Construct: ORF TRCN0000477477
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000643.1_s317c1
- Derived from:
- ccsbBroadEn_07864
- DNA Barcode:
- TCTCTCGCAAATTAAACTGCAGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WSCD1 (23302)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477477
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23302 | WSCD1 | WSC domain containing 1 | NM_015253.1 | 99.8% | 99.6% | 634C>T;1095A>G;1715A>C |
| 2 | human | 23302 | WSCD1 | WSC domain containing 1 | XM_005256572.2 | 99.8% | 99.6% | 634C>T;1095A>G;1715A>C |
| 3 | human | 23302 | WSCD1 | WSC domain containing 1 | XM_005256573.1 | 99.8% | 99.6% | 634C>T;1095A>G;1715A>C |
| 4 | human | 23302 | WSCD1 | WSC domain containing 1 | XM_024450682.1 | 99.8% | 99.6% | 634C>T;1095A>G;1715A>C |
| 5 | human | 23302 | WSCD1 | WSC domain containing 1 | XM_011523777.2 | 53.1% | 50.6% | (many diffs) |
| 6 | mouse | 216881 | Wscd1 | WSC domain containing 1 | NM_177618.4 | 81.7% | 85.2% | (many diffs) |
| 7 | mouse | 216881 | Wscd1 | WSC domain containing 1 | XM_006532928.3 | 81.7% | 85.2% | (many diffs) |
| 8 | mouse | 216881 | Wscd1 | WSC domain containing 1 | XM_006532929.3 | 81.7% | 85.2% | (many diffs) |
| 9 | mouse | 216881 | Wscd1 | WSC domain containing 1 | XM_006532930.3 | 81.7% | 85.2% | (many diffs) |
| 10 | mouse | 216881 | Wscd1 | WSC domain containing 1 | XM_006532931.3 | 81.7% | 85.2% | (many diffs) |
| 11 | mouse | 216881 | Wscd1 | WSC domain containing 1 | XM_006532932.3 | 49.7% | 52.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1791
- ORF length:
- 1725
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc caaacctttc ttccgactcc agaagtttct ccgccgaaca cagttcctgc 121 tgttcttcct cacggctgcc tacctgatga ccggcagcct gctgctgctg cagcgggtcc 181 gcgtggctct cccacagggc ccccgggcac ccggccccct gcagaccttg ccagtggccg 241 ccgtggcgct gggcgtgggc ttgctggaca gcagagccct gcacgaccct cgagtcagcc 301 cagagctgct gctgggtgtg gacatgctgc agagccccct gacccggccc cggcccggcc 361 cccgctggct ccggagccgc aactcggagc tgcgtcagtt gcgtcgccgc tggttccacc 421 acttcatgag tgactcccag ggaccgcccg ccctgggccc cgaggctgcc aggcccgcca 481 tccacagccg aggcacctac attggatgct tcagtgacga tggccacgag aggactctga 541 aaggagctgt gttttatgac ttgagaaaga tgactgtctc ccactgccag gatgcgtgtg 601 ctgagcggtc ctatgtctac gccggcttgg aggccggggc ggagtgttac tgcgggaacc 661 ggctgccagc ggtgagcgtg gggctggaag agtgtaacta tgagtgcaaa ggcgagaagg 721 gctctgtgtg cggggctgtg gaccggctct ccgtgtaccg tgtggacgag ctgcagccgg 781 gctccaggaa gcggcggacc gccacctacc gcggatgctt ccgactgcca gagaacatca 841 cacatgcctt ccccagctcc ctgatacagg ccaatgtgac cgtggggact tgctcgggct 901 tttgttccca gaaagagttc cccttggcca ttctcagggg ctgggaatgc tactgtgctt 961 accctacccc ccggttcaac ctgcgggatg ccatggacag ctcagtatgt ggccaggacc 1021 ctgaggcaca gaggctggca gaatactgtg aggtctacca gacacctgtg caagacactc 1081 gttgtacaga caggaggttc ctgcctaaca aatccaaagt gtttgtggct ttgtcaagct 1141 tcccaggagc cgggaacacg tgggcacggc acctcattga gcatgccact ggcttctata 1201 cagggagcta ctactttgat ggaaccctct acaacaaagg gttcaagggc gaaaaggacc 1261 actggcggag ccgacgcacc atctgtgtca aaacccacga gagtggcagg agggagattg 1321 agatgtttga ttcagccatc ctgctaatcc ggaacccata caggtccctg gtggcagaat 1381 tcaacagaaa atgtgccggg cacctGGGAT ATGCAGCTGA CCGCAACTGG AAGAGCAAAG 1441 AGTGGCCGGA CTTTGTCAAC AGCTACGCCT CGTGGTGGTC CTCGCACGTC CTGGACTGGC 1501 TCAAGTACGG GAAGCGGCTG CTGGTGGTGC ACTACGAGGA GCTGCGGCGC AGCCTGGTGC 1561 CCACGTTACG GGAGATGGTG GCCTTCCTCA ACGTGTCTGT GAGCGAGGAG CGGCTGCTCT 1621 GCGTGGAGAA CAACAAGGAG GGCAGCTTCC GGCGGCGCGG CCGGCGCTCC CACGACCCTG 1681 AGCCCTTCAC CCCGGAGATG AAAGACTTGA TCAATGGCTA CATCCGGACG GTGGACCAAG 1741 CCCTGCGTGA CCACAACTGG ACGGGGCTGC CCAGGGAGTC TGTGCCCAGA TGCCCAACTT 1801 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1861 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1921 CTTGTGGAAA GGACGATCTC TCGCAAATTA AACTGCAGCC ACGCGTTAAG TCgacaatca 1981 acctctggat tacaaaattt gtgaaagatt