Construct: ORF TRCN0000477377
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017628.1_s317c1
- Derived from:
- ccsbBroadEn_08582
- DNA Barcode:
- GATACTTTAGACAAAGATTTATGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EXOC1 (55763)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477377
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55763 | EXOC1 | exocyst complex component 1 | NM_001024924.2 | 99.9% | 100% | 228T>G |
| 2 | human | 55763 | EXOC1 | exocyst complex component 1 | NM_018261.3 | 99.9% | 100% | 228T>G |
| 3 | human | 55763 | EXOC1 | exocyst complex component 1 | NM_178237.2 | 98.2% | 98.2% | 228T>G;1330_1331ins45 |
| 4 | human | 55763 | EXOC1 | exocyst complex component 1 | XM_005265750.3 | 98.2% | 98.2% | 228T>G;1330_1331ins45 |
| 5 | human | 55763 | EXOC1 | exocyst complex component 1 | XM_005265747.3 | 97.9% | 98% | 228T>G;1075_1128del |
| 6 | human | 55763 | EXOC1 | exocyst complex component 1 | XM_005265748.1 | 97.9% | 98% | 228T>G;1075_1128del |
| 7 | human | 55763 | EXOC1 | exocyst complex component 1 | XM_017008405.2 | 96.3% | 96.2% | 228T>G;1075_1128del;1384_1385ins45 |
| 8 | human | 55763 | EXOC1 | exocyst complex component 1 | XM_017008406.1 | 96.3% | 96.2% | 228T>G;1075_1128del;1384_1385ins45 |
| 9 | human | 55763 | EXOC1 | exocyst complex component 1 | XR_001741286.1 | 75.9% | (many diffs) | |
| 10 | human | 55763 | EXOC1 | exocyst complex component 1 | XR_001741285.2 | 71.8% | (many diffs) | |
| 11 | human | 55763 | EXOC1 | exocyst complex component 1 | XM_017008407.2 | 60.1% | 60.1% | (many diffs) |
| 12 | human | 55763 | EXOC1 | exocyst complex component 1 | XM_017008408.2 | 56.2% | 56.2% | 0_1ins1173 |
| 13 | human | 55763 | EXOC1 | exocyst complex component 1 | XM_017008409.1 | 54.5% | 54.4% | 0_1ins1173;157_158ins45 |
| 14 | mouse | 69940 | Exoc1 | exocyst complex component 1 | NM_001289770.1 | 88.4% | 97.2% | (many diffs) |
| 15 | mouse | 69940 | Exoc1 | exocyst complex component 1 | NM_027270.2 | 87.7% | 96.4% | (many diffs) |
| 16 | mouse | 69940 | Exoc1 | exocyst complex component 1 | NM_001289771.1 | 86.8% | 95.4% | (many diffs) |
| 17 | mouse | 69940 | Exoc1 | exocyst complex component 1 | XM_006535212.2 | 86.1% | 94.6% | (many diffs) |
| 18 | mouse | 69940 | Exoc1 | exocyst complex component 1 | XM_006535214.2 | 51.4% | 55.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2751
- ORF length:
- 2682
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gacagcaatc aagcatgcat tacaaagaga catttttaca ccaaatgatg 121 aacgcctgct gagcattgtg aatgtctgca aagcaggaaa aaagaaaaag aactgttttt 181 tatgtgccac agtgacaact gaacgccctg tgcaggttaa ggtggtcaaa gtcaagaaat 241 ccgataaggg agatttctac aaaaggcaga ttgcatgggc ccttcgagat cttgcggtgg 301 tagatgccaa agatgctatc aaagaaaatc ctgaatttga tttacacttt gaaaaaatat 361 ataaatgggt tgccagcagc actgctgaaa agaatgcatt tatttcatgc atttggaaat 421 tgaatcagcg atatctccgg aagaaaattg attttgtcaa tgttagctca cagcttttgg 481 aagaatctgt tccaagtgga gaaaatcaga gtgtgacagg aggtgatgaa gaagtagtag 541 atgaatacca agagttaaat gcaagagaag aacaggatat cgaaataatg atggaaggct 601 gtgaatatgc aatctcgaat gcggaagcct ttgcagaaaa attgtccaga gagctgcagg 661 tgctagatgg ggctaacatc cagtcaatca tggcatctga aaaacaagtc aacatcctga 721 tgaaattgct agatgaggct ctaaaggagg tagatcagat tgaattgaaa ctgagcagtt 781 atgaggaaat gctccaaagt gtaaaagaac aaatggatca gatctctgaa agcaaccacc 841 taattcatct tagtaacact aataatgtaa aactcctatc tgagatagag ttccttgtga 901 accacatgga cttggccaaa ggtcatataa aggcccttca ggaaggagat cttgcttctt 961 ccagaggcat tgaggcctgc accaatgctg ctgatgccct tctgcagtgc atgaatgtag 1021 ctcttcgacc aggccatgac ttgcttctgg cagtcaaaca gcaacagcag cgattcagtg 1081 atttgcgaga gctttttgcc cggagactgg ccagtcacct caacaatgtt tttgttcaac 1141 agggtcatga tcagagttcg actcttgccc aacactctgt tgaactgact ttacccaatc 1201 atcatccatt tcatagagat ttgctccgat atgccaagct gatggagtgg ctaaagagta 1261 cagattatgg aaaatatgaa ggactaacaa agaattacat ggattattta tcccgactat 1321 atgaaagaga aatcaaagat ttctttgaag ttgcaaagat caagatgact ggcacaacta 1381 aagaaagcaa gaagtttgct acactgcctc gaaaagaaag tgctgtcaaa caggaaacag 1441 agagtcttca tggaagttcg gggaaattaa ctggatctac ttctagtcta aataagctca 1501 gtgttcagag ttcagggaat cgcagatctc agtcatcttc cctgttggat atgggaaaca 1561 tgtctgcctc tgatctcgat gttgctgaca ggaccaaatt tgataagatc tttgaacagg 1621 tactaagtga actggagccc ctatgtctgg cagaacagga cttcataagt aaatttttca 1681 aactacagca acatcaaagt atgcctggaa ctatggctga agcagaggac ctggatggag 1741 gaacattatc acggcaacat aattgtggca caccactgcc tgtttcatct gagaaagata 1801 tgatccgcca aatgatgatt aaaatatttc gctgcattga gccagagctg aacaacctaa 1861 ttgcattagg agacaaaatt gatagcttta actctcttta tatgttagtc aaaatgagtc 1921 atcatgtgtg gactgcacaa aatgtggacc ctgcttcttt cctaagtact acattgggaa 1981 atgttttggt gactgtcaaa aggaactttg acaaatgcat tagtaaccaa ataaggcaaa 2041 tggaagaagt aaagatctca aaaaagagta aagttggaat tcttccattt gttgctgaat 2101 ttgaagaatt tgctggactt gcagaatcaa tcttcaaaaa tgctgagcgt cgtggagacc 2161 tggataaagc atacaccaaa cttatcagag gagtatttgt taatgtggag aaagtagcaa 2221 atgaaagcca gaagaccccc agggatgtgg ttatgatgga aaactttcac catatttttg 2281 caactctttc tcgattgaaa atctcatgtc tagaagcaga aaaaaaagaa gccaaacaaa 2341 aatacacaga tcaccttcag tcttatgtca tttactcttt aggacaacct cttGAAAAAC 2401 TAAATCATTT CTTTGAAGGT GTTGAAGCTC GCGTGGCACA GGGCATAAGG GAGGAGGAAG 2461 TAAGTTACCA ACTTGCATTT AACAAACAAG AACTTCGTAA AGTCATTAAG GAGTACCCTG 2521 GAAAGGAAGT AAAAAAAGGT CTAGATAACC TCTACAAGAA AGTTGATAAA CATTTATGTG 2581 AAGAAGAGAA CTTACTTCAG GTGGTGTGGC ACTCCATGCA AGATGAATTT ATACGCCAGT 2641 ATAAGCACTT TGAAGGTTTG ATAGCTCGCT GTTATCCTGG ATCTGGTGTT ACAATGGAAT 2701 TCACTATTCA GGACATTCTG GATTATTGTT CCAGCATTGC ACAGTCCCAC TTGCCAACTT 2761 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2821 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2881 CTTGTGGAAA GGACGAGATA CTTTAGACAA AGATTTATGT ACGCGTTAAG TCgacaatca 2941 acctctggat tacaaaattt gtgaaagatt