Construct: ORF TRCN0000477200
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018452.1_s317c1
- Derived from:
- ccsbBroadEn_00949
- DNA Barcode:
- TTGATACTAAGTGCATACAAACCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LRPAP1 (4043)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477200
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4043 | LRPAP1 | LDL receptor related protei... | NM_002337.4 | 100% | 100% | |
| 2 | human | 4043 | LRPAP1 | LDL receptor related protei... | XR_002959730.1 | 17.6% | 1_85del;836_2670del;2992_6080del | |
| 3 | human | 4043 | LRPAP1 | LDL receptor related protei... | NR_110005.1 | 9.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1140
- ORF length:
- 1071
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgccgcgg agggtcaggt cgtttctgcg cgggctcccg gcgctgctac 121 tgctgctgct cttcctcggg ccctggcccg ctgcgagcca cggcggcaag tactcgcggg 181 agaagaacca gcccaagccg tccccgaaac gcgagtccgg agaggagttc cgcatggaga 241 agttgaacca gctgtgggag aaggcccagc gactgcatct tcctcccgtg aggctggccg 301 agctccacgc tgatctgaag atacaggaga gggacgaact cgcctggaag aaactaaagc 361 ttgacggctt ggacgaagat ggggagaagg aagcgagact catacgcaac ctcaatgtca 421 tcttggccaa gtatggtctg gacggaaaga aggacgctcg gcaggtgacc agcaactccc 481 tcagtggcac ccaggaagac gggctggatg accccaggct ggaaaagctg tggcacaagg 541 cgaagacctc tgggaaattc tccggcgaag aactggacaa gctctggcgg gagttcctgc 601 atcacaaaga gaaagttcac gagtacaacg tcctgctgga gaccctgagc aggaccgaag 661 aaatccacga gaacgtcatt agcccctcgg acctgagcga catcaagggc agcgtcctgc 721 acagcaggca cacggagctg aaggagaagc tgcgcagcat caaccagggc ctggaccgcc 781 tgcgcagggt cagccaccag ggctacagca ctgaggctga gttcgaggag cccagggtga 841 ttgacctgtg ggacctggcg cagtccgcca acctcacgga caaggagctg gaggcgttcc 901 gggaggagct caagcacttc GAAGCCAAAA TCGAGAAGCA CAACCACTAC CAGAAGCAGC 961 TGGAGATTGC GCACGAGAAG CTGAGGCACG CAGAGAGCGT GGGCGACGGC GAGCGTGTGA 1021 GCCGCAGCCG CGAGAAGCAC GCCCTGCTGG AGGGGCGGAC CAAGGAGCTG GGCTACACGG 1081 TGAAGAAGCA TCTGCAGGAC CTGTCCGGCA GGATCTCCAG AGCTCGGCAC AACGAACTCT 1141 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1201 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1261 TTTATATATC TTGTGGAAAG GACGATTGAT ACTAAGTGCA TACAAACCAA CGCGTTAAGT 1321 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt