Construct: ORF TRCN0000477225
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004947.1_s317c1
- Derived from:
- ccsbBroadEn_14282
- DNA Barcode:
- ATACCCGATGGGCTAGCAATCACC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- CFAP43 (80217)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477225
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80217 | CFAP43 | cilia and flagella associat... | XM_017016684.1 | 99.8% | 6.9% | 81C>T;196G>T;2730G>A |
| 2 | human | 80217 | CFAP43 | cilia and flagella associat... | XM_011540201.2 | 61.4% | 4.2% | (many diffs) |
| 3 | human | 80217 | CFAP43 | cilia and flagella associat... | XM_011540200.2 | 60.9% | 4.2% | (many diffs) |
| 4 | human | 80217 | CFAP43 | cilia and flagella associat... | XM_005270172.3 | 57.2% | 3.9% | (many diffs) |
| 5 | human | 80217 | CFAP43 | cilia and flagella associat... | XM_005270171.2 | 56.3% | 3.9% | (many diffs) |
| 6 | human | 80217 | CFAP43 | cilia and flagella associat... | NM_025145.6 | 56.2% | 3.9% | (many diffs) |
| 7 | human | 80217 | CFAP43 | cilia and flagella associat... | XM_011540197.2 | 56% | 3.8% | (many diffs) |
| 8 | human | 80217 | CFAP43 | cilia and flagella associat... | XM_011540199.2 | 55.2% | 3.9% | (many diffs) |
| 9 | human | 80217 | CFAP43 | cilia and flagella associat... | XM_011540196.2 | 55% | 3.8% | (many diffs) |
| 10 | human | 80217 | CFAP43 | cilia and flagella associat... | XM_017016681.1 | 54.9% | 3.8% | (many diffs) |
| 11 | human | 80217 | CFAP43 | cilia and flagella associat... | XM_017016682.1 | 54.1% | 4% | (many diffs) |
| 12 | human | 80217 | CFAP43 | cilia and flagella associat... | XM_011540198.2 | 52.7% | 3.9% | (many diffs) |
| 13 | human | 80217 | CFAP43 | cilia and flagella associat... | XM_011540202.2 | 40% | 1.1% | (many diffs) |
| 14 | human | 80217 | CFAP43 | cilia and flagella associat... | XR_002957015.1 | 33.5% | (many diffs) | |
| 15 | human | 80217 | CFAP43 | cilia and flagella associat... | XM_024448177.1 | 23.5% | 1.1% | (many diffs) |
| 16 | human | 80217 | CFAP43 | cilia and flagella associat... | XM_024448178.1 | 11.7% | 1.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 264
- ORF length:
- 195
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgcaaggc cgggagcgcg acgaaggccc ccactccgcc ggcggcgcgt 121 ccttgtccgt gagatgggtg caaggatttc ctaagcagaa tgttcatttt gtcaacgaca 181 acaccatttg ctacccttgt gggaattatg taatatttat taatattgaa accaagaaaa 241 agactgtact gcagtgtagt aattgaattg tgggcgtcat ggcaactaac atcccctgtg 301 aagttgtggc tttttctgac cggaagctaa aacctctcat ctacgtatac agctttccag 361 gattgaccag aaggaccaaa ttgaaaggca acattctcct ggactacact ttactttcat 421 tcagttactg tggcacctac ctggctagtt actcctctct cccagaattt gaactggccc 481 tttggaactg ggaatcgagt atcattttgt gtaagaaatc acagcctgga atggatgtga 541 accaaatgtc ttttaacccc atgaactggc gccagctgtg cttatcaagt ccaagtacag 601 tgagcgtgtg gaccattgaa agaagtaacc aggagcattg tttcagagca aggtcggtga 661 aattacctct agaagatggg tcatttttta atgaaacgga tgtcgttttc ccccagtcgt 721 tgccgaaaga tctcatctat ggtcccgtgc tgccactgtc agccattgcc gggctggtag 781 gcaaagaggc agagactttc cggccgaaag atgatctata tcctttgctt cacccgacta 841 tgcattgctg gactccaaca agtgacttgt acattggctg tgaagagggt catcttttaa 901 tgattaatgg agacaccttg caagtgactg tacttaataa gatagaagag gaatcgccat 961 tggaagacag aagaaatttt atcagtccag taaccttggt atatcagaag gagggcgtgc 1021 tggcttctgg aattgatggc tttgtgtatt cttttattat taaagataga agttacatga 1081 tcgaggattt tcttgagatt gaaagacctg tagaacatat gacattttct cccaattata 1141 cagtgttgct gattcaaaca gacaagggat ctgtttatat ctacactttt ggtaaggagc 1201 caaccttaaa taaagtccta gatgcttgtg atgggaaatt tcaggcaatt gactttatca 1261 cacctggaac ccaatacttc atgacactta catattcagg ggaaatttgt gtttggtggc 1321 tggaggattg tgcttgtgta agcaagattt atctgaatac cctagcaacg gttctggctt 1381 gctgtccatc ctccctctct gcagccgtgg gcacggagga tggctcggtc tacttcatca 1441 gcgtatatga taaggaatcc cctcaggtcg tgcacaaggc ctttctctcg gaatcgtccg 1501 tgcagcacgt cgtttatgat cagcaaggaa tatttctgtt agttggaaca gcagaaggaa 1561 aagtctttat tatcaatgcc aactcctcaa gctcatttca gattattgga ttcacagagg 1621 tggccaaaga cattttacag atatccacag tgtctctttt agaaacagac atagtggaag 1681 tgatggtgct ttcctcgctt ccagaagcag ggagaagcag gttggagatg ttcacactgc 1741 ctacattact gccacaagtt tccacaacct ttgctgatga aagaggaagg ctgaaagatg 1801 aaatcattca taagtacctg tatgagttgg agcacgctct gtcctctgca gtcttgggct 1861 ttcaaagtaa ccaaatatat ggcttctgta gtcaagtgcc atacatctgt agctaccttc 1921 ttcctgaaga agaacatacc ggtatctaca ttcttaaacc atacaaaaaa gtacaaagca 1981 gacagtatgg acctggactg ctctatctgt cttcacatgg attgtggctc ataacaatag 2041 ctaaatgtgg aattctgtgt atccgagacg tttatacttt ggaaacattt gcttggtgtc 2101 ggagtcattc tcaccagggt catgggattc agtcaatgag aatttcaatg gatggacaaa 2161 acattctggt gaatgggaga gatgatggca cccttgtcta cctaaagtgg aagcgatttg 2221 gaggacacct agccagtgaa attctggact attaccagaa actattaatt tccctgagca 2281 gcgccatgga caaggagaat cattatttaa gcacaacacc aaaagtttcc gtagatttgg 2341 gatccgattc tgaacacaca aaacagaagg caagcactga cttatcacaa gatgaattag 2401 ttctaaccga tgttaagaag gaaattcctt ggatacaaca aaaaagccaa gaggccatca 2461 aaaaggaggt taatctgttt tccaagaaaa ggaaagagat aaaacaagga atcaaatcac 2521 tttccaaaac tattctgaat atgatggaag aaaatgacaa actagaaaat attgcaaaat 2581 tagaccaaca ggAATTTGGT CTGGATCTTG AGGAACTAGA AAGGCTTCAT GATGAAAGTC 2641 AGGAAGAAGT GGCAAAGATG ATAAAGGATG TAGAGATGCA TAACTTAGCC AAGAGCTATT 2701 TGGCTGAACT TATCAAAGAA GAATGTTGGA ATTCGATGGC TGTGAAAGGT CGAGCTCTTA 2761 AGTGTTTTCA TATCCCCTGT GTGGTTGAAA ACTTCCCAAT GAAAGCGCGC ACGGTTGAAG 2821 AGCTGAAAGA ATTGGAAAGA GTTTTACAGC AAAAGAAGAT TGAAGCAGAG TGTCTTAAAG 2881 TACTATTTTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 2941 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 3001 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAATACCC GATGGGCTAG CAATCACCAC 3061 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt