Construct: ORF TRCN0000477196
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012676.1_s317c1
- Derived from:
- ccsbBroadEn_14003
- DNA Barcode:
- TTAATAACCAAAATTCCCTTCCCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- URI1 (8725)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477196
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8725 | URI1 | URI1 prefoldin like chaperone | XM_011527435.2 | 97.8% | 34.3% | (many diffs) |
| 2 | human | 8725 | URI1 | URI1 prefoldin like chaperone | XM_005259362.2 | 94.6% | 33.2% | (many diffs) |
| 3 | human | 8725 | URI1 | URI1 prefoldin like chaperone | XM_024451751.1 | 92.4% | 29.2% | 0_1ins108;436G>T;666_668delTGA |
| 4 | human | 8725 | URI1 | URI1 prefoldin like chaperone | NM_003796.3 | 92.2% | 32.1% | (many diffs) |
| 5 | human | 8725 | URI1 | URI1 prefoldin like chaperone | NM_001252641.2 | 85% | 36.2% | (many diffs) |
| 6 | human | 8725 | URI1 | URI1 prefoldin like chaperone | XM_005259363.4 | 82.9% | 34.8% | (many diffs) |
| 7 | human | 8725 | URI1 | URI1 prefoldin like chaperone | NR_045557.1 | 43.2% | (many diffs) | |
| 8 | mouse | 19777 | Uri1 | URI1, prefoldin-like chaperone | XM_006539678.3 | 74.1% | 25.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 612
- ORF length:
- 543
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtggcgcggc tgcgcgagga gcaggaaaag gaagaaggta gataatgact 121 ataatgccct tcgagaaaga ctcagcacct tgcctgataa attgtcttat aatataatgg 181 taccatttgg cccttttgcc ttcatgccag gaaaacttgt ccatactaat gaagtcactg 241 ttttactggg ggacaactgg tttgcaaagt gctcagcaaa gcaggctgta ggtttagttg 301 agcaccggaa agaacatgta agaaaaacaa tagatgactt aaaaaaagtg atgaaaaatt 361 ttgaatccag agttgaattc acagaagatt tgcagaaaat gagcgatgct gcaggtgata 421 ttgttgacat acgagaagaa attaaatgtg acttcgaatt taaagcaaaa caccgaattg 481 ctcataaacc gcattccaaa ccaaaaactt cagatatttt tgaagcagat attgcaaatg 541 atgtgaaatc caaggatttg ctagctgata aagaactgtg ggctcgactt gaagaactag 601 agagacagga ataattgctg ggtgaacttg atagtaagcc tgatactgtg attgcaaatg 661 gagaagatac gacatcttct gaagaggaaa aggaagatcg taacacaaat gtgaatgcga 721 tgcatcaagt aacagactct catactcctt gtcataagga tgttgcaagt tcagaaccat 781 tcagtggtca agtgaatagt cagttgaact gttcagtgaa tggttccagt tcttaccaca 841 gtgatgatga tgatgatgat gatgacgacg acgacgacaa cattgacgac gatgatggtg 901 ataacgacca tgaggcttta ggggttggag ataattctat accaacaata tatttttcac 961 atactgttga gcctaagagg gtccgaataa atactggaaa gaataccact ttaaaattca 1021 gtgaaaagaa agaagaagcc aaacgtaaac gaaagaacag cactggcagt ggccactctg 1081 cccaggagct gccgaccatc aggacgcctg cagacattta cagagccttt gttgatgttg 1141 tgaatggaga atatgtccct cgcaaatcca TCCTGAAGTC TCGAAGTAGA GAGAATAGTG 1201 TGTGTAGCGA CACTAGTGAA AGCAGTGCTG CTGAATTTGA TGATAGGCGG GGAGTTTTGA 1261 GGAGTATCAG CTGCGAAGAA GCCACTTGCA GTGACACCAG TGAGAGCATT TTGGAAGAGG 1321 AACCACAAGA AAATCAAAAG AAACTTTTGC CCTTATCAGT AACACCTGAG GCTTTTTCTG 1381 GAACTGTTAT AGAAAAAGAA TTTGTATCAC CTTCCTTAAC ACCACCCCCA GCCATTGCTC 1441 ATCCCGCACT ACCCACTATT CCAGAACGAA AGGAAGTTCT GTTGGAAGCA TCTGAAGAAA 1501 CTGGAAAGAG GGTTTCAAAG TTTAAAGCTG CCAGATTGCA ACAGAAAGAC TTGCCAACTT 1561 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1621 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1681 CTTGTGGAAA GGACGATTAA TAACCAAAAT TCCCTTCCCA ACGCGTTAAG TCgacaatca 1741 acctctggat tacaaaattt gtgaaagatt