Construct: ORF TRCN0000477263
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014835.1_s317c1
- Derived from:
- ccsbBroadEn_00474
- DNA Barcode:
- CTCTAGGTACCCCCTCCATGTCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ECT2 (1894)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477263
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1894 | ECT2 | epithelial cell transforming 2 | NM_001258316.1 | 100% | 100% | |
| 2 | human | 1894 | ECT2 | epithelial cell transforming 2 | NM_018098.6 | 100% | 100% | |
| 3 | human | 1894 | ECT2 | epithelial cell transforming 2 | NM_001349102.2 | 99.8% | 99.8% | 125_126insAGA |
| 4 | human | 1894 | ECT2 | epithelial cell transforming 2 | NM_001349103.2 | 96.7% | 96.6% | 1111_1112ins87 |
| 5 | human | 1894 | ECT2 | epithelial cell transforming 2 | NM_001349104.2 | 96.7% | 96.6% | 1111_1112ins87 |
| 6 | human | 1894 | ECT2 | epithelial cell transforming 2 | NM_001258315.2 | 96.6% | 96.6% | 210_302del |
| 7 | human | 1894 | ECT2 | epithelial cell transforming 2 | NM_001349098.2 | 96.6% | 96.6% | 210_302del |
| 8 | human | 1894 | ECT2 | epithelial cell transforming 2 | NM_001349099.2 | 96.6% | 96.6% | 210_302del |
| 9 | human | 1894 | ECT2 | epithelial cell transforming 2 | NM_001349100.2 | 96.6% | 96.6% | 210_302del |
| 10 | human | 1894 | ECT2 | epithelial cell transforming 2 | NM_001349101.2 | 94.3% | 91.5% | (many diffs) |
| 11 | human | 1894 | ECT2 | epithelial cell transforming 2 | XM_005247176.2 | 94.3% | 91.5% | (many diffs) |
| 12 | human | 1894 | ECT2 | epithelial cell transforming 2 | XM_011512516.1 | 94.3% | 91.5% | (many diffs) |
| 13 | human | 1894 | ECT2 | epithelial cell transforming 2 | XM_017005832.1 | 94.3% | 91.5% | (many diffs) |
| 14 | human | 1894 | ECT2 | epithelial cell transforming 2 | XM_006713524.4 | 93.2% | 93.2% | (many diffs) |
| 15 | human | 1894 | ECT2 | epithelial cell transforming 2 | NM_001349094.2 | 91.3% | 88.6% | (many diffs) |
| 16 | human | 1894 | ECT2 | epithelial cell transforming 2 | NM_001349095.2 | 91.3% | 88.6% | (many diffs) |
| 17 | human | 1894 | ECT2 | epithelial cell transforming 2 | NM_001349096.2 | 91.3% | 88.6% | (many diffs) |
| 18 | human | 1894 | ECT2 | epithelial cell transforming 2 | XM_011512514.2 | 91.3% | 88.6% | (many diffs) |
| 19 | human | 1894 | ECT2 | epithelial cell transforming 2 | NM_001349097.2 | 91.2% | 88.5% | (many diffs) |
| 20 | human | 1894 | ECT2 | epithelial cell transforming 2 | XM_017005831.1 | 88.3% | 85.5% | (many diffs) |
| 21 | mouse | 13605 | Ect2 | ect2 oncogene | NM_001177625.1 | 86.8% | 91.1% | (many diffs) |
| 22 | mouse | 13605 | Ect2 | ect2 oncogene | NM_001177626.1 | 86.8% | 91.1% | (many diffs) |
| 23 | mouse | 13605 | Ect2 | ect2 oncogene | NM_007900.3 | 83.8% | 88% | (many diffs) |
| 24 | mouse | 13605 | Ect2 | ect2 oncogene | XM_006535385.3 | 83.8% | 88% | (many diffs) |
| 25 | mouse | 13605 | Ect2 | ect2 oncogene | XM_006535386.3 | 83.8% | 88% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2718
- ORF length:
- 2649
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctgaaaat agtgtattaa catccactac tgggaggact agcttggcag 121 actcttccat ttttgattct aaagttactg agatttccaa ggaaaactta cttattggat 181 ctacttcata tgtagaagaa gagatgcctc agattgaaac aagagtgata ttggttcaag 241 aagctggaaa acaagaagaa cttataaaag ccttaaagga cattaaagtg ggctttgtaa 301 agatggagtc agtggaagaa tttgaaggtt tggattctcc ggaatttgaa aatgtatttg 361 tagtcacgga ctttcaggat tctgtcttta atgacctcta caaggctgat tgtagagtta 421 ttggaccacc agttgtatta aattgttcac aaaaaggaga gcctttgcca ttttcatgtc 481 gcccgttgta ttgtacaagt atgatgaatc tagtactatg ctttactgga tttaggaaaa 541 aagaagaact agtcaggttg gtgacattgg tccatcacat gggtggagtt attcgaaaag 601 actttaattc aaaagttaca catttggtgg caaattgtac acaaggagaa aaattcaggg 661 ttgctgtgag tctaggtact ccaattatga agccagaatg gatttataaa gcttgggaaa 721 ggcggaatga acaggatttc tatgcagcag ttgatgactt tagaaatgaa tttaaagttc 781 ctccatttca agattgtatt ttaagtttcc tgggattttc agatgaagag aaaaccaata 841 tggaagaaat gactgaaatg caaggaggta aatatttacc gcttggagat gaaagatgca 901 ctcaccttgt agttgaagag aatatagtaa aagatcttcc ctttgaacct tcaaagaaac 961 tttatgttgt caagcaagag tggttctggg gaagcattca aatggatgcc cgagctggag 1021 aaactatgta tttatatgaa aaggcaaata ctcctgagct caagaaatca gtgtcaatgc 1081 tttctctaaa tacccctaac agcaatcgca aacgacgtcg tttaaaagaa acacttgctc 1141 agctttcaag agagacagac gtgtcaccat ttccaccccg taagcgccca tcagctgagc 1201 attccctttc catagggtca ctcctagata tctccaacac accagagtct agcattaact 1261 atggagacac cccaaagtct tgtactaagt cttctaaaag ctccactcca gttccttcaa 1321 agcagtcagc aaggtggcaa gttgcaaaag agctttatca aactgaaagt aattatgtta 1381 atatattggc aacaattatt cagttatttc aagtaccatt ggaagaggaa ggacaacgtg 1441 gtggacctat ccttgcacca gaggagatta agactatttt tggtagcatc ccagatatct 1501 ttgatgtaca cactaagata aaggatgatc ttgaagacct tatagttaat tgggatgaga 1561 gcaaaagcat tggtgacatt tttctgaaat attcaaaaga tttggtaaaa acctaccctc 1621 cctttgtaaa cttctttgaa atgagcaagg aaacaattat taaatgtgaa aaacagaaac 1681 caagatttca tgcttttctc aagataaacc aagcaaaacc agaatgtgga cggcagagcc 1741 ttgttgaact tcttatccga ccagtacaga ggttacccag tgttgcatta cttttaaatg 1801 atcttaagaa gcatacagct gatgaaaatc cagacaaaag cactttagaa aaagctattg 1861 gatcactgaa ggaagtaatg acgcatatta atgaggataa gagaaaaaca gaagctcaaa 1921 agcaaatttt tgatgttgtt tatgaagtag atggatgccc agctaatctt ttatcttctc 1981 accgaagctt agtacagcgg gttgaaacaa tttctctagg tgagcacccc tgtgacagag 2041 gagaacaagt aactctcttc ctcttcaatg attgcctaga gatagcaaga aaacggcaca 2101 aggttattgg cacttttagg agtcctcatg gccaaacccg acccccagct tctcttaagc 2161 atattcacct aatgcctctt tctcagatta agaaggtatt ggacataaga gagacagaag 2221 attgccataa tgcttttgcc ttgcttgtga ggCCACCAAC AGAGCAGGCA AATGTGCTAC 2281 TCAGTTTCCA GATGACATCA GATGAACTTC CAAAAGAAAA CTGGCTAAAG ATGCTGTGTC 2341 GACATGTAGC TAACACCATT TGTAAAGCAG ATGCTGAGAA TCTTATTTAT ACTGCTGATC 2401 CAGAATCCTT TGAAGTAAAT ACAAAAGATA TGGACAGTAC ATTGAGTAGA GCATCAAGAG 2461 CAATAAAAAA GACTTCAAAA AAGGTTACAA GAGCATTCTC TTTCTCCAAA ACTCCAAAAA 2521 GAGCTCTTCG AAGGGCTCTT ATGACATCCC ACGGCTCAGT GGAGGGAAGA AGTCCTTCCA 2581 GCAATGATAA GCATGTAATG AGTCGTCTTT CTAGCACATC ATCATTAGCA GGTATCCCTT 2641 CTCCCTCCCT TGTCAGCCTT CCTTCCTTCT TTGAAAGGAG AAGTCATACG TTAAGTAGAT 2701 CTACAACTCA TTTGATATTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 2761 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 2821 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACTCTAGG TACCCCCTCC 2881 ATGTCCCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt