Construct: ORF TRCN0000477298
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002640.1_s317c1
- Derived from:
- ccsbBroadEn_16164
- DNA Barcode:
- CGAGCATGCTTGTTAGAGTCCTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SCIMP (388325)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477298
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 388325 | SCIMP | SLP adaptor and CSK interac... | NM_207103.3 | 99.5% | 100% | 84C>T;99T>C |
| 2 | human | 388325 | SCIMP | SLP adaptor and CSK interac... | NM_001271842.1 | 94.7% | 95.1% | 84C>T;99T>C;124_125ins21 |
| 3 | human | 388325 | SCIMP | SLP adaptor and CSK interac... | NM_001319190.1 | 65.8% | 54.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 504
- ORF length:
- 435
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttGGCACCAT GGATACTTTC ACAGTTCAGG ATTCCACTGC AATGAGCTGG TGGAGGAATA 121 ATTTCTGGAT CATCTTAGCT GTGGCCATCA TTGTTGTCTC TGTGGGCCTG GGCCTCATCC 181 TGTACTGTGT CTGTAAGTGG CAGCTTAGAC GAGGCAAGAA ATGGGAAATT GCCAAGCCCC 241 TGAAACACAA GCAAGTAGAT GAAGAAAAGA TGTATGAGAA TGTTCTTAAT GAGTCGCCAG 301 TTCAATTACC GCCTCTGCCA CCGAGGAATT GGCCTTCTCT AGAAGACTCT TCCCCACAGG 361 AAGCCCCAAG TCAGCCGCCC GCTACATACT CACTGGTAAA TAAAGTTAAA AATAAGAAGA 421 CTGTTTCCAT CCCAAGCTAC ATTGAGCCTG AAGATGACTA TGACGATGTT GAAATCCCTG 481 CAAATACTGA AAAAGCATCA TTTTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 541 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 601 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC GAGCATGCTT 661 GTTAGAGTCC TGTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 721 att