Construct: ORF TRCN0000477477

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000643.1_s317c1
Derived from:
ccsbBroadEn_07864
DNA Barcode:
TCTCTCGCAAATTAAACTGCAGCC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
WSCD1 (23302)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477477
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23302 WSCD1 WSC domain containing 1 NM_015253.1 99.8% 99.6% 634C>T;1095A>G;1715A>C
2 human 23302 WSCD1 WSC domain containing 1 XM_005256572.2 99.8% 99.6% 634C>T;1095A>G;1715A>C
3 human 23302 WSCD1 WSC domain containing 1 XM_005256573.1 99.8% 99.6% 634C>T;1095A>G;1715A>C
4 human 23302 WSCD1 WSC domain containing 1 XM_024450682.1 99.8% 99.6% 634C>T;1095A>G;1715A>C
5 human 23302 WSCD1 WSC domain containing 1 XM_011523777.2 53.1% 50.6% (many diffs)
6 mouse 216881 Wscd1 WSC domain containing 1 NM_177618.4 81.7% 85.2% (many diffs)
7 mouse 216881 Wscd1 WSC domain containing 1 XM_006532928.3 81.7% 85.2% (many diffs)
8 mouse 216881 Wscd1 WSC domain containing 1 XM_006532929.3 81.7% 85.2% (many diffs)
9 mouse 216881 Wscd1 WSC domain containing 1 XM_006532930.3 81.7% 85.2% (many diffs)
10 mouse 216881 Wscd1 WSC domain containing 1 XM_006532931.3 81.7% 85.2% (many diffs)
11 mouse 216881 Wscd1 WSC domain containing 1 XM_006532932.3 49.7% 52.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1791
ORF length:
1725
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc caaacctttc ttccgactcc agaagtttct ccgccgaaca cagttcctgc
121tgttcttcct cacggctgcc tacctgatga ccggcagcct gctgctgctg cagcgggtcc
181gcgtggctct cccacagggc ccccgggcac ccggccccct gcagaccttg ccagtggccg
241ccgtggcgct gggcgtgggc ttgctggaca gcagagccct gcacgaccct cgagtcagcc
301cagagctgct gctgggtgtg gacatgctgc agagccccct gacccggccc cggcccggcc
361cccgctggct ccggagccgc aactcggagc tgcgtcagtt gcgtcgccgc tggttccacc
421acttcatgag tgactcccag ggaccgcccg ccctgggccc cgaggctgcc aggcccgcca
481tccacagccg aggcacctac attggatgct tcagtgacga tggccacgag aggactctga
541aaggagctgt gttttatgac ttgagaaaga tgactgtctc ccactgccag gatgcgtgtg
601ctgagcggtc ctatgtctac gccggcttgg aggccggggc ggagtgttac tgcgggaacc
661ggctgccagc ggtgagcgtg gggctggaag agtgtaacta tgagtgcaaa ggcgagaagg
721gctctgtgtg cggggctgtg gaccggctct ccgtgtaccg tgtggacgag ctgcagccgg
781gctccaggaa gcggcggacc gccacctacc gcggatgctt ccgactgcca gagaacatca
841cacatgcctt ccccagctcc ctgatacagg ccaatgtgac cgtggggact tgctcgggct
901tttgttccca gaaagagttc cccttggcca ttctcagggg ctgggaatgc tactgtgctt
961accctacccc ccggttcaac ctgcgggatg ccatggacag ctcagtatgt ggccaggacc
1021ctgaggcaca gaggctggca gaatactgtg aggtctacca gacacctgtg caagacactc
1081gttgtacaga caggaggttc ctgcctaaca aatccaaagt gtttgtggct ttgtcaagct
1141tcccaggagc cgggaacacg tgggcacggc acctcattga gcatgccact ggcttctata
1201cagggagcta ctactttgat ggaaccctct acaacaaagg gttcaagggc gaaaaggacc
1261actggcggag ccgacgcacc atctgtgtca aaacccacga gagtggcagg agggagattg
1321agatgtttga ttcagccatc ctgctaatcc ggaacccata caggtccctg gtggcagaat
1381tcaacagaaa atgtgccggg cacctGGGAT ATGCAGCTGA CCGCAACTGG AAGAGCAAAG
1441AGTGGCCGGA CTTTGTCAAC AGCTACGCCT CGTGGTGGTC CTCGCACGTC CTGGACTGGC
1501TCAAGTACGG GAAGCGGCTG CTGGTGGTGC ACTACGAGGA GCTGCGGCGC AGCCTGGTGC
1561CCACGTTACG GGAGATGGTG GCCTTCCTCA ACGTGTCTGT GAGCGAGGAG CGGCTGCTCT
1621GCGTGGAGAA CAACAAGGAG GGCAGCTTCC GGCGGCGCGG CCGGCGCTCC CACGACCCTG
1681AGCCCTTCAC CCCGGAGATG AAAGACTTGA TCAATGGCTA CATCCGGACG GTGGACCAAG
1741CCCTGCGTGA CCACAACTGG ACGGGGCTGC CCAGGGAGTC TGTGCCCAGA TGCCCAACTT
1801TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
1861CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
1921CTTGTGGAAA GGACGATCTC TCGCAAATTA AACTGCAGCC ACGCGTTAAG TCgacaatca
1981acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)