Construct: ORF TRCN0000477234
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003777.1_s317c1
- Derived from:
- ccsbBroadEn_09051
- DNA Barcode:
- CGGCGCACTATTAAAGCTTTATCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EEPD1 (80820)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477234
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80820 | EEPD1 | endonuclease/exonuclease/ph... | NM_030636.3 | 99.8% | 99.8% | 1028G>A;1644T>C |
| 2 | human | 80820 | EEPD1 | endonuclease/exonuclease/ph... | XM_017012658.2 | 54.9% | 54.8% | (many diffs) |
| 3 | human | 80820 | EEPD1 | endonuclease/exonuclease/ph... | XM_017012657.2 | 54.7% | 51.6% | (many diffs) |
| 4 | mouse | 67484 | Eepd1 | endonuclease/exonuclease/ph... | NM_026189.3 | 85.7% | 90.8% | (many diffs) |
| 5 | mouse | 67484 | Eepd1 | endonuclease/exonuclease/ph... | XM_006510556.3 | 46.5% | 50.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1773
- ORF length:
- 1707
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gagcaccctg ggctgccacc gctccatccc cagggacccc tcggacctgt 121 cccatagccg caagttcagc gcagcctgta acttcagcaa cattctagtg aatcaggagc 181 ggctcaacat caacactgcc acggaggagg agctgatgac cctgcctggg gtgacgcgtg 241 ccgtggcacg cagcatcgtg gagtaccgag agtatatcgg tggcttcaag aaggtggagg 301 acctggcatt ggtcagtggt gtaggcgcca ccaagctgga gcaggtcaag tttgagatct 361 gtgtgagcag caagggcagc tcagcgcagc actctcccag ttccctgcgg cgggacctgc 421 tagcggagca gcagcctcac cacctggcca cagctgtgcc cctcacccca cgtgttaaca 481 tcaacacagc caccccggcc cagctcatga gcgtgcgagg cctctcggag aaaatggccc 541 tcagcatcgt ggacttccgc cgtgagcatg ggccctttcg cagcgttgag gacctagtga 601 ggatggatgg tatcaatgcc gccttcctgg acaggatccg gcaccaggtg tttgctgaga 661 ggtccaggcc cccatccacc cacacgaacg ggggactgac cttcaccgcc aagcctcacc 721 cgagccccac ttccctgagc ctgcagagtg aggacctgga cctgccgcca ggggggccca 781 cccagattat ctccactcgg ccgtccgtgg aggcctttgg aggcacaagg gatgggaggc 841 ctgtgctgag gctggccacc tggaacttgc agggctgttc cgtggagaag gccaacaacc 901 ccggggtgcg agaggtggtg tgcatgacac tcctggaaaa cagcatcaag cttctagctg 961 tgcaagaact gcttgacaga gaggccttgg aaaagttctg cacggagcta aaccagccga 1021 ccctgcccaa catccgcaag tggaaggggc cccggggatg ctggaaggct gttgttgctg 1081 agaagccctc gaatcagctc cagaagggag ctgggtatgc aggattccta tgggacgcgg 1141 ctgccggcat ggagctgaga gacgcgggtt cacaggagag ctcgccaagc aacgggcacg 1201 ggaagctggc gggccccagc ccatacctcg ggaggttcaa ggtgggaagt cacgacctga 1261 cccttgttaa ccttcacctg gcagccctga ccctcctggg gagcgagaat cccagcaaga 1321 atcacagtga tggccaccgg ttggcgagct ttgcacagac cctacaggaa accctgaaag 1381 gagaaaagga tgtcattatc ttaggggatt ttggccaagg gccagacagc aatgactatg 1441 atatCCTGAG GAAAGAAAAG TTCCACCACC TGATCCCCGC GCACACCTTC ACCAACATCA 1501 GCACCAAGAA CCCTCAAGGC TCGAAGTCTC TGGACAACAT CTGGATCAGT AAAAGCTTAA 1561 AGAAGGTTTT CACAGGTCAC TGGGCTGTGG TGAGAGAAGG CCTCACGAAC CCTTGGATTC 1621 CGGATAACTG GTCTTGGGGC GGGGTGGCTT CTGAACACTG CCCAGTGCTA GCCGAGTTCT 1681 ACACTGAAAA GGACTGGAGC AAGAAGGACG CCCCTCGGAA CGGCAGCGGG GTGGCCTTGG 1741 AGCGAAGTGA AGCCAACATC AAGCACGAGC GATGCCCAAC TTTCTTGTAC AAAGTGGTTG 1801 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1861 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACG 1921 GCGCACTATT AAAGCTTTAT CGACGCGTTA AGTCgacaat caacctctgg attacaaaat 1981 ttgtgaaaga tt