Construct: ORF TRCN0000477377

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017628.1_s317c1
Derived from:
ccsbBroadEn_08582
DNA Barcode:
GATACTTTAGACAAAGATTTATGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EXOC1 (55763)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477377
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55763 EXOC1 exocyst complex component 1 NM_001024924.2 99.9% 100% 228T>G
2 human 55763 EXOC1 exocyst complex component 1 NM_018261.3 99.9% 100% 228T>G
3 human 55763 EXOC1 exocyst complex component 1 NM_178237.2 98.2% 98.2% 228T>G;1330_1331ins45
4 human 55763 EXOC1 exocyst complex component 1 XM_005265750.3 98.2% 98.2% 228T>G;1330_1331ins45
5 human 55763 EXOC1 exocyst complex component 1 XM_005265747.3 97.9% 98% 228T>G;1075_1128del
6 human 55763 EXOC1 exocyst complex component 1 XM_005265748.1 97.9% 98% 228T>G;1075_1128del
7 human 55763 EXOC1 exocyst complex component 1 XM_017008405.2 96.3% 96.2% 228T>G;1075_1128del;1384_1385ins45
8 human 55763 EXOC1 exocyst complex component 1 XM_017008406.1 96.3% 96.2% 228T>G;1075_1128del;1384_1385ins45
9 human 55763 EXOC1 exocyst complex component 1 XR_001741286.1 75.9% (many diffs)
10 human 55763 EXOC1 exocyst complex component 1 XR_001741285.2 71.8% (many diffs)
11 human 55763 EXOC1 exocyst complex component 1 XM_017008407.2 60.1% 60.1% (many diffs)
12 human 55763 EXOC1 exocyst complex component 1 XM_017008408.2 56.2% 56.2% 0_1ins1173
13 human 55763 EXOC1 exocyst complex component 1 XM_017008409.1 54.5% 54.4% 0_1ins1173;157_158ins45
14 mouse 69940 Exoc1 exocyst complex component 1 NM_001289770.1 88.4% 97.2% (many diffs)
15 mouse 69940 Exoc1 exocyst complex component 1 NM_027270.2 87.7% 96.4% (many diffs)
16 mouse 69940 Exoc1 exocyst complex component 1 NM_001289771.1 86.8% 95.4% (many diffs)
17 mouse 69940 Exoc1 exocyst complex component 1 XM_006535212.2 86.1% 94.6% (many diffs)
18 mouse 69940 Exoc1 exocyst complex component 1 XM_006535214.2 51.4% 55.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2751
ORF length:
2682
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gacagcaatc aagcatgcat tacaaagaga catttttaca ccaaatgatg
121aacgcctgct gagcattgtg aatgtctgca aagcaggaaa aaagaaaaag aactgttttt
181tatgtgccac agtgacaact gaacgccctg tgcaggttaa ggtggtcaaa gtcaagaaat
241ccgataaggg agatttctac aaaaggcaga ttgcatgggc ccttcgagat cttgcggtgg
301tagatgccaa agatgctatc aaagaaaatc ctgaatttga tttacacttt gaaaaaatat
361ataaatgggt tgccagcagc actgctgaaa agaatgcatt tatttcatgc atttggaaat
421tgaatcagcg atatctccgg aagaaaattg attttgtcaa tgttagctca cagcttttgg
481aagaatctgt tccaagtgga gaaaatcaga gtgtgacagg aggtgatgaa gaagtagtag
541atgaatacca agagttaaat gcaagagaag aacaggatat cgaaataatg atggaaggct
601gtgaatatgc aatctcgaat gcggaagcct ttgcagaaaa attgtccaga gagctgcagg
661tgctagatgg ggctaacatc cagtcaatca tggcatctga aaaacaagtc aacatcctga
721tgaaattgct agatgaggct ctaaaggagg tagatcagat tgaattgaaa ctgagcagtt
781atgaggaaat gctccaaagt gtaaaagaac aaatggatca gatctctgaa agcaaccacc
841taattcatct tagtaacact aataatgtaa aactcctatc tgagatagag ttccttgtga
901accacatgga cttggccaaa ggtcatataa aggcccttca ggaaggagat cttgcttctt
961ccagaggcat tgaggcctgc accaatgctg ctgatgccct tctgcagtgc atgaatgtag
1021ctcttcgacc aggccatgac ttgcttctgg cagtcaaaca gcaacagcag cgattcagtg
1081atttgcgaga gctttttgcc cggagactgg ccagtcacct caacaatgtt tttgttcaac
1141agggtcatga tcagagttcg actcttgccc aacactctgt tgaactgact ttacccaatc
1201atcatccatt tcatagagat ttgctccgat atgccaagct gatggagtgg ctaaagagta
1261cagattatgg aaaatatgaa ggactaacaa agaattacat ggattattta tcccgactat
1321atgaaagaga aatcaaagat ttctttgaag ttgcaaagat caagatgact ggcacaacta
1381aagaaagcaa gaagtttgct acactgcctc gaaaagaaag tgctgtcaaa caggaaacag
1441agagtcttca tggaagttcg gggaaattaa ctggatctac ttctagtcta aataagctca
1501gtgttcagag ttcagggaat cgcagatctc agtcatcttc cctgttggat atgggaaaca
1561tgtctgcctc tgatctcgat gttgctgaca ggaccaaatt tgataagatc tttgaacagg
1621tactaagtga actggagccc ctatgtctgg cagaacagga cttcataagt aaatttttca
1681aactacagca acatcaaagt atgcctggaa ctatggctga agcagaggac ctggatggag
1741gaacattatc acggcaacat aattgtggca caccactgcc tgtttcatct gagaaagata
1801tgatccgcca aatgatgatt aaaatatttc gctgcattga gccagagctg aacaacctaa
1861ttgcattagg agacaaaatt gatagcttta actctcttta tatgttagtc aaaatgagtc
1921atcatgtgtg gactgcacaa aatgtggacc ctgcttcttt cctaagtact acattgggaa
1981atgttttggt gactgtcaaa aggaactttg acaaatgcat tagtaaccaa ataaggcaaa
2041tggaagaagt aaagatctca aaaaagagta aagttggaat tcttccattt gttgctgaat
2101ttgaagaatt tgctggactt gcagaatcaa tcttcaaaaa tgctgagcgt cgtggagacc
2161tggataaagc atacaccaaa cttatcagag gagtatttgt taatgtggag aaagtagcaa
2221atgaaagcca gaagaccccc agggatgtgg ttatgatgga aaactttcac catatttttg
2281caactctttc tcgattgaaa atctcatgtc tagaagcaga aaaaaaagaa gccaaacaaa
2341aatacacaga tcaccttcag tcttatgtca tttactcttt aggacaacct cttGAAAAAC
2401TAAATCATTT CTTTGAAGGT GTTGAAGCTC GCGTGGCACA GGGCATAAGG GAGGAGGAAG
2461TAAGTTACCA ACTTGCATTT AACAAACAAG AACTTCGTAA AGTCATTAAG GAGTACCCTG
2521GAAAGGAAGT AAAAAAAGGT CTAGATAACC TCTACAAGAA AGTTGATAAA CATTTATGTG
2581AAGAAGAGAA CTTACTTCAG GTGGTGTGGC ACTCCATGCA AGATGAATTT ATACGCCAGT
2641ATAAGCACTT TGAAGGTTTG ATAGCTCGCT GTTATCCTGG ATCTGGTGTT ACAATGGAAT
2701TCACTATTCA GGACATTCTG GATTATTGTT CCAGCATTGC ACAGTCCCAC TTGCCAACTT
2761TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
2821CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
2881CTTGTGGAAA GGACGAGATA CTTTAGACAA AGATTTATGT ACGCGTTAAG TCgacaatca
2941acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)