Construct: ORF TRCN0000477226

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002567.1_s317c1
Derived from:
ccsbBroadEn_06113
DNA Barcode:
CCTTTCAAGCTCTGCTGCCATAGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DNM2 (1785)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477226
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1785 DNM2 dynamin 2 NM_001005360.2 99.9% 100% 2139T>C
2 human 1785 DNM2 dynamin 2 NM_001190716.2 99.8% 99.8% 2139T>C;2540_2541insCAG
3 human 1785 DNM2 dynamin 2 NM_004945.3 99.5% 99.5% 1544_1545insGGGGGAGATCCT;2127T>C
4 human 1785 DNM2 dynamin 2 NM_001005361.2 98.1% 98.5% (many diffs)
5 human 1785 DNM2 dynamin 2 NM_001005362.2 97.6% 98% (many diffs)
6 mouse 13430 Dnm2 dynamin 2 NM_001253893.1 89.3% 98.1% (many diffs)
7 mouse 13430 Dnm2 dynamin 2 XM_006509979.1 89.2% 98% (many diffs)
8 mouse 13430 Dnm2 dynamin 2 NM_007871.2 88.9% 97.7% (many diffs)
9 mouse 13430 Dnm2 dynamin 2 XM_017313127.1 88.8% 97.5% (many diffs)
10 mouse 13430 Dnm2 dynamin 2 XM_006509973.1 88.3% 97% (many diffs)
11 mouse 13430 Dnm2 dynamin 2 XM_006509977.1 88.3% 96.7% (many diffs)
12 mouse 13430 Dnm2 dynamin 2 XM_006509975.1 88.2% 96.9% (many diffs)
13 mouse 13430 Dnm2 dynamin 2 NM_001039520.2 88.1% 96.6% (many diffs)
14 mouse 13430 Dnm2 dynamin 2 XM_006509976.2 87.9% 96.5% (many diffs)
15 mouse 13430 Dnm2 dynamin 2 XM_006509980.2 87.8% 96.3% (many diffs)
16 mouse 13430 Dnm2 dynamin 2 XM_017313125.1 87.8% 96.4% (many diffs)
17 mouse 13430 Dnm2 dynamin 2 XM_017313128.1 87.7% 96.2% (many diffs)
18 mouse 13430 Dnm2 dynamin 2 NM_001253894.1 87.5% 96.4% (many diffs)
19 mouse 13430 Dnm2 dynamin 2 XM_006509974.1 87.3% 95.6% (many diffs)
20 mouse 13430 Dnm2 dynamin 2 XM_006509982.2 87.1% 95.9% (many diffs)
21 mouse 13430 Dnm2 dynamin 2 XM_006509978.2 86.5% 95.3% (many diffs)
22 mouse 13430 Dnm2 dynamin 2 XM_006509981.2 86.5% 95% (many diffs)
23 mouse 13430 Dnm2 dynamin 2 XM_017313126.1 86.1% 94.8% (many diffs)
24 mouse 13430 Dnm2 dynamin 2 XM_017313129.1 86% 94.5% (many diffs)
25 mouse 13430 Dnm2 dynamin 2 XM_006509983.1 71.6% 79.2% (many diffs)
26 mouse 13430 Dnm2 dynamin 2 XM_006509984.3 52.5% 57% (many diffs)
27 mouse 13430 Dnm2 dynamin 2 XM_006509985.3 46.1% 49.7% (many diffs)
28 mouse 13430 Dnm2 dynamin 2 XR_001778793.1 44.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2676
ORF length:
2610
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggg caaccgcggg atggaagagc tgatcccgct ggtcaacaaa ctgcaggacg
121ccttcagctc catcggccag agctgccacc tggacctgcc gcagatcgct gtagtgggcg
181gccagagcgc cggcaagagc tcggtgctgg agaacttcgt gggccgggac ttccttcccc
241gcggttcagg aatcgtcacc cggcggcctc tcattctgca gctcatcttc tcaaaaacag
301aacatgccga gtttttgcac tgcaagtcca aaaagtttac agactttgat gaagtccggc
361aggagattga agcagagacc gacagggtca cggggaccaa caaaggcatc tccccagtgc
421ccatcaacct tcgagtctac tcgccacacg tgttgaactt gaccctcatc gacctcccgg
481gtatcaccaa ggtgcctgtg ggcgaccagc ctccagacat cgagtaccag atcaaggaca
541tgatcctgca gttcatcagc cgggagagca gcctcattct ggctgtcacg cccgccaaca
601tggacctggc caactccgac gccctcaagc tggccaagga agtcgatccc caaggcctac
661ggaccatcgg tgtcatcacc aagcttgacc tgatggacga gggcaccgac gccagggacg
721tcttggagaa caagttgctc ccgttgagaa gaggctacat tggcgtggtg aaccgcagcc
781agaaggatat tgagggcaag aaggacatcc gtgcagcact ggcagctgag aggaagttct
841tcctctccca cccggcctac cggcacatgg ccgaccgcat gggcacgcca catctgcaga
901agacgctgaa tcagcaactg accaaccaca tccgggagtc gctgccggcc ctacgtagca
961aactacagag ccagctgctg tccctggaga aggaggtgga ggagtacaag aactttcggc
1021ccgacgaccc cacccgcaaa accaaagccc tgctgcagat ggtccagcag tttggggtgg
1081attttgagaa gaggatcgag ggctcaggag atcaggtgga cactctggag ctctccgggg
1141gcgcccgaat caatcgcatc ttccacgagc ggttcccatt tgagctggtg aagatggagt
1201ttgacgagaa ggacttacga cgggagatca gctatgccat taagaacatc catggagtca
1261ggaccgggct tttcaccccg gacttggcat tcgaggccat tgtgaaaaag caggtcgtca
1321agctgaaaga gccctgtctg aaatgtgtcg acctggttat ccaggagcta atcaatacag
1381ttaggcagtg taccagtaag ctcagttcct acccccggtt gcgagaggag acagagcgaa
1441tcgtcaccac ttacatccgg gaacgggagg ggagaacgaa ggaccagatt cttctgctga
1501tcgacattga gcagtcctac atcaacacga accatgagga cttcatcggg tttgccaatg
1561cccagcagag gagcacgcag ctgaacaaga agagagccat ccccaatcag ggggagatcc
1621tggtgatccg caggggctgg ctgaccatca acaacatcag cctgatgaaa ggcggctcca
1681aggagtactg gtttgtgctg actgccgagt cactgtcctg gtacaaggat gaggaggaga
1741aagagaagaa gtacatgctg cctctggaca acctcaagat ccgtgatgtg gagaagggct
1801tcatgtccaa caagcacgtc ttcgccatct tcaacacgga gcagagaaac gtctacaagg
1861acctgcggca gatcgagctg gcctgtgact cccaggaaga cgtggacagc tggaaggcct
1921cgttcctccg agctggcgtc taccccgaga aggaccaggc agaaaacgag gatggggccc
1981aggagaacac cttctccatg gacccccaac tggagcggca ggtggagacc attcgcaacc
2041tggtggactc atacgtggcc atcatcaaca agtccatccg cgacctcatg ccaaagacca
2101tcatgcacct catgatcaac aatacgaagg ccttcatcca ccacgagctg ctggcctacc
2161tatactcctc ggcagaccag agcagcctca tggaggagtc ggccgaccag gcacagcggc
2221gggacgacat gctgcgcatg taccatgccc TCAAGGAGGC GCTCAACATC ATCGGTGACA
2281TCAGCACCAG CACTGTGTCC ACGCCTGTAC CCCCGCCTGT CGATGACACC TGGCTCCAGA
2341GCGCCAGCAG CCACAGCCCC ACTCCACAGC GCCGACCGGT GTCCAGCATA CACCCCCCTG
2401GCCGGCCCCC AGCAGTGAGG GGCCCCACTC CAGGGCCCCC CCTGATTCCT GTTCCCGTGG
2461GGGCAGCAGC CTCCTTCTCG GCGCCCCCAA TCCCATCCCG GCCTGGACCC CAGAGCGTGT
2521TTGCCAACAG TGACCTCTTC CCAGCCCCGC CTCAGATCCC ATCTCGGCCA GTTCGGATCC
2581CCCCAGGGAT TCCCCCAGGA GTGCCCAGCA GAAGACCCCC TGCTGCGCCC AGCCGGCCCA
2641CCATTATCCG CCCAGCCGAG CCATCCCTGC TCGACTACCC AACTTTCTTG TACAAAGTGG
2701TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
2761CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
2821ACCTTTCAAG CTCTGCTGCC ATAGTACGCG TTAAGTCgac aatcaacctc tggattacaa
2881aatttgtgaa agatt

Download FASTA (ORF) (Full)