Transcript: Mouse XM_006535386.3

PREDICTED: Mus musculus ect2 oncogene (Ect2), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Ect2 (13605)
Length:
3936
CDS:
310..3051

Additional Resources:

NCBI RefSeq record:
XM_006535386.3
NBCI Gene record:
Ect2 (13605)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006535386.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000353445 GAAAGCGGCACAAGGTTATTG pLKO_005 2423 CDS 100% 13.200 10.560 N Ect2 n/a
2 TRCN0000216338 GCCACAATCATTCAATTATTT pLKO.1 1723 CDS 100% 15.000 10.500 N Ect2 n/a
3 TRCN0000336497 ACCAGACTGAGAGCAATTATG pLKO_005 1691 CDS 100% 13.200 9.240 N Ect2 n/a
4 TRCN0000216416 GAGCAAGGAAATGATCATTAA pLKO.1 1977 CDS 100% 13.200 9.240 N Ect2 n/a
5 TRCN0000191475 GCAGTTGATGACTTTAGAAAT pLKO.1 1081 CDS 100% 13.200 9.240 N Ect2 n/a
6 TRCN0000336437 GCAGTTGATGACTTTAGAAAT pLKO_005 1081 CDS 100% 13.200 9.240 N Ect2 n/a
7 TRCN0000336440 TCGATTGTTTGATGTACTTAA pLKO_005 3467 3UTR 100% 13.200 9.240 N Ect2 n/a
8 TRCN0000336496 TGAGCAAGGAAATGATCATTA pLKO_005 1976 CDS 100% 13.200 9.240 N Ect2 n/a
9 TRCN0000215378 CTCAAGATAAATCAAGCTAAA pLKO.1 2032 CDS 100% 10.800 7.560 N Ect2 n/a
10 TRCN0000201945 CGTGCAGGAGAGACTATGTAT pLKO.1 1345 CDS 100% 5.625 3.938 N Ect2 n/a
11 TRCN0000190717 GCCCAGCTAATCTCTTATCTT pLKO.1 2291 CDS 100% 5.625 3.938 N Ect2 n/a
12 TRCN0000190976 CCCTCCGTTTGTAAACTTCTT pLKO.1 1950 CDS 100% 4.950 3.465 N Ect2 n/a
13 TRCN0000201874 GCAATGCAACACTTGCTGAAT pLKO.1 3410 3UTR 100% 4.950 3.465 N Ect2 n/a
14 TRCN0000190489 GCACAAGGTTATTGGCACTTT pLKO.1 2430 CDS 100% 4.950 3.465 N Ect2 n/a
15 TRCN0000217720 GAAAGGTGCACTCACCTTATT pLKO.1 1225 CDS 100% 1.320 0.924 N Ect2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006535386.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00474 pDONR223 100% 83.8% 88% None (many diffs) n/a
2 ccsbBroad304_00474 pLX_304 0% 83.8% 88% V5 (many diffs) n/a
3 TRCN0000477263 CTCTAGGTACCCCCTCCATGTCCC pLX_317 17.8% 83.8% 88% V5 (many diffs) n/a
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