Construct: ORF TRCN0000477318
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010765.1_s317c1
- Derived from:
- ccsbBroadEn_13283
- DNA Barcode:
- CCGTACAGCATCACTATAATTTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- XXYLT1 (152002)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477318
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 152002 | XXYLT1 | xyloside xylosyltransferase 1 | NM_152531.5 | 96.6% | 96.6% | 1_39del |
| 2 | human | 152002 | XXYLT1 | xyloside xylosyltransferase 1 | XM_017005749.2 | 63.3% | 63.3% | 1_39del;786_787ins393 |
| 3 | human | 152002 | XXYLT1 | xyloside xylosyltransferase 1 | NM_001308069.1 | 62.4% | 59.1% | (many diffs) |
| 4 | human | 152002 | XXYLT1 | xyloside xylosyltransferase 1 | XR_924105.3 | 55.1% | (many diffs) | |
| 5 | human | 152002 | XXYLT1 | xyloside xylosyltransferase 1 | XM_005269286.5 | 54.2% | 54.2% | 0_1ins522 |
| 6 | human | 152002 | XXYLT1 | xyloside xylosyltransferase 1 | XM_011512448.3 | 54.2% | 54.2% | 0_1ins522 |
| 7 | human | 152002 | XXYLT1 | xyloside xylosyltransferase 1 | XM_017005750.2 | 49.5% | 45.3% | (many diffs) |
| 8 | mouse | 268880 | Xxylt1 | xyloside xylosyltransferase 1 | NM_198626.2 | 87.2% | 89.8% | (many diffs) |
| 9 | mouse | 268880 | Xxylt1 | xyloside xylosyltransferase 1 | XM_006522200.2 | 48.2% | 50.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1206
- ORF length:
- 1140
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gcgcctgggc gctgtgcgct cccactactg cgccctgctg ctggccgcgg 121 cgctggccgt ctgcgccttc tactacctcg gctcaggccg ggagaccttc tccagcgcca 181 ccaagaggct gaaggaggcc cgcgccgggg ctcccgccgc gccctcgccg cccgcgctgg 241 agctagcgcg gggctccgtg gcgccagccc ccggcgcgaa ggccaagagc ttggagggcg 301 gcggtgccgg gccggtggac taccacctgc tgatgatgtt caccaaggcg gagcacaatg 361 ccgcgctgca ggccaaggcc cgcgtcgcgc tgcgctcact gctgcgcctc gccaagttcg 421 aggcgcacga ggtgcttaac cttcacttcg tgagcgagga ggccagccgc gaggtggcca 481 agggcctgct gcgggagctc ctgccgcccg ccgctggctt caagtgcaag gtcatcttcc 541 acgatgttgc tgtgctgacg gataagctct tccccatcgt ggaggccatg cagaagcact 601 tcagtgctgg cttgggaacc tactacagtg actccatctt cttcctctcg gtcgccatgc 661 atcagatcat gcccaaagag atcctgcaga tcattcagct ggacctagac ctgaagttta 721 agaccaacat ccgggagttg tttgaggaat ttgacagttt cctgccaggc gccatcatcg 781 gcatagcccg ggagatgcag ccagtttaca ggcacacatT CTGGCAGTTC CGCCATGAGA 841 ACCCCCAGAC CCGGGTTGGG GGCCCGCCCC CCGAGGGGCT GCCGGGCTTC AACAGCGGGG 901 TGATGTTGCT GAACCTGGAG GCCATGCGCC AGTCCCCGCT CTACAGCCGC CTGCTGGAGC 961 CGGCGCAGGT GCAGCAGCTG GCCGACAAGT ACCACTTCCG CGGCCACCTC GGGGACCAGG 1021 ACTTCTTCAC CATGATCGGC ATGGAGCACC CCAAGCTCTT CCATGTGCTG GACTGTACCT 1081 GGAACCGGCA GCTGTGCACC TGGTGGAGGG ACCATGGCTA CAGTGACGTC TTCGAGGCCT 1141 ATTTCAGGTG TGAGGGCCAC GTCAAGATCT ACCACGGGAA CTGCAACACT CCCATCCCGG 1201 AGGACTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1261 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1321 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACCGTACAGC ATCACTATAA TTTTGACGCG 1381 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt