Construct: ORF TRCN0000477267
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004629.1_s317c1
- Derived from:
- ccsbBroadEn_06284
- DNA Barcode:
- AATTTGTACTGATGTGACGGTCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GLRA1 (2741)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477267
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2741 | GLRA1 | glycine receptor alpha 1 | NM_001146040.2 | 99.8% | 99.7% | 90C>A;638C>A |
| 2 | human | 2741 | GLRA1 | glycine receptor alpha 1 | NM_000171.4 | 98.1% | 98% | 90C>A;638C>A;1057_1058ins24 |
| 3 | human | 2741 | GLRA1 | glycine receptor alpha 1 | NM_001292000.2 | 80% | 79.8% | 0_1ins249;389C>A;808_809ins24 |
| 4 | mouse | 14654 | Glra1 | glycine receptor, alpha 1 s... | NM_001290821.1 | 90.5% | 98.6% | (many diffs) |
| 5 | mouse | 14654 | Glra1 | glycine receptor, alpha 1 s... | NM_020492.4 | 89% | 96.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1440
- ORF length:
- 1371
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtacagcttc aatactcttc gactctacct ttgggagacc attgtattct 121 tcagccttgc tgcttctaag gaggctgaag ctgctcgatc cgcacccaag cctatgtcac 181 cctcggattt cctggataag ctaatgggga gaacctccgg atatgatgcc aggatcaggc 241 ccaattttaa aggtccccca gtgaacgtga gctgcaacat tttcatcaac agctttggtt 301 ccattgctga gacaaccatg gactataggg tcaacatctt cctgcggcag caatggaacg 361 acccccgcct ggcctataat gaataccctg acgactctct ggacctggac ccatccatgc 421 tggactccat ctggaaacct gacctgttct ttgccaacga gaagggggcc cacttccatg 481 agatcaccac agacaacaaa ttgctaagga tctcccggaa tgggaatgtc ctctacagca 541 tcagaatcac cctgacactg gcctgcccca tggacttgaa gaatttcccc atggatgtcc 601 agacatgtat catgcaactg gaaagctttg gatatacgat gaatgacctc atctttgagt 661 ggcaggaaca gggagccgtg caggtagcag atggactaac tctgcaccag tttatcttga 721 aggaagagaa ggacttgaga tactgcacca agcactacaa cacaggtaaa ttcacctgca 781 ttgaggcccg gttccacctg gagcggcaga tgggttacta cctgattcag atgtatattc 841 ccagcctgct cattgtcatc ctctcatgga tctccttctg gatcaacatg gatgctgcac 901 ctgctcgtgt gggcctaggc atcaccactg tgctcaccat gaccacccag agctccggct 961 ctcgagcatc tctgcccaag gtgtcctatg tgaaagccat tgacatttgg atggcagttt 1021 gcctgctctt tgtgttctca gccctattag aatatgctgc cgttaacttt gtgtctcggc 1081 aacataagga gctgctccga ttcaggagga agcggagaca tcacaagagc cccatgttga 1141 atctattcca ggaggatgaa gctggagaag gccgctttaa cttctctgcc tatgggatgg 1201 gcccagcctg tctacaggcc aaggatggca tcTCAGTCAA GGGCGCCAAC AACAGTAACA 1261 CCACCAACCC CCCTCCTGCA CCATCTAAGT CCCCAGAGGA GATGCGAAAA CTCTTCATCC 1321 AGAGGGCCAA GAAGATCGAC AAAATATCCC GCATTGGCTT CCCCATGGCC TTCCTCATTT 1381 TCAACATGTT CTACTGGATC ATCTACAAGA TTGTCCGTAG AGAGGACGTC CACAACCAGT 1441 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1501 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1561 TTTATATATC TTGTGGAAAG GACGAAATTT GTACTGATGT GACGGTCAGA CGCGTTAAGT 1621 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt