Construct: ORF TRCN0000477387

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011807.1_s317c1
Derived from:
ccsbBroadEn_09929
DNA Barcode:
CTGTGATATGGCTGCATGTCTCTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SEC14L3 (266629)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477387
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 266629 SEC14L3 SEC14 like lipid binding 3 NM_174975.4 99.6% 99.2% (many diffs)
2 human 266629 SEC14L3 SEC14 like lipid binding 3 XM_011530128.2 90.3% 86% (many diffs)
3 human 266629 SEC14L3 SEC14 like lipid binding 3 NM_001257379.1 85% 84.7% (many diffs)
4 human 266629 SEC14L3 SEC14 like lipid binding 3 NM_001257382.1 85% 84.7% (many diffs)
5 human 266629 SEC14L3 SEC14 like lipid binding 3 NM_001257378.1 80.3% 80% (many diffs)
6 mouse 380683 Sec14l3 SEC14-like lipid binding 3 NM_001029937.2 90.4% 96.5% (many diffs)
7 mouse 380683 Sec14l3 SEC14-like lipid binding 3 XM_011243730.2 78.2% 83.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1269
ORF length:
1200
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gagcggccga gttggagacc tgagccccaa acaggcagag accctggcca
121agttccgaga aaacgtccag gatgtgcttc ctgccctgcc caaccctgat gattatttcc
181ttctacgctg gctccgagct cggaattttg acttgcagaa gtcggagact ttgctccgca
241agtacatgga gttccggaag accatggata ttgaccatat ccttgattgg cagcccccag
301aggtgatcca gaagtacatg cctgggggcc tgtgtggcta tgaccgtgat ggctgccccg
361tgtggtatga catcactggg ccacttgatc ccaaggggtt gctcttctca gtcaccaagc
421aggacctgct caagaccaag atgagggact gtgagcgcat cctgcatgag tgtgacctgc
481agacagagag gctagggaag aagattgaga ccatcgtgat gatatttgac tgtgagggcc
541tgggactgaa acacttctgg aaacctctgg tagaagtgta ccaggagttc tttggcctcc
601ttgaagagaa ttacccagag accctgaagt tcatgctcat cgtgaaagct accaaactgt
661tccctgtggg ctacaacctc atgaagccat tcctgagtga ggacactcgc aggaaaatta
721ttgtgttggg aaataactgg aaggaaggtt tgctgaaact catcagtcct gaggaactgc
781ctgcccagtt tgggggcacc ctgactgacc cagatgggaa ccccaaatgt ttaaccaaga
841ttaactatgg cggggagatc cCCAAGTCCA TGTACGTGCG GGACCAGGTG AAGACTCAGT
901ACGAGCACTC GGTGCAGATC AACCGCGGCT CATCACACCA AGTGGAATAC GAGATCCTAT
961TTCCAGGCTG CGTTCTCAGG TGGCAGTTCT CATCTGACGG TGCGGACATC GGCTTCGGAG
1021TTTTCCTGAA GACCAAGATG GGGGAGCGAC AGCGGGCAGG GGAGATGACA GAGGTTCTAC
1081CCAGCCAGCG CTATAACGCC CACATGGTGC CCGAGGATGG GAACCTCACC TGCTCAGAGG
1141CCGGCGTCTA TGTCCTACGC TTCGACAACA CCTATAGCTT TGTCCACGCC AAGAAGGTCA
1201GCTTCACAGT GGAGGTCCTG CTCCCTGACG AGGGCATGCA GAAATATGAT AAGGAGCTCA
1261CCCCTGTCTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
1321CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
1381TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACTGTGA TATGGCTGCA TGTCTCTCAC
1441GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)