Construct: ORF TRCN0000477428
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003110.1_s317c1
- Derived from:
- ccsbBroadEn_00840
- DNA Barcode:
- GTTTCAAAAAATCTAATCGCTTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IGFBP7 (3490)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477428
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3490 | IGFBP7 | insulin like growth factor ... | NM_001553.3 | 100% | 100% | |
| 2 | human | 3490 | IGFBP7 | insulin like growth factor ... | NM_001253835.1 | 98.1% | 98.2% | 832_835delACACinsG;837_838insGGTGCCGAGCTA |
| 3 | mouse | 29817 | Igfbp7 | insulin-like growth factor ... | NM_008048.3 | 90% | 90.8% | (many diffs) |
| 4 | mouse | 29817 | Igfbp7 | insulin-like growth factor ... | NM_001159518.1 | 81.2% | 81.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 912
- ORF length:
- 846
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gcggccgtcg ctgcgcgccc tgctcctcgg cgccgctggg ctgctgctcc 121 tgctcctgcc cctctcctct tcctcctctt cggacacctg cggcccctgc gagccggcct 181 cctgcccgcc cctgcccccg ctgggctgcc tgctgggcga gacccgcgac gcgtgcggct 241 gctgccctat gtgcgcccgc ggcgagggcg agccgtgcgg gggtggcggc gccggcaggg 301 ggtactgcgc gccgggcatg gagtgcgtga agagccgcaa gaggcggaag ggtaaagccg 361 gggcagcagc cggcggtccg ggtgtaagcg gcgtgtgcgt gtgcaagagc cgctacccgg 421 tgtgcggcag cgacggcacc acctacccga gcggctgcca gctgcgcgcc gccagccaga 481 gggccgagag ccgcggggag aaggccatca cccaggtcag caagggcacc tgcgagcaag 541 gtccttccat agtgacgccc cccaaggaca tctggaatgt cactggtgcC CAGGTGTACT 601 TGAGCTGTGA GGTCATCGGA ATCCCGACAC CTGTCCTCAT CTGGAACAAG GTAAAAAGGG 661 GTCACTATGG AGTTCAAAGG ACAGAACTCC TGCCTGGTGA CCGGGACAAC CTGGCCATTC 721 AGACCCGGGG TGGCCCAGAA AAGCATGAAG TAACTGGCTG GGTGCTGGTA TCTCCTCTAA 781 GTAAGGAAGA TGCTGGAGAA TATGAGTGCC ATGCATCCAA TTCCCAAGGA CAGGCTTCAG 841 CATCAGCAAA AATTACAGTG GTTGATGCCT TACATGAAAT ACCAGTGAAA AAAGGTGAAG 901 GTGCCGAGCT ATACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 961 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1021 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGTT TCAAAAAATC TAATCGCTTC 1081 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t