Construct: ORF TRCN0000477171
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004450.1_s317c1
- Derived from:
- ccsbBroadEn_14048
- DNA Barcode:
- GCAGCCTCTACCCCGGGCTTGGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEMA4D (10507)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477171
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10507 | SEMA4D | semaphorin 4D | NM_001142287.2 | 16.1% | 14.8% | (many diffs) |
| 2 | human | 10507 | SEMA4D | semaphorin 4D | NM_001371198.1 | 16.1% | 14.8% | (many diffs) |
| 3 | human | 10507 | SEMA4D | semaphorin 4D | NM_001371199.1 | 16.1% | 14.8% | (many diffs) |
| 4 | human | 10507 | SEMA4D | semaphorin 4D | NM_001371200.1 | 16.1% | 14.8% | (many diffs) |
| 5 | human | 10507 | SEMA4D | semaphorin 4D | NM_001371201.1 | 16.1% | 14.8% | (many diffs) |
| 6 | human | 10507 | SEMA4D | semaphorin 4D | XR_001746156.1 | 11.3% | (many diffs) | |
| 7 | human | 10507 | SEMA4D | semaphorin 4D | XR_001746153.1 | 11.2% | (many diffs) | |
| 8 | human | 10507 | SEMA4D | semaphorin 4D | NR_163886.1 | 8.9% | (many diffs) | |
| 9 | human | 10507 | SEMA4D | semaphorin 4D | NR_163887.1 | 8.8% | (many diffs) | |
| 10 | human | 10507 | SEMA4D | semaphorin 4D | NR_163884.1 | 8.8% | (many diffs) | |
| 11 | human | 10507 | SEMA4D | semaphorin 4D | NR_163885.1 | 8.7% | (many diffs) | |
| 12 | human | 10507 | SEMA4D | semaphorin 4D | NR_163888.1 | 8.6% | (many diffs) | |
| 13 | human | 10507 | SEMA4D | semaphorin 4D | NR_163889.1 | 8.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 438
- ORF length:
- 369
- Sequence:
-
1 tcTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCACCAT GTGCACTCAG AGGTGGAAAC GGTCTGTGTC CCCAGGTCCT GCAGACATTC 121 GCTTTGTCTG GGAGAAGAAT GGGCGAGCTC TGGAGACCTG TGTCCCTGTG CAGACCCATG 181 CACTGCCCGA TGGCAGGGCC CATGCACTCA GCTGGCTGCA GGACGCCATC AGGGAAAGCG 241 CTGAGTATCG CTGCTCTGTC CTCTACTCAG CAGGGAACAA GACTTCGAAG GTGCAGGTTG 301 CTGTGATGAG ACCTGAAGTG ACCCACCAGG AGAGGTGGAC CAGAGAGCTC TCTGCCTGGA 361 GGGCTGTGGC TGGGGAGCAC GACCGGATGA TGCAGAGCTG GAGGAAGGCG TGGGAAAGCT 421 GTAGCAAGGA CACCCTGTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 481 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 541 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGCAGCCT CTACCCCGGG 601 CTTGGTTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt