Construct: ORF TRCN0000477393
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009497.1_s317c1
- Derived from:
- ccsbBroadEn_03246
- DNA Barcode:
- ACTATTTACATGGATTCATACAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAB9B (51209)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477393
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51209 | RAB9B | RAB9B, member RAS oncogene ... | NM_016370.4 | 100% | 100% | |
| 2 | mouse | 319642 | Rab9b | RAB9B, member RAS oncogene ... | NM_176971.2 | 92% | 98% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 672
- ORF length:
- 603
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagtgggaaa tccctgctct taaaggtcat tctcttgggt gatggtggag 121 ttgggaaaag ttcgcttatg aaccgttacg taaccaacaa atttgactcc caggcttttc 181 acaccatagg ggtagagttc ttaaatcgag atctggaggt agatggacgc tttgtaaccc 241 tccagatctg ggacactgca gggcaggaac gtttcaagag ccttaggaca cccttctaca 301 ggggagcaga ctgctgccTC TTGACCTTCA GCGTGGATGA TCGGCAGAGC TTCGAGAATC 361 TTGGTAACTG GCAGAAAGAA TTTATTTACT ATGCGGATGT GAAGGACCCT GAGCATTTCC 421 CCTTTGTAGT TCTGGGTAAC AAGGTAGACA AAGAGGATAG GCAAGTGACT ACTGAGGAGG 481 CACAAACCTG GTGCATGGAG AATGGGGATT ACCCTTATTT AGAAACTAGT GCCAAAGATG 541 ATACTAATGT GACAGTGGCC TTTGAAGAAG CTGTCAGGCA GGTGCTGGCT GTAGAGGAAC 601 AGCTGGAGCA TTGCATGTTG GGTCACACCA TTGACTTGAA CAGTGGCTCC AAAGCAGGGT 661 CTTCGTGCTG TTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 721 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 781 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAACT ATTTACATGG ATTCATACAG 841 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t