Construct: ORF TRCN0000477413
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013073.1_s317c1
- Derived from:
- ccsbBroadEn_02201
- DNA Barcode:
- GTCAACCAAAAGGACTTGCGATCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CLOCK (9575)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477413
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9575 | CLOCK | clock circadian regulator | NM_001267843.1 | 99.9% | 100% | 1764T>C |
| 2 | human | 9575 | CLOCK | clock circadian regulator | NM_004898.4 | 99.9% | 100% | 1764T>C |
| 3 | human | 9575 | CLOCK | clock circadian regulator | XM_005265787.2 | 99.9% | 100% | 1764T>C |
| 4 | human | 9575 | CLOCK | clock circadian regulator | XM_011534409.2 | 99.9% | 100% | 1764T>C |
| 5 | human | 9575 | CLOCK | clock circadian regulator | XM_011534410.2 | 99.9% | 100% | 1764T>C |
| 6 | human | 9575 | CLOCK | clock circadian regulator | XM_011534411.2 | 99.9% | 100% | 1764T>C |
| 7 | human | 9575 | CLOCK | clock circadian regulator | XM_017008854.1 | 99.9% | 100% | 1764T>C |
| 8 | human | 9575 | CLOCK | clock circadian regulator | XM_024454284.1 | 99.9% | 100% | 1764T>C |
| 9 | human | 9575 | CLOCK | clock circadian regulator | XM_017008855.1 | 92.9% | 93% | 0_1ins177;1587T>C |
| 10 | mouse | 12753 | Clock | circadian locomotor output ... | NM_001305222.1 | 89.2% | 94.6% | (many diffs) |
| 11 | mouse | 12753 | Clock | circadian locomotor output ... | XM_017320649.1 | 89.2% | 94.6% | (many diffs) |
| 12 | mouse | 12753 | Clock | circadian locomotor output ... | NM_001289826.1 | 89.1% | 94.5% | (many diffs) |
| 13 | mouse | 12753 | Clock | circadian locomotor output ... | NM_007715.6 | 89.1% | 94.5% | (many diffs) |
| 14 | mouse | 12753 | Clock | circadian locomotor output ... | XM_017320648.1 | 89.1% | 94.5% | (many diffs) |
| 15 | mouse | 12753 | Clock | circadian locomotor output ... | XM_017320651.1 | 85.9% | 91.5% | (many diffs) |
| 16 | mouse | 12753 | Clock | circadian locomotor output ... | XM_017320650.1 | 85.8% | 91.3% | (many diffs) |
| 17 | mouse | 12753 | Clock | circadian locomotor output ... | XM_011249402.1 | 75.5% | 80.2% | (many diffs) |
| 18 | mouse | 12753 | Clock | circadian locomotor output ... | XR_001784602.1 | 22.9% | (many diffs) | |
| 19 | mouse | 12753 | Clock | circadian locomotor output ... | XR_001784601.1 | 22.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2607
- ORF length:
- 2538
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gttgtttacc gtaagctgta gtaaaatgag ctcgattgtt gacagagatg 121 acagtagtat ttttgatggg ttggtggaag aagatgacaa ggacaaagcg aaaagagtat 181 ctagaaacaa atctgaaaag aaacgtagag atcaatttaa tgttctcatt aaagaactgg 241 gatccatgct tcctggtaat gctagaaaga tggacaaatc tactgttctg cagaaaagca 301 ttgatttttt acgaaaacat aaagaaatca ctgcacagtc agatgctagt gaaattcgac 361 aggactggaa acctacattc cttagtaatg aagagtttac acaattaatg ttagaggctc 421 ttgatggttt ttttttagca atcatgacag atggaagcat aatatatgtg tctgagagtg 481 taacttcatt acttgaacat ttaccatctg atcttgtgga tcaaagtata tttaatttta 541 tcccagaagg ggaacattca gaggtttata aaatactctc tactcatctg ctggaaagtg 601 attcattaac cccagaatat ttaaaatcaa aaaatcagtt agaattctgt tgtcacatgc 661 tgcgaggaac aatagaccca aaggagccat ctacctatga atatgtaaaa tttataggaa 721 atttcaaatc tttaaacagt gtatcctctt cagcacacaa tggttttgaa ggaactatac 781 aacgcacaca taggccatct tatgaagata gagtttgttt tgtagctact gtcaggttag 841 ctacacctca gttcatcaag gaaatgtgca ctgttgaaga acccaatgaa gagtttacat 901 ctagacatag tttagaatgg aagtttctgt ttctagatca cagggcacca cccataatag 961 ggtatttgcc atttgaagtt ctgggaacat caggctatga ttactatcat gtggatgacc 1021 tagaaaattt ggcaaaatgt catgagcact taatgcaata tgggaaaggc aaatcatgtt 1081 attataggtt cctgactaag gggcaacagt ggatttggct tcagactcat tattatatca 1141 cttaccatca gtggaattca aggccagagt ttattgtttg tactcacact gtagtaagtt 1201 atgcagaagt tagggctgaa agacgacgag aacttggcat tgaagagtct cttcctgaga 1261 cagctgctga caaaagccaa gattctgggt cagataatcg tataaacaca gtcagtctca 1321 aggaagcatt ggaaaggttt gatcacagcc caaccccttc tgcctcttct cggagttcaa 1381 gaaaatcatc tcacacggcc gtctcagacc cttcctcaac accaaccaag atcccgacgg 1441 atacgagcac tccacccagg cagcatttac cagctcatga gaagatggtg caaagaaggt 1501 catcatttag tagtcagtcc ataaattccc agtctgttgg ttcatcatta acacagccag 1561 tgatgtctca agctacaaat ttaccaattc cacaaggcat gtcccagttt cagttttcag 1621 ctcaattagg agccatgcaa catctgaaag accaattgga acaacggaca cgcatgatag 1681 aagcaaatat tcatcggcaa caagaagaac taagaaaaat tcaagaacaa cttcagatgg 1741 tccatggtca ggggctgcag atgtttttgc aacaatcaaa tcctgggttg aattttggtt 1801 ccgttcaact ttcttctgga aattcatcta acatccagca acttgcacct ataaatatgc 1861 aaggccaagt tgttcctact aaccagattc aaagtggaat gaatactgga cacattggca 1921 caactcagca catgatacaa caacagactt tacagagtac atcaactcag agtcaacaaa 1981 atgtactgag tgggcacagt cagcaaacat ctctacccag tcagacacag agcactctta 2041 cagccccact gtataacact atggtgattt ctcagcctgc agccggaagc atggTCCAGA 2101 TTCCATCTAG TATGCCACAA AACAGCACCC AGAGTGCTGC AGTAACTACA TTCACTCAGG 2161 ACAGGCAGAT AAGATTTTCT CAAGGTCAAC AACTTGTGAC CAAATTAGTG ACTGCTCCTG 2221 TAGCTTGTGG GGCAGTCATG GTACCTAGTA CTATGCTTAT GGGCCAGGTG GTGACTGCAT 2281 ATCCTACTTT TGCTACACAA CAGCAACAGT CACAGACATT GTCAGTAACG CAGCAGCAGC 2341 AGCAGCAGAG CTCCCAGGAG CAGCAGCTCA CTTCAGTTCA GCAACCATCT CAGGCTCAGC 2401 TGACCCAGCC ACCGCAACAA TTTTTACAGA CTTCTAGGTT GCTCCATGGG AATCCCTCAA 2461 CTCAACTCAT TCTCTCTGCT GCATTTCCTC TACAACAGAG CACCTTCCCT CAGTCACATC 2521 ACCAGCAACA TCAGTCTCAG CAACAGCAGC AACTCAGCCG GCACAGGACT GACAGCTTGC 2581 CCGACCCTTC CAAGGTTCAA CCACAGTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 2641 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2701 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGTCAACCA 2761 AAAGGACTTG CGATCTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2821 aagatt