Construct: ORF TRCN0000477414
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016718.1_s317c1
- Derived from:
- ccsbBroadEn_07392
- DNA Barcode:
- GCTGTCCCCGGGACTGGGAACTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TGM5 (9333)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477414
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9333 | TGM5 | transglutaminase 5 | NM_201631.4 | 99.9% | 99.8% | 326C>A;951T>C |
| 2 | human | 9333 | TGM5 | transglutaminase 5 | NM_004245.4 | 88.5% | 88.6% | 189_190ins246;705T>C |
| 3 | human | 9333 | TGM5 | transglutaminase 5 | XM_011522230.2 | 52.3% | 52.3% | 0_1ins1029 |
| 4 | human | 9333 | TGM5 | transglutaminase 5 | XM_017022729.1 | 52.3% | 52.3% | 0_1ins1029 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2229
- ORF length:
- 2160
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcccaaggg ctagaagtgg ccctcacaga cctccagagc tccagaaata 121 atgtgcggca ccacacggag gagatcactg tggaccacct gcttgttcgc cggggccagg 181 ccttcaacct caccctgtac ttcaggaacc ggagcttcca gccaggcctg gacaacatca 241 tcttcgtggt tgaaactgga ccgctgccag acctggcctt ggggactcgg gctgtgttca 301 gcctggcacg ccatcacagc cccagcccct ggattgcctg gctggagacc aatggggcca 361 cctccacaga ggtgagcttg tgcgctcctc ccaaggcggc cgtgggtcgg tacctcttga 421 aaatccacat cgactccttc caggggtctg tgacggccta ccagctaggg gagttcatcc 481 tgcttttcaa tccctggtgc ccagaggatg ctgtctactt ggacagtgaa ccccagaggc 541 aggagtatgt catgaatgat tatggcttca tctaccaagg cagcaagaac tggatccgcc 601 catgtccctg gaactatgga cagtttgaag acaaaatcat agacatctgc ctgaagctgc 661 tagacaagag cctgcacttc cagactgacc cagccacaga ctgtgctctg cggggaagcc 721 ccgtctacgt cagcagagtg gtgtgtgcca tgatcaacag caatgatgat aatggggtgc 781 tcaatggaaa ctggagtgag aattacacag acggcgccaa ccctgcggag tggacgggca 841 gcgtggccat cctgaagcag tggaacgcca caggctgcca gcccgtgcgc tacgggcaat 901 gctgggtctt tgctgccgtc atgtgcacag tgatgaggtg tctggggatc cctacccgtg 961 tgatcaccaa cttcgactct ggccacgata cagatggaaa cctgatcata gatgagtact 1021 atgacaacac aggcaggatt ttggggaata agaagaagga tactatctgg aacttccatg 1081 tctggaatga gtgctggatg gcccggaagg atctgccccc tgcatatgga ggctggcagg 1141 tgctggacgc cacacctcag gagatgagca acggcgtcta ctgctgtggc cctgcctctg 1201 tcagagccat caaagaagga gaagtggacc tgaactatga cacgcccttt gtgttttcga 1261 tggtgaatgc tgactgcatg tcctggctcg tccagggagg gaaggagcag aagcttcacc 1321 aggacacgag ttctgttggc aattttatca gcacaaagag catccagagt gacgagcggg 1381 atgacatcac agagaactac aagtatgaag aaggatccct ccaggagagg caggtgtttc 1441 tgaaggctct gcagaagctg aaggctagaa gcttccatgg ctcccaaaga ggagcagagt 1501 tgcaaccttc caggcccaca tcactgagcc aggacagccc tcggagcctg catacacctt 1561 cccttcgacc cagtgatgtg gtgcaagtct ccctgaaatt caagctgctc gacccgccca 1621 acatgggcca ggatatatgc tttgtcctgc tggccctcaa catgtcctcc cagttcaagg 1681 acctcaaagt gaacctgagt gcccagtctc tgctgcacga tggcagcccc ctgtccccat 1741 tctggcagga cacagcgttc atcacactct ctcctaaaga agcaaagacc tacccctgca 1801 aaaTCTCCTA TTCCCAGTAC AGCCAGTACC TGTCAACAGA CAAGCTGATC CGCATCAGTG 1861 CCCTGGGTGA AGAGAAAAGC AGTCCTGAGA AAATCCTGGT GAACAAGATC ATCACCTTAT 1921 CTTATCCAAG CATCACGATT AATGTTCTAG GAGCAGCCGT TGTGAACCAG CCACTCTCCA 1981 TACAGGTGAT ATTTTCAAAC CCCCTCTCGG AGCAGGTTGA GGACTGTGTG CTGACTGTGG 2041 AAGGAAGTGG CCTCTTCAAG AAACAGCAGA AAGTCTTCCT TGGAGTCCTC AAACCCCAAC 2101 ACCAAGCAAG CATCATTCTG GAGACCGTCC CCTTCAAGAG TGGACAAAGG CAGATCCAAG 2161 CTAATATGAG AAGCAACAAG TTTAAGGACA TTAAGGGTTA CAGGAATGTT TATGTAGACT 2221 TTGCATTATT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 2281 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 2341 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGCTGTC CCCGGGACTG GGAACTTTAC 2401 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt