Construct: ORF TRCN0000477297
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005854.1_s317c1
- Derived from:
- ccsbBroadEn_09516
- DNA Barcode:
- CGCTATTAATCCAAAAGGAAGCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CSTL1 (128817)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477297
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 128817 | CSTL1 | cystatin like 1 | NM_138283.1 | 99.7% | 99.3% | 262T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 504
- ORF length:
- 435
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggggatcgga tgctggagaa accccctgct gctgctgatt gccctggtcc 121 tgtcagccaa gctgggtcac ttccaaaggt gggagggctt ccagcagaag ctcatgagca 181 agaagaacat gaattcaaca ctcaacttct tcattcaatc cTACAACAAT GCCAGCAACG 241 ACACCTACTT ATATCGAGTC CAGAGGCTAA TTCGAAGTCA GATGCAGCTG ACGACGGGAG 301 TGGAGTATAT AGTCACTGTG AAGATTGGCC GGACCAAATG CAAGAGGAAT GACACGAGCA 361 ATTCTTCCTG CCCCCTGCAA AGCAAGAAGC TGAGAAAGAG TTTAATTTGC GAGTCTTTGA 421 TATACACCAT GCCCTGGATA AACTATTTCC AGCTCTGGAA CAATTCCTGT CTGGAGGCCG 481 AGCATGTGGG CAGAAACCTC AGATTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 541 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 601 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC GCTATTAATC 661 CAAAAGGAAG CGGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 721 att