAAAAGTAG GCGAGAGTAG TGAGCAAGTG
3421TCTGAAGAAA AGACAAATGA AGCCTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT
3481AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA
3541CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CCCGCTCATG
3601GGATCTCCAA CAGTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa
3661gatt

Download FASTA (ORF) (Full)