Construct: ORF TRCN0000477159
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006908.1_s317c1
- Derived from:
- ccsbBroadEn_09202
- DNA Barcode:
- AACCAGCCAACTTCCTATCCCACA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC12A8 (84561)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477159
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84561 | SLC12A8 | solute carrier family 12 me... | NM_001195483.1 | 99.9% | 99.8% | 1991G>A;1998T>C |
| 2 | human | 84561 | SLC12A8 | solute carrier family 12 me... | NM_024628.5 | 99.9% | 99.8% | 1991G>A;1998T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2211
- ORF length:
- 2142
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gacccagatg tcccaggtgc aggagctctt ccatgaggca gcccagcagg 121 atgccctggc ccagccccag ccctggtgga agacccagct gttcatgtgg gagcctgtgc 181 tgtttgggac ctgggatggt gtgttcacat cctgcatgat caacatcttt ggggttgtgc 241 tcttcctgag gactggctgg ctggtgggaa acacaggagt gctcctgggc atgttcctgg 301 tgtccttcgt catcctggtg gccctcgtca cggtgctgtc tggcattggc gtcggggagc 361 gcagcagcat cggcagcggt ggcgtctact ccatgatctc ctcggtcctg ggtgggcaga 421 cgggaggcac catcgggctg ctctatgtgt ttggacagtg tgttgcaggt gccatgtata 481 tcaccggctt tgctgaatcc atctcggatt tgctgggcct cgggaatatc tgggctgtgc 541 gaggaatttc agttgcggtg cttctggcct tgctgggcat taacctcgca ggtgtcaaat 601 ggataatccg cctccagctg ctgttgctgt tcctgctggc cgtgtccaca ctggactttg 661 tggtgggttc tttcacccac ctggacccag aacatggttt cattggatat tcacccgaac 721 tgctacagaa caacacgctg cccgattaca gcccggggga atcttttttc actgtctttg 781 gggttttctt cccagcggct acaggagtca tggccggctt caacatgggg ggcgacctca 841 gggagcctgc cgccagcatt cccctgggct ccctggcagc tgttggcatc tcgtggtttc 901 tgtacatcgt cttcgtcttc ctcctgggcg ccatctgcac tcgagaggcc cttcgctatg 961 acttcctgat agcggaaaag gtatccctca tgggcttcct gttccttttg ggcttataca 1021 tctcgtccct ggcttcctgc atgggaggac tttatggagc tccccgcatc ctgcagtgca 1081 ttgcccagga gaaagtgatc cctgcacttg cctgtctggg acaagggaag gggccaaaca 1141 aaacacccgt ggctgccatc tgcctgacca gcttggtgac catggccttt gtttttgtgg 1201 gtcaagtgaa cgttctggcc cccatcgtca ccatcaactt catgctgaca tacgttgcag 1261 tggactactc ttacttctcc ctgtccatgt gttcctgcag cctgaccccg gtgcctgagc 1321 cggtgctcag ggagggcgca gaaggcctcc actgctctga gcacctgctc ttagagaaag 1381 ctcccagtta cggctctgag ggacctgccc aaagagtctt ggagggcacg ctactggaat 1441 tcaccaagga catggatcag ctcctccagc taaccaggaa gcttgagagt agccagccca 1501 ggcaaggaga gggtaacagg accccagaaa gtcagaagag gaaaagcaag aaggccacca 1561 agcagaccct acaagatagc ttcctcttgg acctcaaatc ccctccttct ttccctgtcg 1621 agatctctga caggttgccc gctgcctcct gggaggggca ggagtcctgc tggaacaagc 1681 agacttccaa gagcgaaggg actcagcctg agggaacata tggagagcaa cttgttcctg 1741 agctgtgcaa ccaatcagag tccagtggag aagatttctt ccTGAAGTCC AGGCTCCAAG 1801 AACAAGATGT CTGGAGAAGA TCCACTTCTT TCTATACCCA CATGTGCAAC CCCTGGGTCT 1861 CCCTGTTGGG GGCTGTTGGG TCCCTTCTCA TCATGTTTGT GATACAGTGG GTGTATACCC 1921 TGGTTAACAT GGGTGTTGCT GCCATCGTGT ATTTCTACAT TGGCCGGGCC AGTCCAGGGC 1981 TTCACCTTGG ATCAGCCTCC AACTTCAGCT TTTTCCGGTG GATGAGGTCT CTCTTGCTCC 2041 CCTCCTGCAG GAGCTTGCAG TCCCCCCAGG AGCAGATCAT CTTGGCGCCG TCCCTGGCTA 2101 AGGTTGACAT GGAGATGACT CAGCTCACCC AGGAGAATGC AGACTTCGCC ACTCGGGATC 2161 GCTACCACCA CTCCTCCCTC GTGAACCGGG AGCAGCTGAT GCCTCACTAC TTGGCCAACT 2221 TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT 2281 TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA 2341 TCTTGTGGAA AGGACGAAAC CAGCCAACTT CCTATCCCAC AACGCGTTAA GTCgacaatc 2401 aacctctgga ttacaaaatt tgtgaaagat t