Construct: ORF TRCN0000477446
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001928.1_s317c1
- Derived from:
- ccsbBroadEn_11405
- DNA Barcode:
- TGTCGGTGAGTGCAAGTAGTTATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UBE3C (9690)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477446
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9690 | UBE3C | ubiquitin protein ligase E3C | NM_014671.3 | 59.1% | 58.8% | (many diffs) |
| 2 | human | 9690 | UBE3C | ubiquitin protein ligase E3C | XM_017012818.1 | 57.2% | 56.8% | (many diffs) |
| 3 | human | 9690 | UBE3C | ubiquitin protein ligase E3C | XM_005249564.4 | 56.8% | 56.5% | (many diffs) |
| 4 | human | 9690 | UBE3C | ubiquitin protein ligase E3C | XR_927552.3 | 38% | (many diffs) | |
| 5 | mouse | 100763 | Ube3c | ubiquitin protein ligase E3C | NM_133907.3 | 51.4% | 53.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2013
- ORF length:
- 1947
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt cagcttcgaa ggcgacttca agacgcggcc caaggtgtcc cttggcggcg 121 cgagcaggaa ggaggaaaag gcttctcttt tacatcgtac tcaggaagaa agaagaaaga 181 gagaggaaga aaggcgaagg ttgaaaaatg caataattat ccagtcattt attcgaggct 241 atagagacag aaaacagcaa tattccatcc aaagaagtgc atttgatcgc tgtgctacct 301 tgtcacagtc cgggggcgct tttcccattg ctaatggccc caaccttacc cttttggtaa 361 ggcagcttct gtttttttac aaacaaaatg aagactcaaa acgtttgata tggctgtatc 421 agaacttaat taaacacagc tctctgtttg tcaagcagtt ggatggatct gagagactta 481 catgcttatt tcagataaaa agattgatga gcctctgttg caggttgctg caaaactgta 541 atgatgacag tttgaatgtt gcacttccaa tgagaatgct tgaagtattt tcgtctgaga 601 atacttactt gcctgtttta caagatgcta gctatgtggt gtcagtgatt gaacaaattt 661 tgcactacat gattcacaat gggtattata ggtctctata tttgttgatt aacagcaagc 721 ttccatcaag tattgaatat tctgatttat ctcgagttcc tatagcaaaa attttgctag 781 agaatgttct aaaaccattg cactttactt acaactcctg tccggaaggt gcgaggcaac 841 aagtttttac agccttcaca gaggagtttc tggcagcacc ttttacagat cagatttttc 901 atttcatcat tccggcgctt gcagatgcgc agaccgtttt cccttacgag ccctttctga 961 atgcactgtt gttaatagag agtagatgtt caagaaagag tggtggagca ccctggcttt 1021 tctatttcgt tttaactgtt ggcgaaaatt atttgggggc cctctctgag gaagggctgc 1081 tggtgtattt gcgggtgctg cagaccttcc tctctcagtt accagtctct cctgccagcg 1141 cgagctgtca cgactcagcc agtgactctg aggaggagag tgaagaagcc gacaagccct 1201 caagcccgga ggatggcaga ctgtcagtat catacataac agaggaatgc ctgaagaagc 1261 tggacacaaa gcagcagacc aacaccctgc tcaacctggt gtggagggac tctgcgagcg 1321 aggaggtctt caccaccatg gcctccgtct gccacacgct gatggtgcag caccgcatga 1381 tggtacccaa agtcaggctt ctctacagtt tagcctttaa tgccaggttt ctgagacatc 1441 tttggtttct aatatcttcc atgtcaacac ggatgatcac agggtctatg gtaccgttgc 1501 ttcaggtgat atccaggggt tctcctatgt cttttgaaga ttctagtcga atcatcccac 1561 tcttttacct ttttagctCC TTGTTTAGTC ATTCACTAAT TTCCATACAT GATAACGAAT 1621 TCTTCGGTGA TCCCATAGAA GTTGTAGGTC AAAGACAATC ATCAATGATG CCTTTTACTT 1681 TAGAAGAGCT GATAATGTTG TCTCGATGCC TTCGAGATGC ATGCCTGGGG ATCATCAAGT 1741 TGGCTTATCC AGAAACCAAG CCAGAAGTTC GAGAAGAATA TATTACAGCA TTTCAGAGTA 1801 TTGGAGTTAC TACTAGCTCT GAAATGCAAC AATGCATACA GATGGAACAG AAAAGATGGA 1861 TTCAGTTATT TAAGGTTATC ACCAATCTAG TGAAAATGTT GAAGTCCAGA GACACGAGGA 1921 GAAATTTTTG TCCTCCAAAC CACTGGCTGT CAGAACAAGA AGATATTAAA GCAGATAAGG 1981 TGTTATTGAA AGATCTTTTT AATATTTATC ATTACCCAAC TTTCTTGTAC AAAGTGGTTG 2041 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2101 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATG 2161 TCGGTGAGTG CAAGTAGTTA TAACGCGTTA AGTCgacaat caacctctgg attacaaaat 2221 ttgtgaaaga tt