Construct: ORF TRCN0000477478
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014928.1_s317c1
- Derived from:
- ccsbBroadEn_02735
- DNA Barcode:
- AGACCTCGAGCGATCGCGGAACCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAF2 (23197)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477478
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23197 | FAF2 | Fas associated factor famil... | NM_014613.3 | 100% | 100% | |
| 2 | human | 23197 | FAF2 | Fas associated factor famil... | XM_011534475.3 | 89.8% | 89.8% | 131_132ins135 |
| 3 | mouse | 76577 | Faf2 | Fas associated factor famil... | NM_178397.3 | 90.4% | 97.7% | (many diffs) |
| 4 | mouse | 76577 | Faf2 | Fas associated factor famil... | XM_006517452.3 | 84.6% | 91.6% | (many diffs) |
| 5 | mouse | 76577 | Faf2 | Fas associated factor famil... | XM_011244582.2 | 84.6% | 91.6% | (many diffs) |
| 6 | mouse | 76577 | Faf2 | Fas associated factor famil... | XM_011244583.2 | 84.6% | 91.6% | (many diffs) |
| 7 | mouse | 76577 | Faf2 | Fas associated factor famil... | XM_017315634.1 | 47.7% | 53.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1404
- ORF length:
- 1335
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggcgcct gaggagcggg atctaaccca ggagcagaca gagaagctgc 121 tgcagtttca ggatctcact ggcatcgaat ctatggatca gtgtcgccat accttggaac 181 agcataactg gaacatagag gctgctgtac aggacagatt gaatgagcaa gagggcgtac 241 ctagtgtttt caacccacct ccatcacgac ccctgcaggt taatacagct gaccacagga 301 tctacagcta tgttgtctca agacctcaac caagggggct gcttggatgg ggttattact 361 tgataatgct tccattccgg tttacctatt acacgatact tgatatattt aggtttgctc 421 ttcgttttat acggcctgac cctcgcagcc gggtcactga ccccgttggg gacattgttt 481 catttatgca ctcttttgaa gagaaatatg ggagggcaca ccctgtcttc taccagggaa 541 cgtacagcca ggcacttaac gatgccaaaa gggagcttcg ctttcttttg gtttatcttc 601 atggagatga tcaccaggac tctgatgagt tttgtcgcaa cacactctgt gcacctgaag 661 ttatttcact aataaacact aggatgctct tctgggcatg ctctacaaac aaacctgagg 721 gatacagggt ctcacaggct ttacgagaga acacctatcc attcctggcc atgattatgc 781 tgaaggatcg aaggatgact gtggtgggac ggctagaagg cctcattcaa cctgatgacc 841 tcattaacca actgacattt atcatggatg ctaaccagac ttacctggtg tcagaacgcc 901 tagaaaggga agaaagaaac cagacccaag tgctgagaca acagcaggat gaggcctacc 961 tggcctctcT CAGAGCTGAC CAGGAGAAAG AAAGAAAGAA ACGGGAGGAG CGGGAGCGTA 1021 AGCGGCGGAA GGAGGAGGAG GTGCAACAGC AAAAGTTGGC AGAGGAGAGA CGGCGGCAGA 1081 ATTTACAGGA GGAAAAGGAA AGGAAGTTGG AATGCCTGCC CCCTGAACCT TCCCCTGATG 1141 ACCCTGAAAG TGTCAAGATC ATCTTCAAAT TACCTAATGA TTCTCGAGTA GAGAGACGAT 1201 TCCACTTTTC ACAGTCTCTA ACAGTAATCC ACGACTTCTT ATTCTCCTTG AAGGAAAGCC 1261 CAGAAAAGTT TCAGATTGAA GCCAATTTTC CCAGGCGAGT GCTGCCCTGC ATCCCTTCAG 1321 AGGAGTGGCC CAATCCCCCT ACGCTACAGG AGGCCGGACT CAGCCACACA GAAGTTCTTT 1381 TTGTTCAGGA CCTAACTGAC GAATTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1441 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1501 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA GACCTCGAGC 1561 GATCGCGGAA CCCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1621 att